Cyanine5.5 NHS ester

Cyanine5.5 NHS esters Microscopy Protein labeling
Cat. # Quantity Price Lead time
17020 1 mg $110.00 in stock
27020 5 mg $210.00 in stock
47020 25 mg $410.00 in stock
57020 50 mg $695.00 in stock
67020 100 mg $1190.00 in stock
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Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.

Cyanine5.5 NHS ester
Cyanine5.5 NHS ester
Cy5.5 NHS ester structure
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Cy5.5 absorbance and emission spectra

Cy5.5 absorbance and emission spectra

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3-Ethynyl perylene

Alkyne derivative of ultra-bright and photostable perylene fluorophore.

General properties

Appearance: dark blue solid
Molecular weight: 767.66
CAS number: 2375105-86-3
Molecular formula: C44H46N3BF4O4
IUPAC name: 1H-​Benz[e]​indolium, 2-​[5-​(1,​3-​dihydro-​1,​1,​3-​trimethyl-​2H-​benz[e]​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​3-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​1,​1-​dimethyl-
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Product citations

  1. Nam, S.H.; Jang, J.; Cheon, D.H.; Chong, S.-E.; Ahn, J.H.; Hyun, S.; Yu, J.; Lee, Y. pH-Activatable cell penetrating peptide dimers for potent delivery of anticancer drug to triple-negative breast cancer. Journal of Controlled Release, 2021, 330, 898–906. doi: 10.1016/j.jconrel.2020.10.063
  2. Wang, Y.; Xu, X.; Zhao, X.; Yin, Z. Functionalized polymeric hybrid micelles as an efficient nanotheranostic agent for thrombus imaging and thrombolysis. Acta Biomaterialia, 2021, 122, 278–290. doi: 10.1016/j.actbio.2020.10.015
  3. Nguyen, H.T.; Phung, C.D.; Tran, T.H.; Pham, T.T.; Pham, L.M.; Nguyen, T.T.; Jeong, J.-H.; Choi, H.-G.; Ku, S.K.; Yong, C.S.; Kim, J.O. Manipulating immune system using nanoparticles for an effective cancer treatment: Combination of targeted therapy and checkpoint blockage miRNA. Journal of Controlled Release, 2021, 329, 524–537. doi: 10.1016/j.jconrel.2020.09.034
  4. Ji, M.-L.; Jiang, H.; Wu, F.; Geng, R.; Ya, L.K.; Lin, Y.C.; Xu, J.H.; Wu, X.T.; Lu, J. Precise targeting of miR-141/200c cluster in chondrocytes attenuates osteoarthritis development. Annals of the Rheumatic Diseases, 2021, 80(3), 356–366. doi: 10.1136/annrheumdis-2020-218469
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Cy® is a trademark of GE Healthcare.
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