Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11020 1 mg $91 in stock
21020 5 mg $119 in stock
41020 25 mg $380 in stock
51020 50 mg $670 in stock
61020 100 mg $1150 in stock
81020 1 g please inquire in stock
Found better price? Let us know and we will propose the way forward!

Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3® and DyLight 549.

Absorption and emission spectra of Cyanine3

Absorption and emission spectra of Cyanine3

Customers also purchased with this product

Cyanine7 amine

Cy7 amine is a near-infrared dye with free amine group for the conjugation with activated esters and other reactive molecules.
Add this product to your cart and
get free express delivery

Pyrenebutyric acid NHS ester

Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.

Cyanine3 carboxylic acid

The free carboxylic acid of Cyanine3 dye. Non-sulfonated dye, with good solubility in organic solvents, and limited aqueous solubility.

General properties

Appearance: red powder
Mass spec M+ increment: 439.5
Molecular weight: 641.5
CAS number: 2632339-91-2
Molecular formula: C34H40N3BF4O4
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​, tetrafluoroborate
Solubility: poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane)
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Oh, H. J.; Lee, Y.; Hwang, H.; Hong, K.; Choi, H.; Kang, J. Y.; Jung, Y. Size-Controlled Assembly of Phase Separated Protein Condensates with Interfacial Protein Cages. Nat Commun, 2025, 16(1), 1009. doi: 10.1038/s41467-025-56391-y
  2. Deng, L.; Olea, A.R.; Ortiz‐Perez, A.; Sun, B.; Wang, J.; Pujals, S.; Palmans, A.R.A.; Albertazzi, L. Imaging Diffusion and Stability of Single‐Chain Polymeric Nanoparticles in a Multi‐Gel Tumor‐on‐a‐Chip Microfluidic Device. Small methods, 2024, 2301072. doi: 10.1002/smtd.202301072
  3. Epshtein, M.; Gounis, M.J.; Bogdanov, A.A. X-ray Attenuating Vesicles with Neutrophil Extracellular Trap (NET) Specificity: Synthesis and Testing in a Model System. ACS Omega, 2024, 9(27), 29391-29400. doi: 10.1021/acsomega.4c01525
  4. Xing, W.; Li, D.; Wang, W.; Liu, J.-J.G.; Chen, C. Conformational dynamics of CasX (Cas12e) in mediating DNA cleavage revealed by single-molecule FRET. Nucleic Acids Research, 2024. doi: 10.1093/nar/gkae604
Show more (142)
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.