Phosphate CPG 500 solid support

Cat. # Quantity Price Lead time
13860 100 mg $40 in stock
43860 1 g $170 in stock
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Phosphate CPG is a solid support for the synthesis of 3'-phosphorylated oligonucleotides. The phosphate group can be used as a blocking functionality to prevent 3'-5' exonuclease activity of some polymerases.

Upon cleavage from the support, a phosphate group remains at the 3' end of the oligonucleotide. It does not require any changes in oligo synthesis and deprotection protocols.

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General properties

Appearance: off-white beads
Quality control: NMR 1H, HPLC-MS (95%) of bound reagent, loading measurement, functional testing (oligo synthesis)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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