FAM amine, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 151C0 | 1 mg | $110.00 | in stock | |
| 251C0 | 5 mg | $210.00 | in stock | |
| 451C0 | 25 mg |
$410.00
|
in stock | |
| 551C0 | 50 mg |
$695.00
|
in stock | |
| 651C0 | 100 mg |
$1190.00
|
in stock |
Fluorescein derivative with amine group, contains pure 6-isomer of the fluorophore. This reagent can be used for the modification of biomolecules by enzymatic transamination. Its aliphatic amine groups also reacts with electrophiles (like activated esters). This amine can be also conjugated with carbonyl compounds (aldehydes and ketones) by means of reductive amination.
Absorption and emission spectra of FAM
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Near infrared dye Cyanine7.5 with free amino group.Coumarin 343 X azide
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.General properties
| Appearance: | yellow solid |
| Molecular weight: | 510.97 |
| CAS number: | 2183440-42-6 (hydrochloride), 1313393-44-0 |
| Molecular formula: | C27H27N2ClO6 |
| IUPAC name: | 5-aminohexylaminocarbonylfluorescein hydrochloride |
| Solubility: | good in methanol, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 490 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 513 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.20 |
| CF280: | 0.17 |
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