sulfo-Cyanine5.5 NHS ester

Cat. # Quantity Price Lead time
17320 1 mg $125 in stock
27320 5 mg $325 5 days
47320 25 mg $850 in stock
57320 50 mg $1490 in stock
67320 100 mg $1990 in stock
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sulfo-Cyanine5.5 NHS ester is an amine-reactive activated ester of sulfonated far-red Cyanine5.5 fluorophore, an analog of Cy5.5®. This is a reagent of choice for the labeling of antibodies, sensitive proteins, and others which require reactions in a purely aqueous environment or without any significant additions of organic co-solvents.

The dye is well-suitable for non-invasive in vivo NIR imaging and applications requiring low fluorescent background.

Absorption and emission spectra of sulfo-Cyanine5.5

Absorption and emission spectra of sulfo-Cyanine5.5

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General properties

Appearance: dark blue solid
Mass spec M+ increment: 884.15
Molecular weight: 1114.37
CAS number: 2419286-92-1
Molecular formula: C44H42N3K3O16S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 675
ε, L⋅mol−1⋅cm−1: 211000
Emission maximum, nm: 694
Fluorescence quantum yield: 0.21
CF260: 0.09
CF280: 0.11

Product citations

  1. Yao, P.; Liu, R.; Broginni, T.; Thunemann, M.; Kleinfeld, D. Guide to the Construction and Use of an Adaptive Optics Two-Photon Microscope with Direct Wavefront Sensing. bioRxiv, 2023, preprint. doi: 10.1101/2023.01.24.525307
  2. Zhou, L.; He, F.; Xiang, X.; Dong, C.; Xiang, T.; Li, X.; Li, H.; Bu, L.; Wang, Y.; Ma, X. Radioactive and Fluorescent Dual Modality Cysteine Cathepsin B Activity-Based Probe for Cancer Theranostics. Mol. Pharmaceutics, 2023, 20(7), 3539–3548. doi: 10.1021/acs.molpharmaceut.3c00148
  3. Shevchuk, A. I.; Svinko, V. O.; Smirnov, A. N.; Solovyeva, E. V. SERS Study of Cyanine Dyes: Optimization of Metal Core and Molecular Label Choice for Plasmonic Nanotags. Dyes and Pigments, 2023, 216, 111329. doi: 10.1016/j.dyepig.2023.111329.
  4. Svinko, V. O.; Smirnov, A. N.; Shevchuk, A. I.; Demenshin, A. I.; Smirnov, A. A.; Solovyeva, E. V. Comparative Study of Fluorescence Core-Shell Nanotags with Different Morphology of Gold Core. Colloids and Surfaces B: Biointerfaces, 2023, 226, 113306. doi: 10.1016/j.colsurfb.2023.113306
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