Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 5 days
61020 100 mg $1190.00 in stock

Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.

Absorption and emission spectra of Cyanine3 fluorophore

Absorption and emission spectra of Cyanine3 fluorophore

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Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.

General properties

Appearance: red powder
Mass spec M+ increment: 474.2
Molecular weight: 641.5
CAS number: 1393427-85-4 (without anion), 1393363-07-9 (chloride)
Molecular formula: C34H40N3BF4O4
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​, tetrafluoroborate
Solubility: poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane)
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Hofmann, F.J.; Bodnarchuk, M.; Dirin, D.N.; Vogelsang, J.; Kovalenko, M.V.; Lupton, J.M. Energy transfer from perovskite nanocrystals to dye molecules does not occur by FRET. Nano Letters, 2019, 19(12), 8896–8902. doi: 10.1021/acs.nanolett.9b03779
  2. Rho, J.Y.; Cox, H.; Mansfield, E.D.H.; Ellacott, S.H.; Peltier, R.; Brendel, J.C.; Hartlieb, M.; Waigh, T.A.; Perrier, S. Dual self-assembly of supramolecular peptide nanotubes to provide stabilisation in water. Nature Communications, 2019, 10, 4708. doi: 10.1038/s41467-019-12586-8
  3. Li, L.; Wang, J.; Li, Y.; Radford, D.C.; Yang, J.; Kopeček, J. Broadening and Enhancing Functions of Antibodies by Self-Assembling Multimerization at Cell Surface. ACS Nano, 2019, 13(10), 11422–11432. doi: 10.1021/acsnano.9b04868
  4. Antonov, S.A.; Novosadova, E.V.; Kobylansky, A.G.; Tarantul, V.Z.; Grivennikov, I.A. A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining. Journal of Histochemistry & Cytochemistry, 2019, 67(10), 771–782. doi: 10.1369/0022155419864113
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