FAM hydrazide, 6-isomer

Cat. # Quantity Price Lead time
15170 1 mg $110 in stock
25170 5 mg $210 in stock
45170 25 mg $410 in stock
55170 50 mg $695 in stock
65170 100 mg $1190 in stock
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Fluorescein (FAM) is a xanthene dye, a parent of fluorescein dye series. This is a hydrazide derivative for the labeling of carbonyl compounds, aldehydes, and ketones.

Fluorescein is a bright dye that is compatible with a great number of instruments.

Carbohydrates are also often labeled with hydrazides by a protocol involving their oxidation with periodate first, and reacting with dye hydrazide after it.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

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General properties

Appearance: yellow/orange solid
Mass spec M+ increment: 371.1
Molecular weight: 426.81
CAS number: 2183440-65-3 (hydrochloride); 151890-73-2
Molecular formula: C21H15N2ClO6
IUPAC name: Spiro[isobenzofuran-​1(3H)​,​9'-​[9H]​xanthene]​-​6-​carboxylic acid, 3',​6'-​dihydroxy-​3-​oxo-​, hydrazide, hydrochloride
Solubility: good in DMF, DMSO, alcohols
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 522
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17
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