Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 in stock
61020 100 mg $1190.00 in stock

Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.

Absorption and emission spectra of Cyanine3 fluorophore

Absorption and emission spectra of Cyanine3 fluorophore

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General properties

Appearance: red powder
Mass spec M+ increment: 474.2
Molecular weight: 641.5
CAS number: 1393427-85-4 (without anion), 1393363-07-9 (chloride)
Molecular formula: C34H40N3BF4O4
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​, tetrafluoroborate
Solubility: poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane)
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Chiou, A.E.; Hinckley, J.A.; Khaitan, R:; Varsano, N.; Wang, J.; Malarkey, H.F.; Hernandez, C.J.; Williams, R.M.; Estroff, L.A.; Weiner, S.; Addadi, L.; Wiesner, U.B.; Fischbach, C. Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments. Small, in press. doi: 10.1002/smll.202001432
  2. Aronsson, C.; Jury, M.; Naeimipour, S.; Rasti Boroojeni, F.; Christoffersson, J.; Lifwergren, P.; Mandenius, C.-F.; Selegård, R.; Aili, D. Dynamic peptide-folding mediated biofunctionalization and modulation of hydrogels for 4D bioprinting. Biofabrication, 2020, 12(3), 035031. doi: 10.1088/1758-5090/ab9490
  3. Priwitaningrum, D.L.; Jentsch, J.; Bansal, R.; Rahimian, S.; Storm, G.; Hennink, W.E.; Prakash, J. Apoptosis-inducing Peptide Loaded in PLGA Nanoparticles Induces Anti-tumor Effects in vivo. International Journal of Pharmaceutics, 2020, 585, 119535. doi: 10.1016/j.ijpharm.2020.119535
  4. Deng, L.; Huang, X.; Ren, J. In Situ Study of the Drug-target Protein Interaction in Single Living Cells by Combining Fluorescence Correlation Spectroscopy with Affinity Probes. Analytical Chemistry, 2020, 92(10), 7020–7027. doi: 10.1021/acs.analchem.0c00263
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