Biotin phosphoramidite (hydroxyprolinol)
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22360
250 mg
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Non-nucleosidic modifier reagent for the synthesis of biotinylated oligos based on a hydroxyprolinol core. This phosphoramidite contains a dimethoxytrityl protective group for the purification of the resulting oligos on C18 cartridges, and reverse phase HPLC. This modifier reagent can be used to introduce biotin onto 5'-position, 3'-position (using universal CPG), and internal oligo sites.
This amidite does not require special condensation and deprotection conditions.
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General properties
Appearance:
white powder
Molecular weight:
959.18
Molecular formula:
C51 H71 N6 O8 PS
Solubility:
good in MeCN, DCM, THF
Quality control:
NMR 1 H, 31 P, HPLC-MS (95%), functional testing in oligo synthesis
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:
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Product specifications
Oligo synthesis details
Diluent:
acetonitrile
Coupling conditions:
standard coupling, identical to normal nucleobases
Deprotection conditions:
identical to protected nucleobases
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