DAPI, blue fluorescent nucleic acid stain

Cat. # Quantity Price Lead time
2996-1mL 1 mL, 1 mg/mL in Water $75 in stock
2996-50uL 50 uL, 1 mg/mL in Water –   in stock
2996-1mg 1 mg $30 in stock
2996-10mg 10 mg $110 in stock
Found better price? Let us know and we will propose the way forward!

DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in DNA. It is used extensively in fluorescence microscopy, flow cytometry for chromosome staining and as a nuclear counterstain. DAPI is generally used to stain fixed cells since the dye is membrane impermeant, although the dye can enter live cells when used at high concentrations.

When bound to double-stranded DNA, DAPI exhibits ~20-fold enhancement of fluorescence with an absorption maximum at 358 nm and emission maximum at 461 nm. DAPI also binds to RNA, though it is not as strongly fluorescent. When bound to RNA, its emission spectrum shifts to around 500 nm.

There is a slight fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and GFP. Use spectral unmixing if extremely precise image analysis is required.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting DNA and detecting annealed probes in blotting applications by specifically visualizing the double-stranded complex. Also, DAPI staining is a sensitive and specific detection method for mycoplasma.

Absorption and emission spectra of DAPI

Absorption and emission spectra of DAPI

Customers also purchased with this product

ProteOrange Protein Quantification Reagent, 500×

ProteOrange is a fluorescent protein-sensitive dye used for quantitating purified protein samples. The dye shows minimal variability for different proteins. This reagent is a 500× concentrate of ProteOrange dye.

LumiTracker® Mito JC-1

The cationic carbocyanine dye that is widely used as an indicator of mitochondrial membrane potential to study apoptosis and monitor the health of mitochondria.

LumiMount Fluorescence Mounting Medium

An aqueous anti-fade mounting medium for coverslipping cells and tissues stained with various fluorescent dyes.

General properties

Appearance: yellow solid / solution
Molecular weight: 350.25
CAS number: 28718-90-3
Molecular formula: C16H17Cl2N5
Solubility: good in DMF, DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 355
ε, L⋅mol−1⋅cm−1: 28800
Emission maximum, nm: 460

Product citations

  1. Potapenko, E.Y..; Kashko, N.D.; Knorre, D.A. Spontaneous Mutations in Saccharomyces cerevisiae mtDNA Increase Cell-to-Cell Variation in mtDNA Amount. International Journal of Molecular Sciences, 2023, 24(24), 17413. doi: 10.3390/ijms242417413
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.