Cyanine5.5 NHS ester

Cat. # Quantity Price Lead time
17020 1 mg $125 in stock
27020 5 mg $260 in stock
47020 25 mg $510 in stock
57020 50 mg $895 in stock
67020 100 mg $1490 in stock
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Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5® and DyLight 680.

Cy5.5 absorbance and emission spectra

Cy5.5 absorbance and emission spectra

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General properties

Appearance: dark blue to violet solid
Molecular weight: 767.66
CAS number: 2375105-86-3
Molecular formula: C44H46N3BF4O4
IUPAC name: 1H-​Benz[e]​indolium, 2-​[5-​(1,​3-​dihydro-​1,​1,​3-​trimethyl-​2H-​benz[e]​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​3-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​1,​1-​dimethyl-
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Product citations

  1. Yun, W.S.; Yang, W.; Shim, M.K.; Song, S.; Choi, J.; Kim, J.; Kim, J.; Moon, Y.; Jo, S.H.; Lim, D.-K.; Kim, K. Accurately controlled tumor temperature with silica-coated gold nanorods for optimal immune checkpoint blockade therapy. Biomaterials Research, 2024, 28, 0024. doi: 10.34133/bmr.0024
  2. Liu, Q.; Wang, C.; Zhu, M.; Liu, J.; Duan, Q.; Midgley, A.C.; Liu, R.; Jiang, B.; Kong, D.; Chen, Q.; Zhuang, J.; Huang, X. Self‐Assembly of Heterogeneous Ferritin Nanocages for Tumor Uptake and Penetration. Advanced Science, 2024, 11(17), 2309271. doi: 10.1002/advs.202309271
  3. Han, G.; Kim, H.; Jang, H.; Kim, E.S.; Kim, S.H.; Yang, Y. Oral TNF-α siRNA delivery via milk-derived exosomes for effective treatment of inflammatory bowel disease. Bioactive Materials, 2024, 34, 138-149. doi: 10.1016/j.bioactmat.2023.12.010
  4. Halim, A.; Al-Qadi, N.; Kenyon, E.; Conner, K.N.; Mondal, S.K.; Medarova, Z.; Moore, A. Inhibition of miR-10b treats metastatic breast cancer by targeting stem cell-like properties. Oncotarget, 2024, 15, 591-606. doi: 10.18632/oncotarget.28641
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