Perylene azide

Cat. # Quantity Price Lead time
A5530 1 mg $110.00 in stock
B5530 5 mg $210.00 in stock
C5530 10 mg $310.00 in stock
D5530 25 mg $410.00 in stock
E5530 50 mg $695.00 in stock

Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with quantum yield approaching quantitative.

Due to low life time of fluorescence, this probe does not form excimers.

Perylene labeling of biomolecules is used to be difficult and tedious because of low solubility of perylene, and low availability of activated derivatives. However, labeling via Click Chemistry with this perylene azide is nearly as easy, as with other dyes. Labeling peptide, DNA, protein with this polyaromatic hydrocarbon has never been so simple yet.

To increase hydrophilicity, and facilitate labeling, this PAH azide contains hydrophilic triethyleneglycol linker.

Perylene absorption and emission spectra

Perylene absorption and emission spectra

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Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.

THPTA ligand

THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.

General properties

Appearance: orange-yellow solid
Molecular weight: 452.50
CAS number: 1807503-81-6
Molecular formula: C27H24N4O3
IUPAC name: 3-(8-Azido-3,6-trioxaoctylaminocarbonyl)perylene
Solubility: good in dichloromethane and chloroform, moderate in DMSO, DMF, and acetonitrile
Quality control: NMR 1H (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 435; 408; 252
ε, L⋅mol−1⋅cm−1: 36000
Emission maximum, nm: 439; 467
Fluorescence quantum yield: 1.0

Product citations

  1. Westergaard Mulberg, M.; Taskova, M.; Thomsen, R.P.; Okholm, A.H.; Kjems, J.; Astakhova, K. New Fluorescent Nanoparticles for Ultrasensitive Detection of Nucleic Acids by Optical Methods. Chembiochem, 2017, 18(16), 1599–1603. doi: 10.1002/cbic.201700125
  2. Okholm, A.; Kjems, J.; Astakhova, K. Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes. RSC Advances, 2014, 4(86), 45653–45656. doi: 10.1039/c4ra07165d
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