Cyanine3 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11020 | 1 mg | $91 | in stock | |
| 21020 | 5 mg | $119 | in stock | |
| 41020 | 25 mg | $380 | 5 days | |
| 51020 | 50 mg |
$670
|
in stock | |
| 61020 | 100 mg |
$1150
|
in stock | |
| 81020 | 1 g | please inquire | in stock |
Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.
Cyanine3 NHS ester is a replacement for NHS esters of Cy3® and DyLight 549.
Absorption and emission spectra of Cyanine3
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General properties
| Appearance: | red powder |
| Mass spec M+ increment: | 439.5 |
| Molecular weight: | 641.5 |
| CAS number: | 2632339-91-2 |
| Molecular formula: | C34H40N3BF4O4 |
| IUPAC name: | 3H-Indolium, 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-, tetrafluoroborate |
| Solubility: | poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane) |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Oh, H.J.; Lee, Y.; Hwang, H.; Hong, K.; Choi, H.; Kang, J.Y.; Jung, Y. Size-controlled synthesis of phase separated protein condensates with interfacial protein cages. chemRxiv, 2024, preprint. doi: 10.26434/chemrxiv-2024-nkftl
- Deng, L.; Olea, A.R.; Ortiz‐Perez, A.; Sun, B.; Wang, J.; Pujals, S.; Palmans, A.R.A.; Albertazzi, L. Imaging Diffusion and Stability of Single‐Chain Polymeric Nanoparticles in a Multi‐Gel Tumor‐on‐a‐Chip Microfluidic Device. Small methods, 2024, 2301072. doi: 10.1002/smtd.202301072
- Feld, L. G.; Boehme, S. C.; Morad, V.; Sahin, Y.; Kaul, C. J.; Dirin, D. N.; Rainò, G.; Kovalenko, M. V. Quantifying Förster Resonance Energy Transfer from Single Perovskite Quantum Dots to Organic Dyes. ACS Nano, 2024, 18(14), 9997–10007. doi: 10.1021/acsnano.3c11359
- Maltseva, D.; Chatterjee, S.; Yu, C.-C.; Brzezinski, M.; Nagata, Y.; Gonella, G.; Murthy, A. C.; Stachowiak, J. C.; Fawzi, N. L.; Parekh, S. H.; Bonn, M. Fibril Formation and Ordering of Disordered FUS LC Driven by Hydrophobic Interactions. Nature Chemistry, 2023, 15(8), 1146–1154. doi: 10.1038/s41557-023-01221-1
Cy® is a trademark of Cytiva.
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