Cyanine3 NHS ester

Cyanine3 NHS esters Microscopy Protein labeling
Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 in stock
61020 100 mg $1190.00 in stock
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Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.

Cyanine3 NHS ester
Cyanine3 NHS ester
Cyanine3 NHS ester
Structure of Cyanine3 NHS ester
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Absorption and emission spectra of Cyanine3 fluorophore

Absorption and emission spectra of Cyanine3 fluorophore

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General properties

Appearance: red powder
Mass spec M+ increment: 474.2
Molecular weight: 641.5
CAS number: 1393427-85-4 (without anion), 1393363-07-9 (chloride)
Molecular formula: C34H40N3BF4O4
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​, tetrafluoroborate
Solubility: poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane)
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Chiou, A.E.; Hinckley, J.A.; Khaitan, R:; Varsano, N.; Wang, J.; Malarkey, H.F.; Hernandez, C.J.; Williams, R.M.; Estroff, L.A.; Weiner, S.; Addadi, L.; Wiesner, U.B.; Fischbach, C. Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments. Small, in press. doi: 10.1002/smll.202001432
  2. Li, H.-Y.; Lin, H.-C.; Huang, B.-J.; Lo, A.Z.K.; Saidin, S.; Lai, C.-H. Size Preferences Uptake of Glyco-Silica Nanoparticles to MDA-MB-231 Cell. Langmuir, 2020, 36(38), 11374–11382. doi: 10.1021/acs.langmuir.0c02297
  3. Perkins, L.A.; Nyiranshuti, L.; Little-Ihrig, L.; Latoche, J.D.; Day, K.E.; Zhu, Q.; Tavakoli, S.; Sundd, P.; Novelli, E.M.; Anderson, C.J. Integrin VLA-4 as a PET imaging biomarker of hyper-adhesion in transgenic sickle mice. Blood Advances, 2020, 4(17), 4102–4112. doi: 10.1182/bloodadvances.2020002642
  4. Haddad, Y.; Charousova, M.; Zivotska, H.; Splichal, Z.; Merlos Rodrigo, M.A.; Michalkova, H.; Krizkova, S.; Tesarova, B.; Richtera, L.; Vitek, P.; Stokowa-Soltys, K.; Hynek, D.; Milosavljevic, V.; Rex, S.; Heger, Z. Norepinephrine transporter-derived homing peptides enable rapid endocytosis of drug delivery nanovehicles into neuroblastoma cells. Journal of Nanobiotechnology, 2020, 18, 95. doi: 10.1186/s12951-020-00654-x
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