Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 in stock
61020 100 mg $1190.00 in stock

Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.

Absorption and emission spectra of Cyanine3 fluorophore

Absorption and emission spectra of Cyanine3 fluorophore

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General properties

Appearance: red powder
Mass spec M+ increment: 474.2
Molecular weight: 641.5
CAS number: 1393427-85-4 (without anion), 1393363-07-9 (chloride)
Molecular formula: C34H40N3BF4O4
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​, tetrafluoroborate
Solubility: poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane)
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Ryu, J.S.; Im, S.H.; Kang, Y.K.; Kim, Y.S.; Chung, H.J. Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives. Biomaterials Science, in press. doi: 10.1039/c9bm01926j
  2. Basu, U.; Lee, S.-W.; Deshpande, A.; Shen, J.; Sohn, B.-K.; Cho, H.; Kim, H.; Patel, S.S. The C-terminal tail of the yeast mitochondrial transcription factor Mtf1 coordinates template strand alignment, DNA scrunching and timely transition into elongation. Nucleic Acids Research, 2020, 48(5), 2604–2620. doi: 10.1093/nar/gkaa040
  3. Hofmann, F.J.; Bodnarchuk, M.; Dirin, D.N.; Vogelsang, J.; Kovalenko, M.V.; Lupton, J.M. Energy transfer from perovskite nanocrystals to dye molecules does not occur by FRET. Nano Letters, 2019, 19(12), 8896–8902. doi: 10.1021/acs.nanolett.9b03779
  4. Rho, J.Y.; Cox, H.; Mansfield, E.D.H.; Ellacott, S.H.; Peltier, R.; Brendel, J.C.; Hartlieb, M.; Waigh, T.A.; Perrier, S. Dual self-assembly of supramolecular peptide nanotubes to provide stabilisation in water. Nature Communications, 2019, 10, 4708. doi: 10.1038/s41467-019-12586-8
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