Sulfo-Cyanine3 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A1330 | 1 mg | $110.00 | in stock | |
| B1330 | 5 mg | $290.00 | in stock | |
| C1330 | 10 mg |
$470.00
|
in stock | |
| D1330 | 25 mg |
$690.00
|
in stock | |
| E1330 | 50 mg |
$1270.00
|
in stock | |
| F1330 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine3 azide is a water-soluble dye azide for Click Chemistry. Absorbance and emission of the dye are identical to Cy3® fluorophore.
Brightness and photostability of fluorophore, together with high water solubility, makes this reagent an ideal choice for Click chemistry labeling of various molecules in aqueous phase.
Click reaction can be carried out in pure water without need to use organic co-solvent. The reagent is designed for the labeling of sensitive molecules such as proteins, and even intact biological objects.
Sulfo-Cyanine3 absorbance and emission spectra
Recommended protocol
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Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.Sulfo-Cyanine5.5 azide
Sulfo-Cyanine5.5 azide is a derivative of water soluble far red emitting fluorescent cyanine dye. The azide group can be conjugated by either copper-catalyzed or copper free Click chemistry. The reagent possesses high aqueous solubility and hydrophilicity, and recommended for the labeling of biomolecules in aqueous environment.DBCO NHS ester
Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper free Click chemistry (spAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.General properties
| Appearance: | dark red crystals |
| Molecular weight: | 736.94 |
| CAS number: | 2055138-89-9 (sodium salt), 1658416-54-6 |
| Molecular formula: | C33H41N6KO7S2 |
| Solubility: | soluble in water (0.62 M = 46 g/L), DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Product citations
- Doll, F.; Steimbach, R.; Zumbusch, A. Direct Imaging of Protein-Specific Methylation in Mammalian Cells. Chembiochem, 2019, 20(10), 1315–1325. doi: 10.1002/cbic.201800787
- Cohen, J.; Raviv, S.; Adir, O.; Padmanabhan, K.; Soffer, A.; Luxenburg, C. The Wave complex controls epidermal morphogenesis and proliferation by suppressing Wnt-Sox9 signaling. Journal of Cell Biology, 2019, 218(4), 1390–1406. doi: 10.1083/jcb.201807216
- Melnychuk, N.; Klymchenko, A.S. DNA-Functionalized Dye-Loaded Polymeric Nanoparticles: Ultrabright FRET Platform for Amplified Detection of Nucleic Acids. Journal of the American Chemical Society, 2018, 140(34), 10856–10865. doi: 10.1021/jacs.8b05840
- Hernandez-Segura, A.; Brandenburg, S.; Demaria, M. Induction and Validation of Cellular Senescence in Primary Human Cells. Journal of Visualized Experiments, 2018, 136, e57782. doi: 10.3791/57782
Cy® is a trademark of GE Healthcare.
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