Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 in stock
61020 100 mg $1190.00 5 days

Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.

Absorption and emission spectra of Cyanine3 fluorophore

Absorption and emission spectra of Cyanine3 fluorophore

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Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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General properties

Appearance: red powder
Mass spec M+ increment: 474.2
Molecular weight: 641.5
CAS number: 1032678-38-8, 1032815-92-1
Molecular formula: C34H40N3BF4O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Zhang, L.; Fang, Y.; Li, L.; Yang, J.; Radford, D.C.; Kopeček, J. Human Serum Albumin-Based Drug-Free Macromolecular Therapeutics: Apoptosis Induction by Coiled-Coil-Mediated Cross-Linking of CD20 Antigens on Lymphoma B Cell Surface. Macromolecular Bioscience, in press. doi: 10.1002/mabi.201800224
  2. Ye, K.; Sinawang, P.D.; Yoong, A.T.I.; Marks, R.S. Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. Sensors and Actuators B: Chemical, 2018, 256, 234–242. doi: 10.1016/j.snb.2017.10.058
  3. Swaminathan, J.; Boulgakov, A.A.; Hernandez, E.T.; Bardo, A.M.; Bachman, J.L.; Marotta, J.; Johnson, A.M.; Anslyn, E.V.; Marcotte, E.M. Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures. Nature Biotechnology, 2018, 36(11), 1076–1082. doi: 10.1038/nbt.4278
  4. Smith, J.D.; Cardwell, L.N.; Porciani, D.; Nguyen, J.A.; Gallazzi, F.; Tata, R.R.; Burke, D.H.; Daniels, M.A.; Ulery, B. Aptamer-displaying peptide amphiphile micelles as a cell-targeted delivery vehicle of peptide cargoes. Physical Biology, 2018, 15(6), 065006. doi: 10.1088/1478-3975/aadb68
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