Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 in stock
61020 100 mg $1190.00 5 days
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Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.

Absorption and emission spectra of Cyanine3 fluorophore

Absorption and emission spectra of Cyanine3 fluorophore

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General properties

Appearance: red powder
Mass spec M+ increment: 474.2
Molecular weight: 641.5
CAS number: 1032678-38-8, 1032815-92-1
Molecular formula: C34H40N3BF4O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Wu, B.; Zhang, H.; Sun, R.; Peng, S.; Cooperman, B.S.; Goldman, Y.E.; Chen, C. Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots. Nucleic Acids Research, in press. doi: 10.1093/nar/gky636
  2. Ye, K.; Sinawang, P.D.; Yoong, A.T.I.; Marks, R.S. Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. Sensors and Actuators B: Chemical, 2018, 256, 234–242. doi: 10.1016/j.snb.2017.10.058
  3. Salinas, C.B.G.; Lu, T.T.-H.; Gabery, S.; Marstal, K.; Alanentalo, T.; Mercer, A.J.; Cornea, A.; Conradsen, K.; Hecksher-Sørensen, J.; Dahl, A.B.; Knudsen, L.B.; Secher, A. Integrated Brain Atlas for Unbiased Mapping of Nervous System Effects Following Liraglutide Treatment. Scientific Reports, 2018, 8, 10310. doi: 10.1038/s41598-018-28496-6
  4. Rho, J.Y.; Brendel, J.C.; MacFarlane, L.R.; Mansfield, E.D.H.; Peltier, R.; Rogers, S.; Hartlieb, M.; Perrier, S. Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells. Advanced Functional Materials, 2018, 28(24), 1704569. doi: 10.1002/adfm.201704569
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