Cyanine3.5 DBCO

Cat. # Quantity Price Lead time
A20F0 1 mg $110.00 in stock
B20F0 5 mg $210.00 in stock
C20F0 10 mg $310.00 in stock
D20F0 25 mg $410.00 in stock
E20F0 50 mg $695.00 in stock
F20F0 100 mg $1190.00 in stock

Cyanine3.5 is a bright and photostable fluorophore, which is similar in its spectral characteristics to Cy3.5™ dye. Cyanine3.5 DBCO fluorescence maximum is at 604 nm and in the orange-red spectrum range.

Cyanine3.5 fluorescence is pH insensitive in the range from 4 to 10 and can be detected in the spectrum range with a low level of autofluorescence of biological samples.

Cyanine3.5 DBCO (dibenzocyclooctyne) easily forms conjugates with azide derivatives of biomolecules in copper-free Click Chemistry. The process runs without catalysts or increased temperature, resulting in stable triazoles.

Conjugates of biomolecules with Cyanine3.5 can be used for various microscopy assays, including FRET-microscopy, and in proteomics.

Absorption and emission spectra of Cyanine3.5

Absorption and emission spectra of Cyanine3.5

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General properties

Appearance: dark violet solid
Molecular weight: 944.95
Molecular formula: C59H61N4BF4O2
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22
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