Cyanine3 maleimide

Cat. # Quantity Price Lead time
11080 1 mg $110.00 in stock
21080 5 mg $210.00 in stock
41080 25 mg $410.00 in stock
51080 50 mg $695.00 in stock
61080 100 mg $1190.00 in stock

Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).

Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.

Labeling with Cyanine3 maleimide is selective, and efficient.

We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.

Cy3 absorbance and emission spectra

Cy3 absorbance and emission spectra

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EDC, coupling grade

Water soluble carbodiimide for the coupling of carboxylic acids with amines.

Cyanine3.5 carboxylic acid

Unactivated monofunctional Cy3.5 carboxylic acid.

Sulfo-Cyanine5.5 carboxylic acid

Water soluble far red fluorescent dye sulfo-Cyanine5.5, free non-activated carboxylic acid. Useful for the calibration, and as a non-reactive control.

General properties

Appearance: red powder
Molecular weight: 666.56
CAS number: 1593644-29-1 (without anion)
Molecular formula: C36H43N4O3BF4
Solubility: well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
  2. Budiardjo, S.J.; Deay, J.J.; Calkins, A.L.; Wimalasena, V.K.; Montezano, D.; Biteen, J.S.; Slusky, J.S.G. Colicin E1 Fragments Potentiate Antibiotics by Plugging TolC. bioRxiv, preprint. doi: 10.1101/692251
  3. Zhang, N.; Shi, S.; Yoo, B.; Yuan, X.; Li, W.; Zhang, S. 2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications. Journal of Visualized Experiments, 2020, e61281. doi: 10.3791/61281
  4. Fliervoet, L.A.L.; Lisitsyna, E.S.; Durandin, N.A.; Kotsis, I.; Maas-Bakker, R.F.M.; Yliperttula, M.; Hennink, W.E.; Vuorimaa-Laukkanen, E.; Vermonden, T. Structure and dynamics of thermosensitive pDNA polyplexes studied by time-resolved fluorescence spectroscopy. Biomacromolecules, 2020, 21(1), 73–88. doi: 10.1021/acs.biomac.9b00896
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