ROX NHS ester, 6- isomer

Cat. # Quantity Price Lead time
12220 1 mg $110.00 in stock
22220 5 mg $210.00 5 days
42220 25 mg $410.00 in stock
52220 50 mg $695.00 in stock
62220 100 mg $1190.00 in stock

ROX (rhodamine X aka R101) is a highly fluorescent, and photostable rhodamine dye for various applications. ROX labeled oligonucleotide probes are often used in qPCR, and qPCR instruments have ROX channel.

This is reactive dye for the labeling of amino-groups in peptides, proteins, and amino-oligonucleotides. Pure single isomer.

Absorption and emission spectra of ROX dye

Absorption and emission spectra of ROX dye

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General properties

Appearance: dark crimson powder
Mass spec M+ increment: 516.2
Molecular weight: 631.67
CAS number: 117491-83-5; 1890922-83-4; 216699-36-4
Molecular formula: C37H33N3O7
Solubility: good in polar organic solvents (DMF, DMSO), low in water
Quality control: NMR 1H, HPLC-MS (80+%, balance mostly free carboxylic acid)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 88000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.0
CF260: 0.62
CF280: 0.49

Product citations

  1. Konopka, C.J.; Wozniak, M.; Hedhli, J.; Ploska, A.; Schwartz-Duval, A.; Siekierzycka, A.; Pan, D.; Munirathinam, G.; Dobrucki, I.T.; Kalinowski, L.; Dobrucki, L.W. Multimodal imaging of the receptor for advanced glycation end-products with molecularly targeted nanoparticles. Theranostics, 2018, 8(18), 5012–5024. doi: 10.7150/thno.24791
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