Cyanine3 maleimide

Cat. # Quantity Price Lead time
11080 1 mg $110.00 in stock
21080 5 mg $210.00 5 days
41080 25 mg $410.00 in stock
51080 50 mg $695.00 in stock
61080 100 mg $1190.00 in stock

Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).

Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.

Labeling with Cyanine3 maleimide is selective, and efficient.

We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.

Cy3 absorbance and emission spectra

Cy3 absorbance and emission spectra

Customers also purchased with this product

Sulfo-Cyanine3 tetrazine

Water soluble sulfo-Cyanine3 dye tetrazine derivative for TCO ligation labeling with trans-cyclooctenes.

Pyrenebutyric acid NHS ester

Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

General properties

Appearance: red powder
Molecular weight: 666.56
CAS number: 1593644-29-1 (without anion)
Molecular formula: C36H43N4O3BF4
Solubility: well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
  2. Budiardjo, S.J.; Deay, J.J.; Calkins, A.L.; Wimalasena, V.K.; Montezano, D.; Biteen, J.S.; Slusky, J.S.G. Colicin E1 Fragments Potentiate Antibiotics by Plugging TolC. bioRxiv, preprint. doi: 10.1101/692251
  3. Fliervoet, L.A.L.; Lisitsyna, E.S.; Durandin, N.A.; Kotsis, I.; Maas-Bakker, R.F.M.; Yliperttula, M.; Hennink, W.E.; Vuorimaa-Laukkanen, E.; Vermonden, T. Structure and dynamics of thermosensitive pDNA polyplexes studied by time-resolved fluorescence spectroscopy. Biomacromolecules, 2020, 21(1), 73–88. doi: 10.1021/acs.biomac.9b00896
  4. Liu, J.; Lee, R.; Britton, B.M.; London, J.A.; Yang, K.; Hanne, J.; Lee, J.-B.; Fishel, R. MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair. Nature Communications, 2019. doi: 10.1038/s41467-019-13191-5
Show more (24)
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.