Sulfo-Cyanine5 amine

Cat. # Quantity Price Lead time
133C0 1 mg $110.00 in stock
233C0 5 mg $290.00 in stock
433C0 25 mg $690.00 in stock
533C0 50 mg $1270.00 in stock
633C0 100 mg $1990.00 in stock

Water soluble amino dye for enzymatic transamination labeling, and other coupling with electrophiles. Cyanine5 is a popular fluorophore which is compatible with different fluorescence measuring instruments.

Sulfo-Cyanine5 derivatives possess good water solubility.

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 absorbance and emission spectra

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Amine derivative of fluorescein (FAM) fluorophore, pure 6-isomer, for the enzymatic transamination labeling, reactions with electrophilic reagents, and reductive amination.

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5® channel.

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

General properties

Appearance: dark blue solid
Molecular weight: 740.98
CAS number: 2183440-44-8
Molecular formula: C38H52N4O7S2
IUPAC name: 3H-​Indolium, 1-​[6-​[(6-​aminohexyl)​amino]​-​6-​oxohexyl]​-​2-​[5-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​3,​3-​dimethyl-​5-​sulfo-​, inner salt
Solubility: moderate in water, well soluble in DMF, DMSO, alcohols
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Schotman, M.J.G.; Peters, M.M.C.; Krijger, G.C.; van Adrichem, I.; de Roos, R.; Bemelmans, J.L.M.; Pouderoijen, M.J.; Rutten, M.G.T.A.; Neef, K.; Chamuleau, S.A.J.; Dankers, P.Y.W. In Vivo Retention Quantification of Supramolecular Hydrogels Engineered for Cardiac Delivery. Advanced Healthcare Materials, 2021, 10(10), e2001987. doi: 10.1002/adhm.202001987
  2. Bos, I.; Timmerman, M.; Sprakel, J. FRET-Based Determination of the Exchange Dynamics of Complex Coacervate Core Micelles. Macromolecules, 2021, 54(1), 398–411. doi: 10.1021/acs.macromol.0c02387
  3. Mahmoud, A.M.; Morrow, J.P.; Pizzi, D.; Azizah, A.M.; Davis, T.P.; Tabor, R.F.; Kempe, K. Tuning Cellular Interactions of Carboxylic Acid Side Chain Containing Polyacrylates: The Role of Cyanine Dye Label and Side Chain Type. Biomacromolecules, 2020, 21(8), 3007–3016. doi: 10.1021/acs.biomac.0c00244
  4. Zhou, L.; Wu, Y.; Luo, Y.; Li, H.; Meng, X.; Liu, Cl; Xiang, J.; Zhang, P.; Gong, P.; Cai, L. Mitochondria-localized Self-reporting Small Molecule Decorated Theranostic Agents for Cancer-organelle Transporting and Imaging. ACS Applied Bio Materials, 2019, 2(11), 5164–5173. doi: 10.1021/acsabm.9b00811
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