Cyanine5 tetrazine

Tetrazines Cyanine5 Copper-free click chemistry
Cat. # Quantity Price Lead time
130E0 1 mg $125 in stock
230E0 5 mg $260 in stock
430E0 25 mg $510 in stock
530E0 50 mg $895 in stock
630E0 100 mg $1490 in stock
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Tetrazine-cyclooctene (TCO) ligation is one of the fastest bioconjugation reactions known to date. In order to use it for fluorescent labeling, a fluorescent reporter molecule bearing one of the reacting groups is necessary.

Cyanine5 tetrazine is a molecule bearing Cyanine5 fluorophore, and tetrazine moiety. Cyanine5 is a very popular fluorophore compatible with numerous instruments.

Cyanine5 tetrazine, 630E0
Cyanine5 tetrazine, 630E0
Cyanine5 tetrazine, 630E0
Structure of Cyanine5 tetrazine
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Absorption and emission spectra of Cyanine5

Absorption and emission spectra of Cyanine5

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Cyanine5 azide is a reagent for click chemistry labeling with Cyanine5, a popular fluorescent dye.
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FAM tetrazine, 6-isomer

This molecule is a conjugate of bright fluorophore FAM (fluorescein) and tetrazine, a reactive group that reacts with trans-cycloalkenes in a reaction called IEDDA (inverse electron demand Diels-Alder reaction).

General properties

Appearance: dark blue solid
Mass spec M+ increment: 637.4
Molecular weight: 811.84
Molecular formula: C42H48N7F6OP
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Santiana, J.J. ; Sawant, S.S.; Gomez, N.; Rouge, J. L. Multi-Layered Stimuli Responsive DNA Micelles for the Stepwise Controlled Release of Small Molecules. Journal of Materials Chemistry B, 2022, 10(37), 7518–7526. doi: 10.1039/D1TB02722K
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