BDP 576/589 (a BODIPY™ 576/589 analog) is a boron dipyrromethene dye with emission in the orange spectrum range. This dye belongs to the BDP family and has expressed hydrophobicity, high quantum yield, and high molar extinction coefficient. The pyrrole ring in the molecule increases the π-conjugation area and participates in the formation of fluorophore spectral characteristics (i. e. moves the absorption and emission spectra in the long-wave region). Our reactive dye derivatives contain C3-linker between the fluorophore molecule and functional group.
This fluorophore has a relatively long excited-state lifetime (about 5 nanoseconds) and marked sensitivity to changes in microenvironment polarity, so it can be used in experiments measuring fluorescence lifetime and in fluorescence anisotropy assays. Because of its large two-photon absorption cross-section, this dye can be also used in two-photon excitation microscopy.
BDP 576/589 has a Stokes shift of 13 nm and a narrow emission spectrum, which makes it an attractive fluorophore for various fluorescent microscopy types.