Fluorescein-dT phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13460 | 100 mg | – | 5 days | |
| 23460 | 250 mg |
$410.00
|
in stock | |
| 43460 | 1 g |
$1590.00
|
in stock | |
| 63460 | 5 g |
$6900.00
|
in stock | |
| 83460 | 10 g |
please inquire
|
in stock |
This phosphoramidite allows fluorescein (FAM) to be introduced into any position in the oligonucleotide sequence during synthesis by the phosphoramidite method (in the middle of the chain, at the 5’- and 3’-terminus). The reagent is a conjugate of deoxythymidine phosphoramidite and 6-isomer of FAM. Modification is performed during oligonucleotide synthesis by substituting standard dT phosphoramidite with fluorescein-dT phosphoramidite. This modification does not affect exonuclease or polymerase activity.
For modification with fluorescein at the 5’-terminus use FAM phosphoramidite, 6-isomer.
Usage
Coupling: 10 minutes
Deprotection: standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).
Absorption and emission spectra of FAM
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Phosphoramidite for synthesis of oligonucleotides labeled at the 5'-terminus with rhodamine 6G (R6G). Pure 6-isomer.General properties
| Appearance: | off white powder |
| Molecular weight: | 1423.54 |
| Molecular formula: | C79H87N6O17P |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 490 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 513 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
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