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Annexin V-AF 488 Apoptosis Detection Kit, with Propidium Iodide

Annexin V-AF 488 Apoptosis Detection Kit, with Propidium Iodide

Cat. #	Quantity	Price	Lead time	Buy this product
11172	10 assays	–	1 days
21172	50 assays	$215 $169	1 days

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Annexin V (or Annexin A5) is a member of the phospholipid-binding annexin family of intracellular proteins. In flow cytometry and fluorescent microscopy, Annexin V is commonly used to detect apoptotic cells by its ability to specifically bind to phosphatidylserine (PS) in a calcium-dependent manner. The method was first reported by Koopman et al. (1994) [1].

In healthy cells, PS is normally retained in the inner leaflet of the plasma membrane. The early stage of apoptosis is accompanied by a loss of membrane phospholipid asymmetry, resulting in the exposure of PS at the cell's surface. In conjunction with vital dyes such as propidium iodide (PI), fluorochrome-labeled Annexin V can distinguish between healthy (Annexin⁻PI⁻), apoptotic (Annexin⁺PI⁻), and necrotic (Annexin⁺PI⁺) cells [2].

The ready-to-use kit contains all necessary reagents to label apoptotic cells with Annexin V-AF 488 conjugate and PI.

AF 488 is bright, photostable, and hydrophilic sulfonated rhodamine fluorophore. The dye emits in the green channel. The spectrum is similar with FITC. Its absorption max. is at 495 nm, the emission max. is at 519 nm.

PI is a membrane-impermeable DNA stain, making it useful for differentiating necrotic, apoptotic, and healthy cells based on membrane integrity. After DNA binding, the dye emits in the orange-red channel. Its absorption max. is at 535 nm, the emission max. is at 617 nm.

[1] Koopman G. et al. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. 1994. 84(5). P.1415-1420.

[2] Martin S.J. et al. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med. 1995. 182(5). P.1545-1556.

Product in action

Flow Cytometry using Annexin V-AF488 Apoptosis Detection Kit (21172, Lumiprobe)

Fluorescence microscopy (100x) using Annexin V-AF488 Apoptosis Detection Kit (21172, Lumiprobe)

Fluorescence microscopy (20x) using Annexin V-AF488 Apoptosis Detection Kit (21172, Lumiprobe)

Absorption and emission spectra of AF 488 and Propidium Iodide

Ready-to-use kit manual

Annexin V-AF Apoptosis Detection Kits with Propidium Iodide manual

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General properties

Storage conditions:	Transportation: at room temperature for 1 week. Store at -20 °С.
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References

Menchinskaya, E. S.; Chingizova, E. A.; Pislyagin, E. A.; Yurchenko, E. A.; Klimovich, A. A.; Zelepuga, E. A.; Aminin, D. L.; Avilov, S. A.; Silchenko, A. S. Mechanisms of Action of Sea Cucumber Triterpene Glycosides Cucumarioside A0-1 and Djakonovioside A Against Human Triple-Negative Breast Cancer. Marine Drugs, 2024, 22(10), 474. doi: 10.3390/md22100474

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.