Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11020 1 mg $91 in stock
21020 5 mg $119 in stock
41020 25 mg $380 in stock
51020 50 mg $670 in stock
61020 100 mg $1150 in stock
81020 1 g please inquire in stock
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Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3® and DyLight 549.

Absorption and emission spectra of Cyanine3

Absorption and emission spectra of Cyanine3

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General properties

Appearance: red powder
Mass spec M+ increment: 439.5
Molecular weight: 641.5
CAS number: 2632339-91-2
Molecular formula: C34H40N3BF4O4
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​, tetrafluoroborate
Solubility: poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane)
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Oh, H. J.; Lee, Y.; Hwang, H.; Hong, K.; Choi, H.; Kang, J. Y.; Jung, Y. Size-Controlled Assembly of Phase Separated Protein Condensates with Interfacial Protein Cages. Nat Commun, 2025, 16(1), 1009. doi: 10.1038/s41467-025-56391-y
  2. Deng, L.; Olea, A.R.; Ortiz‐Perez, A.; Sun, B.; Wang, J.; Pujals, S.; Palmans, A.R.A.; Albertazzi, L. Imaging Diffusion and Stability of Single‐Chain Polymeric Nanoparticles in a Multi‐Gel Tumor‐on‐a‐Chip Microfluidic Device. Small methods, 2024, 2301072. doi: 10.1002/smtd.202301072
  3. Epshtein, M.; Gounis, M.J.; Bogdanov, A.A. X-ray Attenuating Vesicles with Neutrophil Extracellular Trap (NET) Specificity: Synthesis and Testing in a Model System. ACS Omega, 2024, 9(27), 29391-29400. doi: 10.1021/acsomega.4c01525
  4. Xing, W.; Li, D.; Wang, W.; Liu, J.-J.G.; Chen, C. Conformational dynamics of CasX (Cas12e) in mediating DNA cleavage revealed by single-molecule FRET. Nucleic Acids Research, 2024. doi: 10.1093/nar/gkae604
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