TAMRA phosphoramidite, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2318-100mg | 100 mg |
$149.90
|
in stock | |
| 2318-250mg | 250 mg |
$429.90
|
in stock | |
| 2318-1g | 1 g |
$919.90
|
in stock | |
| 2318-5g | 5 g |
please inquire
|
in stock |
This phosphoramidite is used for synthesis of oligonucleotides 5′-labeled with TAMRA.
TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5′-terminal TAMRA and FAM in the middle of the sequence or at the 3′-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively).
TAMRA 5′-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently.
The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Absorption and emission spectra of 6-TAMRA
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General properties
| Appearance: | red solid |
| Mass spec M+ increment: | 589.60 |
| Molecular weight: | 727.83 |
| Molecular formula: | C40H50N5O6P |
| Solubility: | good in acetonitrile, DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS (95%), functional testing |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Coupling conditions: | coupling time 7.5 min |
| Deprotection conditions: | Tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature. Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support. |
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