Sulfo-Cyanine3 maleimide

Cat. # Quantity Price Lead time
11380 1 mg $110.00 in stock
21380 5 mg $290.00 in stock
41380 25 mg $690.00 in stock
51380 50 mg $1270.00 in stock
61380 100 mg $1990.00 in stock

Sulfo-Cyanine3 maleimide is a water soluble, thiol reactive dye for the labeling with hydrophilic sulfo-Cyanine3 fluorophore. This is an analog of Cy3® maleimide.

This product is recommended for the labeling of antibodies and other labile proteins in mild, purely aqueous conditions. The dye is water soluble and does not require use of organic co-solvent.

A non-sulfonated Cyanine3 maleimide is available.

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide

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Cyanine5.5 carboxylic acid

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General properties

Appearance: red powder
Molecular weight: 776.96
CAS number: 1656990-68-9
Molecular formula: C36H41KN4O9S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​[6-​[[2-​(2,​5-​dihydro-​2,​5-​dioxo-​1H-​pyrrol-​1-​yl)​ethyl]​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt, potassium salt
Solubility: soluble in water (0.39 M = 30 g/L), DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

Product citations

  1. Comerci, C.J.; Herrmann, J.; Yoon, J.; Jabbarpour, F.; Zhou, X.; Nomellini, J.F.; Smit, J.; Shapiro, L.; Wakatsuki, S.; Moerner, W.E. Topologically-guided continuous protein crystallization controls bacterial surface layer self-assembly. Nature Communications, 2019, 10(1), 2731. doi: 10.1038/s41467-019-10650-x
  2. Tischer, D.K.; Weiner, O.D. Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling. eLIFE, 2019, 8, e42489. doi: 10.7554/eLife.42498
  3. Ni, Y.; Arts, R.; Merkx, M. Ratiometric bioluminescent sensor proteins based on intramolecular split luciferase complementation. ACS Sensors, 2019, 4(1), 20–25. doi: 10.1021/acssensors.8b01381
  4. Agrawalla, B.K.; Wang, T.; Riegger, A.; Domogalla, M.P.; Steinbrink, K.; Dörfler, T.; Chen, X.; Boldt, F.; Lamla, M.; Michaelis, J.; Kuan, S.L.; Weil, T. Chemoselective Dual and Triple Labelling of Native and Recombinant Proteins. Bioconjugate Chemistry, 2018, 29(1), 29–34. doi: 10.1021/acs.bioconjchem.7b00675
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