Cyanine3 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11080 | 1 mg | $110.00 | in stock | |
| 21080 | 5 mg | $210.00 | in stock | |
| 41080 | 25 mg |
$410.00
|
in stock | |
| 51080 | 50 mg |
$695.00
|
in stock | |
| 61080 | 100 mg |
$1190.00
|
in stock |
Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
Labeling with Cyanine3 maleimide is selective, and efficient.
We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.
Cy3 absorbance and emission spectra
Recommended protocol
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Sulfo-Cyanine3 tetrazine
Water soluble sulfo-Cyanine3 dye tetrazine derivative for TCO ligation labeling with trans-cyclooctenes.BDP TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.DMF (dimethylformamide), labeling grade
Dry high purity amine free solvent for NHS ester labeling.General properties
| Appearance: | red powder |
| Molecular weight: | 666.56 |
| CAS number: | 1593644-29-1 (without anion) |
| Molecular formula: | C36H43N4O3BF4 |
| Solubility: | well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
- Fliervoet, L.A.L.; Lisitsyna, E.S.; Durandin, N.A.; Kotsis, I.; Maas-Bakker, R.F.M.; Yliperttula, M.; Hennink, W.E.; Vuorimaa-Laukkanen, E.; Vermonden, T. Structure and dynamics of thermosensitive pDNA polyplexes studied by time-resolved fluorescence spectroscopy. Biomacromolecules, in press. doi: 10.1021/acs.biomac.9b00896
- Budiardjo, S.J.; Deay, J.J.; Calkins, A.L.; Wimalasena, V.K.; Montezano, D.; Biteen, J.S.; Slusky, J.S.G. Colicin E1 Fragments Potentiate Antibiotics by Plugging TolC. bioRxiv, preprint. doi: 10.1101/692251
- Liu, J.; Lee, R.; Britton, B.M.; London, J.A.; Yang, K.; Hanne, J.; Lee, J.-B.; Fishel, R. MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair. Nature Communications, 2019. doi: 10.1038/s41467-019-13191-5
Cy® is a trademark of GE Healthcare.
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