Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11020 1 mg $125.00 in stock
21020 5 mg $260.00 in stock
41020 25 mg $510.00 in stock
51020 50 mg $895.00 in stock
61020 100 mg $1490.00 in stock

Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3® and DyLight 549.

Absorption and emission spectra of Cyanine3

Absorption and emission spectra of Cyanine3

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General properties

Appearance: red powder
Mass spec M+ increment: 439.5
Molecular weight: 641.5
CAS number: 2632339-91-2
Molecular formula: C34H40N3BF4O4
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​, tetrafluoroborate
Solubility: poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane)
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Zashikhina, N.; Gladnev, S.; Sharoyko, V.; Korzhikov-Vlakh, V.; Korzhikova-Vlakh, E.; Tennikova, T. Synthesis and Characterization of Nanoparticle-Based Dexamethasone-Polypeptide Conjugates as Potential Intravitreal Delivery Systems. International Journal of Molecular Sciences, 2023, 24(4), 3702. doi: 10.3390/ijms24043702
  2. Meiers, J.; Dastbaz, J.; Adam, S.; Rasheed, S.; Kirsch, S. H.; Meiser, P.; Gross, P.; Müller, R.; Titz, A. Pineapple Lectin AcmJRL Binds SARS-CoV-2 Spike Protein in a Carbohydrate-Dependent Fashion**. ChemBioChem, 2023, 24(3), e202200463. doi: 10.1002/cbic.202200463
  3. Khramtsov, Y. V.; Ulasov, A. V.; Rosenkranz, A. A.; Slastnikova, T. A.; Lupanova, T. N.; Georgiev, G. P.; Sobolev, A. S. An Approach to Evaluate the Effective Cytoplasmic Concentration of Bioactive Agents Interacting with a Selected Intracellular Target Protein. Pharmaceutics, 2023, 15(2), v. doi: 10.3390/pharmaceutics15020324
  4. Yi, G.; Sung, Y.; Kim, C.; Ra, J. S.; Hirakawa, H.; Kato, T. A.; Fujimori, A.; Kim, H.; Takata, K. DNA Polymerase θ-Mediated Repair of High LET Radiation-Induced Complex DNA Double-Strand Breaks. Nucleic Acids Research, 2023, 51(5), 2257–2269. doi: 10.1093/nar/gkad076
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