Sulfo-Cyanine3 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A1330 | 1 mg | $110.00 | in stock | |
| B1330 | 5 mg | $290.00 | in stock | |
| C1330 | 10 mg |
$470.00
|
in stock | |
| D1330 | 25 mg |
$690.00
|
in stock | |
| E1330 | 50 mg |
$1270.00
|
in stock | |
| F1330 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine3 azide is a water-soluble dye azide for Click Chemistry. Absorbance and emission of the dye are identical to Cy3® fluorophore.
Brightness and photostability of fluorophore, together with high water solubility, makes this reagent an ideal choice for Click chemistry labeling of various molecules in aqueous phase.
Click reaction can be carried out in pure water without need to use organic co-solvent. The reagent is designed for the labeling of sensitive molecules such as proteins, and even intact biological objects.
Sulfo-Cyanine3 absorbance and emission spectra
Recommended protocol
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1-Ethynyl pyrene
Pyrene is polyaromatic hydrocarbon containing four fused rings. This reagent contains terminal alkyne group for Click Chemistry and other reactions, such as Sonogashira coupling.Cyanine5.5 amine
Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.
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FAM alkyne, 6-isomer
Fluorescein dye alkyne for Click Chemistry, pure 6-isomer.General properties
| Appearance: | dark red crystals |
| Molecular weight: | 736.94 |
| CAS number: | 2055138-89-9 (sodium salt), 1658416-54-6 |
| Molecular formula: | C33H41N6KO7S2 |
| Solubility: | soluble in water (0.62 M = 46 g/L), DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Product citations
- Sharma, P.; Mesci, P.; Carromeu, C.; McClatchy, D.R.; Schiapparelli, L.; Yates, J.R.; Muotri, A.R.; Cline, H.T. Exosomes regulate neurogenesis and circuit assembly. Proceedings of the National Academy of Sciences of the U.S.A., in press. doi: 10.1073/pnas.1902513116
- Antonov, S.A.; Novosadova, E.V.; Kobylansky, A.G.; Tarantul, V.Z.; Grivennikov, I.A. A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining. Journal of Histochemistry & Cytochemistry, in press. doi: 10.1369/0022155419864113
- Doll, F.; Steimbach, R.; Zumbusch, A. Direct Imaging of Protein-Specific Methylation in Mammalian Cells. Chembiochem, 2019, 20(10), 1315–1325. doi: 10.1002/cbic.201800787
- Cohen, J.; Raviv, S.; Adir, O.; Padmanabhan, K.; Soffer, A.; Luxenburg, C. The Wave complex controls epidermal morphogenesis and proliferation by suppressing Wnt-Sox9 signaling. Journal of Cell Biology, 2019, 218(4), 1390–1406. doi: 10.1083/jcb.201807216
Cy® is a trademark of GE Healthcare.
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