FAM NHS ester, 6-isomer

Cat. # Quantity Price Lead time
15120 1 mg $110.00 in stock
25120 5 mg $160.00 in stock
45120 25 mg $210.00 in stock
55120 50 mg $270.00 in stock
65120 100 mg $420.00 in stock

Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.

Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.

This product contains the pure 6-isomer of fluorescein.

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General properties

Appearance: yellow crystalline solid
Mass spec M+ increment: 358.0
Molecular weight: 473.39
Molecular formula: C25NO9H15
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Product citations

  1. Evans, L.E.; Jones, K.; Cheeseman, M.D. Targeting secondary protein complexes in drug discovery: studying the druggability and chemical biology of the HSP70/BAG1 complex. Chemical Communications, 2017, 53(37), 5167–5170. doi: 10.1039/c7cc01376k
  2. Warminski, M.; Sikorski, P.J.; Warminska, Z.; Lukaszewicz, M.; Kropiwnicka, A.; Zuberek, J.; Darzynkiewicz, E.; Kowalska, J.; Jemielity, J. Amino-functionalized 5' cap analogs as tools for site-specific sequence-independent labeling of messenger RNA. Bioconjugate Chemistry, 2017, 28(7), 1978–1992. doi: 10.1021/acs.bioconjchem.7b00291
  3. Horning, D.P.; Joyce, G.F. Amplification of RNA by an RNA polymerase ribozyme. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(35), 9786–9791. doi: 10.1073/pnas.1610103113
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