BDP 576/589 tetrazine

Cat. # Quantity Price Lead time
1G4E0 1 mg $110.00 in stock
2G4E0 5 mg $290.00 in stock
3G4E0 10 mg $450.00 in stock
4G4E0 25 mg $690.00 in stock
5G4E0 50 mg $1270.00 in stock
6G4E0 100 mg $1990.00 in stock

Because of its relatively long excited-state lifetime (about 5 nanoseconds), BDP 576/589 dye can be used in various methods based on measuring fluorescence lifetime. Similarly to other dyes of the BDP family, BDP 576/589 has strong hydrophobic properties and is suitable for labeling non-polar and lypophilic biomolecules and their subsequent visualization by fluorescent microscopy, including two-photon microscopy.

This reagent is a tetrazine derivative that can be conjugated with various strained dienophiles such as trans-cyclooctenes and cyclopropenes. This reaction (TCO ligation) is considered to be one of the best bioconjugation reactions because it is very fast and selective in physiological conditions and does not require additional catalyzers, so it is not toxic in vitro and in vivo.

BDP 576/589 absorbance and emission spectra

BDP 576/589 absorbance and emission spectra

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BDP 576/589 carboxylic acid

Derivative of BDP 576/589 fluorophore (BODIPY™ 576/589 analog) with non-activated free carboxylic acid group.
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BDP 581/591 tetrazine

BDP 581/591 is a borondipyrromethene dye with tetrazine group for the inverse electron demand Diels-Alder (IEDDA) ligation.

BDP 576/589 NHS ester

BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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General properties

Appearance: dark colored solid
Mass spec M+ increment: 501.16
Molecular weight: 529.17
Molecular formula: C26H23N8BF2O
Solubility: good in polar organic solvents
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 580
ε, L⋅mol−1⋅cm−1: 98000
Emission maximum, nm: 592
Fluorescence quantum yield: 0.13
CF260: 0.32
CF280: 0.35
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