BDP 576/589 tetrazine

Cat. # Quantity Price Lead time
1G4E0 1 mg $110 in stock
2G4E0 5 mg $290 in stock
3G4E0 10 mg $450 in stock
4G4E0 25 mg $690 in stock
5G4E0 50 mg $1270 in stock
6G4E0 100 mg $1990 in stock
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Because of its relatively long excited-state lifetime (about 5 nanoseconds), BDP 576/589 dye can be used in various methods based on measuring fluorescence lifetime. Similarly to other dyes of the BDP family, BDP 576/589 has strong hydrophobic properties and is suitable for labeling non-polar and lypophilic biomolecules and their subsequent visualization by fluorescent microscopy, including two-photon microscopy.

This reagent is a tetrazine derivative that can be conjugated with various strained dienophiles such as trans-cyclooctenes and cyclopropenes. This reaction (TCO ligation) is considered to be one of the best bioconjugation reactions because it is very fast and selective in physiological conditions and does not require additional catalyzers, so it is not toxic in vitro and in vivo.

BDP 576/589 absorbance and emission spectra

BDP 576/589 absorbance and emission spectra

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BDP 576/589 carboxylic acid

Derivative of BDP 576/589 fluorophore with non-activated free carboxylic acid group.
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BDP 558/568 tetrazine

Stable fluorophore that can be conjugated with trans-cyclooctene derivatives of biomolecules for their visualization.

Cyanine3.5 carboxylic acid

Unactivated monofunctional Cy3.5 carboxylic acid.

General properties

Appearance: dark colored solid
Mass spec M+ increment: 501.16
Molecular weight: 529.17
Molecular formula: C26H23N8BF2O
Solubility: good in polar organic solvents
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 580
ε, L⋅mol−1⋅cm−1: 98000
Emission maximum, nm: 592
Fluorescence quantum yield: 0.13
CF260: 0.32
CF280: 0.35
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