Cyanine3 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11080 | 1 mg | $110.00 | in stock | |
| 21080 | 5 mg | $210.00 | in stock | |
| 41080 | 25 mg |
$410.00
|
in stock | |
| 51080 | 50 mg |
$695.00
|
in stock | |
| 61080 | 100 mg |
$1190.00
|
in stock |
Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
Labeling with Cyanine3 maleimide is selective, and efficient.
We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.
Cy3 absorbance and emission spectra
Recommended protocol
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Sulfo-Cyanine7 azide
Water-soluble NIR fluorescent dye azide for Click chemistryGeneral properties
| Appearance: | red powder |
| Molecular weight: | 666.56 |
| CAS number: | 1593644-29-1 (without anion) |
| Molecular formula: | C36H43N4O3BF4 |
| Solubility: | well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Fliervoet, L.A.L.; Lisitsyna, E.S.; Durandin, N.A.; Kotsis, I.; Maas-Bakker, R.F.M.; Yliperttula, M.; Hennink, W.E.; Vuorimaa-Laukkanen, E.; Vermonden, T. Structure and dynamics of thermosensitive pDNA polyplexes studied by time-resolved fluorescence spectroscopy. Biomacromolecules, in press. doi: 10.1021/acs.biomac.9b00896
- Budiardjo, S.J.; Deay, J.J.; Calkins, A.L.; Wimalasena, V.K.; Montezano, D.; Biteen, J.S.; Slusky, J.S.G. Colicin E1 Fragments Potentiate Antibiotics by Plugging TolC. bioRxiv, preprint. doi: 10.1101/692251
- Catipovic, M.A.; Bauer, B.W.; Loparo, J.J.; Rapoport, T.A. Protein translocation by the SecA ATPase occurs by a power-stroke mechanism. EMBO Journal, 2019, 38(9), e101140. doi: 10.15252/embj.2018101140
- Armache, J.P.; Gamarra, N.; Johnson, S.L.; Leonard, J.D.; Wu, S.; Narlikar, G.J.; Cheng, Y. Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome. eLife, 2019, 8, e46057. doi: 10.7554/eLife.46057
Cy® is a trademark of GE Healthcare.
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