Cyanine5 phosphoramidite

Cat. # Quantity Price Lead time
1740-100mg 100 mg –   in stock
1740-250mg 250 mg $290.00 10 days
1740-1g 1 g $950.00 in stock
1740-10g 10 g $6750.00 10 days

Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.

This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent.

Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases stability of the reagent, maintaining efficient coupling over longer time.

Absorption and emission spectra of Cyanine5

Absorption and emission spectra of Cyanine5

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General properties

Appearance: dark colored solid
Molecular weight: 816.49
Molecular formula: C47H67N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
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