Cyanine3 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11020 | 1 mg | $110.00 | in stock | |
| 21020 | 5 mg | $210.00 | in stock | |
| 41020 | 25 mg |
$410.00
|
in stock | |
| 51020 | 50 mg |
$695.00
|
in stock | |
| 61020 | 100 mg |
$1190.00
|
in stock |
Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.
Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.
Absorption and emission spectra of Cyanine3 fluorophore
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Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.Amino-11-dUTP
Amino-11-dUTP is a triphosphate for the modification of DNA.General properties
| Appearance: | red powder |
| Mass spec M+ increment: | 474.2 |
| Molecular weight: | 641.5 |
| CAS number: | 1032678-38-8, 1032815-92-1 |
| Molecular formula: | C34H40N3BF4O4 |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
Spectral properties
| Excitation maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Zhang, L.; Fang, Y.; Li, L.; Yang, J.; Radford, D.C.; Kopeček, J. Human Serum Albumin-Based Drug-Free Macromolecular Therapeutics: Apoptosis Induction by Coiled-Coil-Mediated Cross-Linking of CD20 Antigens on Lymphoma B Cell Surface. Macromolecular Bioscience, in press. doi: 10.1002/mabi.201800224
- Ye, K.; Sinawang, P.D.; Yoong, A.T.I.; Marks, R.S. Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. Sensors and Actuators B: Chemical, 2018, 256, 234–242. doi: 10.1016/j.snb.2017.10.058
- Swaminathan, J.; Boulgakov, A.A.; Hernandez, E.T.; Bardo, A.M.; Bachman, J.L.; Marotta, J.; Johnson, A.M.; Anslyn, E.V.; Marcotte, E.M. Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures. Nature Biotechnology, 2018, 36(11), 1076–1082. doi: 10.1038/nbt.4278
- Smith, J.D.; Cardwell, L.N.; Porciani, D.; Nguyen, J.A.; Gallazzi, F.; Tata, R.R.; Burke, D.H.; Daniels, M.A.; Ulery, B. Aptamer-displaying peptide amphiphile micelles as a cell-targeted delivery vehicle of peptide cargoes. Physical Biology, 2018, 15(6), 065006. doi: 10.1088/1478-3975/aadb68
Cy® is a trademark of GE Healthcare.
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