Cyanine5.5 NHS ester

Cat. # Quantity Price Lead time
17020 1 mg $110.00 in stock
27020 5 mg $210.00 in stock
47020 25 mg $410.00 in stock
57020 50 mg $695.00 in stock
67020 100 mg $1190.00 in stock

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.

Cy5.5 absorbance and emission spectra

Cy5.5 absorbance and emission spectra

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General properties

Appearance: dark blue solid
Molecular weight: 767.66
CAS number: 1469277-96-0 [chloride]
Molecular formula: C44H46N3BF4O4
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 209000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Product citations

  1. Bellat, V.; Ting, R.; Southard, T.L.; Vahdat, L.; Molina, H.; Fernandez, J.; Aras, O.; Stokol, T.; Law, B. Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme-Induced Local Retention Properties. Advanced Functional Materials, 2018, 28(44), 1803969. doi: 10.1002/adfm.201803969
  2. Moon, C.H.; Lee, J.Y.; Kim, E.S.; Park, J.H.; Kim, S.-Y.; Kim, J.Y.; Tchah, H. In vivo biodistribution of topical low molecular weight heparin-taurocholate in a neovascularized mouse cornea. International Journal of Ophthalmology, 2018, 11(9), 1435–1439. doi: 10.18240/ijo.2018.09.01
  3. Lv, J.; He, B.; Yu, J.; Wang, Y.; Wang, C.; Zhang, S.; Wang, H.; Hu, J.; Zhang, Q.; Cheng, Y. Fluoropolymers for intracellular and in vivo protein delivery. Biomaterials, 2018, 182, 167–175. doi: 10.1016/j.biomaterials.2018.08.023
  4. Machová, D.; Koziolová, E.; Chytil, P.; Venclíková, K.; Etrych, T.; Janoušková, O. Nanotherapeutics with suitable properties for advanced anticancer therapy based on HPMA copolymer-bound ritonavir via pH-sensitive spacers. European Journal of Pharmaceutics and Biopharmaceutics, 2018, 131, 141–150. doi: 10.1016/j.ejpb.2018.07.023
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