FAM amine, 6-isomer

Cat. # Quantity Price Lead time
151C0 1 mg $110.00 in stock
251C0 5 mg $210.00 in stock
451C0 25 mg $410.00 in stock
551C0 50 mg $695.00 in stock
651C0 100 mg $1190.00 in stock

Fluorescein derivative with amine group, contains pure 6-isomer of the fluorophore. This reagent can be used for the modification of biomolecules by enzymatic transamination. Its aliphatic amine groups also reacts with electrophiles (like activated esters). This amine can be also conjugated with carbonyl compounds (aldehydes and ketones) by means of reductive amination.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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FAM amine, 5-isomer

Amino derivative of fluorescein, pure 5-isomer. Contains a linker arm between the aliphatic amine group, and the fluorophore. Useful for the labeling via enzymatic transamination, for the reactions with electrophilic reagents, and for reductive amination.

General properties

Appearance: yellow solid
Molecular weight: 510.97
Molecular formula: C27H27N2ClO6
IUPAC name: 5-aminohexylaminocarbonylfluorescein hydrochloride
Solubility: good in methanol, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17
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