Sulfo-Cyanine3 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A1330 | 1 mg | $110.00 | in stock | |
| B1330 | 5 mg | $290.00 | in stock | |
| C1330 | 10 mg |
$470.00
|
in stock | |
| D1330 | 25 mg |
$690.00
|
in stock | |
| E1330 | 50 mg |
$1270.00
|
in stock | |
| F1330 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine3 azide is a water-soluble dye azide for Click Chemistry. Absorbance and emission of the dye are identical to Cy3® fluorophore.
Brightness and photostability of fluorophore, together with high water solubility, makes this reagent an ideal choice for Click chemistry labeling of various molecules in aqueous phase.
Click reaction can be carried out in pure water without need to use organic co-solvent. The reagent is designed for the labeling of sensitive molecules such as proteins, and even intact biological objects.
Sulfo-Cyanine3 absorbance and emission spectra
Recommended protocol
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General properties
| Appearance: | dark red crystals |
| Molecular weight: | 736.94 |
| CAS number: | 2055138-89-9 (sodium salt), 1658416-54-6 |
| Molecular formula: | C33H41N6KO7S2 |
| Solubility: | very good in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Product citations
- Doll, F.; Steimbach, R.; Zumbusch, A. Direct Imaging of Protein-Specific Methylation in Mammalian Cells. Chembiochem, 2019, 20(10), 1315–1325. doi: 10.1002/cbic.201800787
- Cohen, J.; Raviv, S.; Adir, O.; Padmanabhan, K.; Soffer, A.; Luxenburg, C. The Wave complex controls epidermal morphogenesis and proliferation by suppressing Wnt-Sox9 signaling. Journal of Cell Biology, 2019, 218(4), 1390–1406. doi: 10.1083/jcb.201807216
- Melnychuk, N.; Klymchenko, A.S. DNA-Functionalized Dye-Loaded Polymeric Nanoparticles: Ultrabright FRET Platform for Amplified Detection of Nucleic Acids. Journal of the American Chemical Society, 2018, 140(34), 10856–10865. doi: 10.1021/jacs.8b05840
- Hernandez-Segura, A.; Brandenburg, S.; Demaria, M. Induction and Validation of Cellular Senescence in Primary Human Cells. Journal of Visualized Experiments, 2018, 136, e57782. doi: 10.3791/57782
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