Cyanine5.5 NHS ester

Cyanine5.5 NHS esters Microscopy Protein labeling
Cat. # Quantity Price Lead time
17020 1 mg $110.00 in stock
27020 5 mg $210.00 in stock
47020 25 mg $410.00 in stock
57020 50 mg $695.00 in stock
67020 100 mg $1190.00 in stock
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Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.

Cyanine5.5 NHS ester
Cyanine5.5 NHS ester
Cy5.5 NHS ester structure
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Cy5.5 absorbance and emission spectra

Cy5.5 absorbance and emission spectra

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General properties

Appearance: dark blue solid
Molecular weight: 767.66
CAS number: 1469277-96-0, 2054318-82-8 (chloride); 1469541-53-4, 1874250-60-8 (without anion)
Molecular formula: C44H46N3BF4O4
IUPAC name: 1H-​Benz[e]​indolium, 2-​[5-​(1,​3-​dihydro-​1,​1,​3-​trimethyl-​2H-​benz[e]​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​3-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​1,​1-​dimethyl-
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 209000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Product citations

  1. Kashfi-Sadabad, R.; Gonzalez-Fajardo, L.; Hargrove, D.; Ahmadi, B:, Munteanu, D.; Shahbazmohamadi, S.; Jay, M.; Lu, X. Engineering Multifunctional Gold Decorated Dendritic Mesoporous Silica/Tantalum Oxide Nanoparticles for Intraperitoneal Tumor-Specific Delivery. Particle & Particle Systems Characterization, in press. doi: 10.1002/ppsc.201900058
  2. Guo, J.; Mingoes, C.; Qiu, X.; Hildebrandt, N. Simple, Amplified, and Multiplexed Detection of MicroRNAs Using Time-Gated FRET and Hybridization Chain Reaction. Analytical Chemistry, 2019, 91(4), 3101–3109. doi: 10.1021/acs.analchem.8b05600
  3. Shim, G.; Le, Q.-V.; Suh, J.; Choi, S.; Kim, G.; Choi, H.-G.; Kim, Y.B.; Macgregor, M.B.; Oh, Y.-K. Sequential activation of anticancer therapy triggered by tumor microenvironment-selective imaging. Journal of Controlled Release, 2019, 298, 110–119. doi: 10.1016/j.jconrel.2019.02.012
  4. Chen, Y.; Fan, H.; Xu, C.; Hu, W.; Yu, B. Efficient Cholera Toxin B Subunit-Based Nanoparticles with MRI Capability for Drug Delivery to the Brain Following Intranasal Administration. Macromolecular Bioscience, 2019, 19(2), 1800340. doi: 10.1002/mabi.201800340
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Cy® is a trademark of GE Healthcare.
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