Cyanine3 NHS ester

Cyanine3 NHS esters Microscopy
Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 in stock
61020 100 mg $1190.00 in stock
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Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.

Cyanine3 NHS ester
Cyanine3 NHS ester
Cyanine3 NHS ester
Structure of Cyanine3 NHS ester
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Absorption and emission spectra of Cyanine3 fluorophore

Absorption and emission spectra of Cyanine3 fluorophore

Recommended protocol

NHS ester labeling of amino biomolecules

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General properties

Appearance: red powder
Mass spec M+ increment: 474.2
Molecular weight: 641.5
CAS number: 1032678-38-8, 1032815-92-1
Molecular formula: C34H40N3BF4O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

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