Cyanine3 maleimide

Cat. # Quantity Price Lead time
11080 1 mg $110.00 in stock
21080 5 mg $210.00 in stock
41080 25 mg $410.00 in stock
51080 50 mg $695.00 in stock
61080 100 mg $1190.00 in stock

Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).

Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.

Labeling with Cyanine3 maleimide is selective, and efficient.

We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.

Cy3 absorbance and emission spectra

Cy3 absorbance and emission spectra

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General properties

Appearance: red powder
Molecular weight: 666.56
Molecular formula: C36H43N4O3BF4
Solubility: well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Singh, R.K.; Fan, J.; Gioacchini, N.; Watanabe, S.; Bilsel, O.; Peterson, C.L. Transient Kinetic Analysis of SWR1C-Catalyzed H2A.Z Deposition Unravels the Impact of Nucleosome Dynamics and the Asymmetry of Histone Exchange. Cell Reports, 2019, 27, 374–386.e4. doi: 10.1016/j.celrep.2019.03.035
  2. Gagni, P.; Romanato, A.; Bergamaschi, G.; Bettotti, P.; Vanna, R.; Piotto, C.; Morasso, C.F.; Chiari, M.; Cretich, M.; Gori, A. A self-assembling peptide hydrogel for ultrarapid 3D bioassays. Nanoscale Advances, 2019, 1(2), 490–497. doi: 10.1039/c8na00158h
  3. Wu, B.; Zhang, H.; Sun, R.; Peng, S.; Cooperman, B.S.; Goldman, Y.E.; Chen, C. Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots. Nucleic Acids Research, 2018, 46(18), 9736–9748. doi: 10.1093/nar/gky636
  4. Liu, G.W.; Prossnitz, A.N.; Eng, D.G.; Cheng, Y.; Subrahmanyam, N.; Pippin, J.W.; Lamm, R.J.; Ngambenjawong, C.; Ghandehari, H.; Shankland, S.J.; Pun, S.H. Glomerular disease augments kidney accumulation of synthetic anionic polymers. Biomaterials, 2018, 178, 317–325. doi: 10.1016/j.biomaterials.2018.06.001
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