Cyanine3 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11080 | 1 mg | $110.00 | in stock | |
| 21080 | 5 mg | $210.00 | 5 days | |
| 41080 | 25 mg |
$410.00
|
in stock | |
| 51080 | 50 mg |
$695.00
|
in stock | |
| 61080 | 100 mg |
$1190.00
|
in stock |
Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
Labeling with Cyanine3 maleimide is selective, and efficient.
We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.
Cy3 absorbance and emission spectra
Recommended protocol
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Water soluble sulfo-Cyanine3 dye tetrazine derivative for TCO ligation labeling with trans-cyclooctenes.Pyrenebutyric acid NHS ester
Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.General properties
| Appearance: | red powder |
| Molecular weight: | 666.56 |
| CAS number: | 1593644-29-1 (without anion) |
| Molecular formula: | C36H43N4O3BF4 |
| Solubility: | well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
- Budiardjo, S.J.; Deay, J.J.; Calkins, A.L.; Wimalasena, V.K.; Montezano, D.; Biteen, J.S.; Slusky, J.S.G. Colicin E1 Fragments Potentiate Antibiotics by Plugging TolC. bioRxiv, preprint. doi: 10.1101/692251
- Fliervoet, L.A.L.; Lisitsyna, E.S.; Durandin, N.A.; Kotsis, I.; Maas-Bakker, R.F.M.; Yliperttula, M.; Hennink, W.E.; Vuorimaa-Laukkanen, E.; Vermonden, T. Structure and dynamics of thermosensitive pDNA polyplexes studied by time-resolved fluorescence spectroscopy. Biomacromolecules, 2020, 21(1), 73–88. doi: 10.1021/acs.biomac.9b00896
- Liu, J.; Lee, R.; Britton, B.M.; London, J.A.; Yang, K.; Hanne, J.; Lee, J.-B.; Fishel, R. MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair. Nature Communications, 2019. doi: 10.1038/s41467-019-13191-5
Cy® is a trademark of GE Healthcare.
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