Cyanine5 phosphoramidite

Cat. # Quantity Price Lead time
1740-100mg 100 mg –   in stock
1740-250mg 250 mg $290 in stock
1740-1g 1 g $950 in stock
1740-10g 10 g $6750 7 days
Found better price? Let us know and we will propose the way forward!

Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.

This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent.

Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases stability of the reagent, maintaining efficient coupling over longer time.

Absorption and emission spectra of Cyanine5

Absorption and emission spectra of Cyanine5

Customers also purchased with this product

R6G phosphoramidite, 6-isomer

Phosphoramidite for synthesis of oligonucleotides labeled at the 5'-terminus with rhodamine 6G (R6G). Pure 6-isomer.
Add this product to your cart and
get free express delivery

DusQ 2 CPG 1000

Controlled pore glass (CPG) 1000 modified support for the synthesis of oligonucleotides with DusQ 2 quencher at the 3’ end. Compatible with standard deblocking conditions.
Add this product to your cart and
get free express delivery

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester for the labeling of amino-groups.

General properties

Appearance: dark colored solid
Molecular weight: 816.49
Molecular formula: C47H67N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.