Cyanine3 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11020 | 1 mg | $110.00 | in stock | |
| 21020 | 5 mg | $210.00 | in stock | |
| 41020 | 25 mg |
$410.00
|
in stock | |
| 51020 | 50 mg |
$695.00
|
in stock | |
| 61020 | 100 mg |
$1190.00
|
in stock |
Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.
Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.
Absorption and emission spectra of Cyanine3 fluorophore
Recommended protocol
Calculator
Customers also purchased with this product
DMF (dimethylformamide), labeling grade
Dry high purity amine free solvent for NHS ester labeling.Cyanine3 amine
Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.ROX azide, 5- isomer
ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.
Add this product to your cart andget free express delivery
General properties
| Appearance: | red powder |
| Mass spec M+ increment: | 474.2 |
| Molecular weight: | 641.5 |
| CAS number: | 1393427-85-4 (without anion), 1393363-07-9 (chloride) |
| Molecular formula: | C34H40N3BF4O4 |
| IUPAC name: | 3H-Indolium, 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-, tetrafluoroborate |
| Solubility: | poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane) |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Chiou, A.E.; Hinckley, J.A.; Khaitan, R:; Varsano, N.; Wang, J.; Malarkey, H.F.; Hernandez, C.J.; Williams, R.M.; Estroff, L.A.; Weiner, S.; Addadi, L.; Wiesner, U.B.; Fischbach, C. Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments. Small, in press. doi: 10.1002/smll.202001432
- Aronsson, C.; Jury, M.; Naeimipour, S.; Rasti Boroojeni, F.; Christoffersson, J.; Lifwergren, P.; Mandenius, C.-F.; Selegård, R.; Aili, D. Dynamic peptide-folding mediated biofunctionalization and modulation of hydrogels for 4D bioprinting. Biofabrication, 2020, 12(3), 035031. doi: 10.1088/1758-5090/ab9490
- Priwitaningrum, D.L.; Jentsch, J.; Bansal, R.; Rahimian, S.; Storm, G.; Hennink, W.E.; Prakash, J. Apoptosis-inducing Peptide Loaded in PLGA Nanoparticles Induces Anti-tumor Effects in vivo. International Journal of Pharmaceutics, 2020, 585, 119535. doi: 10.1016/j.ijpharm.2020.119535
- Deng, L.; Huang, X.; Ren, J. In Situ Study of the Drug-target Protein Interaction in Single Living Cells by Combining Fluorescence Correlation Spectroscopy with Affinity Probes. Analytical Chemistry, 2020, 92(10), 7020–7027. doi: 10.1021/acs.analchem.0c00263
Cy® is a trademark of GE Healthcare.
The count of items is incorrect.
Your cart is empty
$ 
