HEX azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| AB230 | 1 mg | $110.00 | in stock | |
| BB230 | 5 mg | $210.00 | in stock | |
| CB230 | 10 mg | $310.00 | in stock | |
| DB230 | 25 mg |
$410.00
|
in stock | |
| EB230 | 50 mg |
$695.00
|
in stock | |
| FB230 | 100 mg |
$1190.00
|
in stock |
Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR.
Oligonucleotides with a HEX label can be easily generated using HEX azide and alkyne-containing oligonucleotide via azide-alkyne cycloaddition.
HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in green-yellow area, and it can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of the similar spectral characteristics.
Absorption and emission spectra of HEX
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Amine derivative of fluorescein (FAM) fluorophore, pure 6-isomer, for the enzymatic transamination labeling, reactions with electrophilic reagents, and reductive amination.General properties
| Appearance: | orange solid |
| Mass spec M+ increment: | 661.9 |
| Molecular weight: | 665.09 |
| CAS number: | 1450752-91-6 |
| Molecular formula: | C24H12N4Cl6O6 |
| IUPAC name: | N-(3-azidopropyl)-2',4,4',5',7,7'-hexachloro-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxamide |
| Solubility: | very soluble in DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 87770 |
| Emission maximum, nm: | 549 |
| Fluorescence quantum yield: | 0.57 |
| CF260: | 0.30 |
| CF280: | 0.13 |
BODIPY™, VIC™ are trademarks of Thermo Fisher Scientific.
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