AF 488 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| S1830 | 250 ug | – | in stock | |
| 11830 | 1 mg | $125 | in stock | |
| 21830 | 5 mg | $325 | in stock | |
| 41830 | 25 mg |
$950
|
in stock | |
| 51830 | 50 mg |
$1625
|
in stock | |
| 61830 | 100 mg |
$2090
|
in stock |
AF 488 is a fluorescent dye. AF 488 is a fluorophore pH-insensitive over a broad pH range (from 4 to 10). It has an absorption maximum at 495 nm and an emission maximum at 519 nm in the green spectrum region. The dye is hydrophilic and can be used to introduce the fluorescent label into various molecules, including proteins and antibodies. Conjugates of molecules with AF 488 have high brightness and photostability and are commonly used in flow cytometry and microscopy. This allows the detection of biological objects with high sensitivity at a longer imaging time.
AF 488 azide interacts with alkynyl derivatives of biomolecules in Click Chemistry reactions either in the presence of copper (I) catalyst (with terminal alkynes) or without catalyst (with cyclooctynes), leading to the formation of stable adducts.
Absorption and emission spectra of AF 488
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Biotin alkyne is a reactive affinity label which can be attached to biomolecules via click reaction.General properties
| Appearance: | orange solid |
| Molecular weight: | 692.76 |
| CAS number: | 1679326-36-3 (with azidohexyl group) |
| Molecular formula: | C24H18K2N6O10S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
Product citations
- Baleva, M.V.; Piunova, U.; Chicherin, I.; Vasilev, R.; Levitskii, S.; Kamenski, P. Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells. International Journal of Molecular Sciences, 2024, 25(1), 93. doi: 10.3390/ijms25010093
- Ivanova, A.; Gruzova, O.; Ermolaeva, E.; Astakhova, O.; Itaman, S.; Enikolopov, G.; Lazutkin, A. Synthetic Thymidine Analog Labeling without Misconceptions. Cells, 2022, 11(12), 1888. doi: 10.3390/cells11121888
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