Annexin V-AF 488 conjugate
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11515 | 1 ug | – | in stock | |
| 21515 | 5 ug | $119 | in stock |
Annexin V (or Annexin A5) belongs to the phospholipid-binding Annexin family of intracellular proteins. In flow cytometry and fluorescent microscopy, Annexin V is commonly used to detect apoptotic cells by its ability to specifically bind to phosphatidylserine, which migrates from the inner to the outer leaflet of the plasma membrane during the early stages of apoptosis.
This Annexin V is a lyophilized conjugate with AF 488, a bright, photostable green fluorophore with spectral characteristics similar to FITC (absorption max. at 495 nm, emission max. at 519 nm).
Annexin V-AF 488 staining alone does not segregate the populations of apoptotic and necrotic cells. For this, additional staining with nuclear dyes that do not penetrate living cells — with propidium iodide or YODi-3 is required. For this purpose, you can also use our ready-to-use Annexin V-AF 488 apoptosis detection kit.
Dissolve the content of the Annexin V-AF lyophilized conjugate tube in 50 µL (11515) or in 250 µL (21515) of deionized water.
Important! The dissolved conjugate should be stored protected from light at
Absorption and emission spectra of AF 488
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Activated ester for modification of proteins/antibodies/peptides followed by introduction into copper-free click chemistry reaction.General properties
| Appearance: | dark orange solid |
| Solubility: | good in water |
| Storage conditions: | Store at -20 °С 9 months. Transportation: at room temperature for 1 week. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
Product citations
- Fayzullina, D.; Yakushov, S.; Kantserova, K.; Belyaeva, E.; Aniskin, D.; Tsibulnikov, S.; Fayzullina, N.; Kalinin, S.; Romantsova, O.; Timashev, P.S.; Schroeder, B.A.; Ulasov, I.V. Carbonic Anhydrase Inhibitors Induce Ferroptosis through Inhibition of AKT/FTH1 Signaling in Ewing Sarcoma Tumor Cells. Cancers, 2023, 15(21), 5225. doi: 10.3390/cancers15215225
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