HEX azide, 6-isomer

Cat. # Quantity Price Lead time
AB230 1 mg $110 in stock
BB230 5 mg $165 in stock
CB230 10 mg $210 in stock
DB230 25 mg $285 in stock
EB230 50 mg $495 in stock
FB230 100 mg $880 in stock
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Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR.

Oligonucleotides with a HEX label can be easily generated using HEX azide and alkyne-containing oligonucleotide via azide-alkyne cycloaddition.

HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in green-yellow area, and it can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of the similar spectral characteristics.

Absorption and emission spectra of HEX

Absorption and emission spectra of HEX

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ROX reference dye for qPCR

ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.

JOE azide, 5-isomer

JOE is a chlorinated fluorescein dye which is often used for HEX channel for oligonucleotides. It is less hydrophobic than HEX. This is an azide derivative for сlick chemistry

General properties

Appearance: orange solid
Mass spec M+ increment: 661.9
Molecular weight: 665.09
CAS number: 1450752-91-6
Molecular formula: C24H12N4Cl6O6
IUPAC name: N-(3-azidopropyl)-2',4,4',5',7,7'-hexachloro-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxamide
Solubility: very soluble in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 87770
Emission maximum, nm: 549
Fluorescence quantum yield: 0.57
CF260: 0.30
CF280: 0.13
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