PO-TAP-1, blue fluorescent nucleic acid stain

Cat. # Quantity Price Lead time
A3810 50 uL, 5 mM/DMSO $85 in stock
23810 200 uL, 5 mM/DMSO $320 in stock
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PO-TAP-1 (Oxazole Blue Monomer, also known as PO-PRO®-1) is a blue fluorescent carbocyanine monomeric dye. PO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make PO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

The dye is used to detect and count cells and microorganisms, perform nucleic acid sequencing, amplification, and hybridization, and as a temperature sensor.

Excitation and emission spectra of PO-TAP-1 (DNA–dye complex)

Excitation and emission spectra of PO-TAP-1 (DNA–dye complex)

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General properties

Appearance: red solution
Molecular weight: 579.26
CAS number: 157199-56-9
Molecular formula: C20H27I2N3O
IUPAC name: trimethyl-[3-[4-[(E)-(3-methyl-1,3-benzoxazol-2-ylidene)methyl]pyridin-1-ium-1-yl]propyl]azanium;diiodide
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 423 (complex)
Emission maximum, nm: 460 (complex)
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