Cyanine3 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11080 | 1 mg | $125.00 | in stock | |
| 21080 | 5 mg | $260.00 | in stock | |
| 41080 | 25 mg |
$510.00
|
in stock | |
| 51080 | 50 mg |
$895.00
|
in stock | |
| 61080 | 100 mg |
$1490.00
|
in stock |
Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
Labeling with Cyanine3 maleimide is selective, and efficient.
We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.
Cy3 absorbance and emission spectra
Recommended protocol
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sulfo-Cyanine7.5 maleimide
sulfo-Cyanine7.5 is a highly hydrophilic near infrared dye for in vivo imaging. This maleimide derivative is useful for the labeling of thiol groups of proteins and peptides.sulfo-Cyanine3 dUTP
Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3. The nucleotide contains a long linker that allows to incorporate the dye efficiently into DNA strand by various enzymes including Taq polymerase.sulfo-Cyanine5 alkyne
sulfo-Cyanine5 is a water-soluble Cyanine5 dye alkyne for click chemistry.General properties
| Appearance: | red powder |
| Molecular weight: | 666.56 |
| Molecular formula: | C36H43N4O3BF4 |
| Solubility: | well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Chadda, R.; Lee, T.; Sandal, P.; Mahoney-Kruszka, R.; Robertson, J. L. A Thermodynamic Analysis of CLC Transporter Dimerization in Lipid Bilayers. bioRxiv, 2023, preprint. doi: 10.1101/2023.03.14.532678
- Song, Q.-X.; Liu, N.-N.; Liu, Z.-X.; Zhang, Y.-Z.; Rety, S.; Hou, X.-M.; Xi, X.-G. Nonstructural N- and C-Tails of Dbp2 Confer the Protein Full Helicase Activities. Journal of Biological Chemistry, 2023, 299(5), 104592. doi: 10.1016/j.jbc.2023.104592
- Naeimipour, S.; Boroojeni, F. R.; Lifwergren, P.; Selegård, R.; Aili, D. Multimodal andDynamic Cross-Linking of Modular Thiolated Alginate-Based Bioinks. Materials Today Advances, 2023, 19, 100415. doi: 10.1016/j.mtadv.2023.100415
- Naeimipour, S.; Rasti Boroojeni, F.; Selegård, R.; Aili, D. Enzymatically Triggered Deprotection and Cross-Linking of Thiolated Alginate-Based Bioinks. Chem. Mater., 2022, 34(21), 9536–9545. doi: 10.1021/acs.chemmater.2c02037
Cy® is a trademark of GE Healthcare.
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