FAM NHS ester, 6-isomer

Cat. # Quantity Price Lead time
15120 1 mg –   in stock
25120 5 mg $110 in stock
45120 25 mg $145 in stock
55120 50 mg $260 in stock
65120 100 mg $470 in stock
85120 1 g please inquire in stock
95120 5 g please inquire in stock
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Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.

Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.

This product contains the pure 6-isomer of fluorescein.

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General properties

Appearance: yellow crystalline solid
Mass spec M+ increment: 358.0
Molecular weight: 473.39
CAS number: 92557-81-8
Molecular formula: C25NO9H15
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17

Product citations

  1. Haynes, J.R.; Whitmore, C.A.; Behof, W.J.; Landman, C.A.; Ong, H.H.; Feld, A.P.; Suero, I.C.; Greer, C.B.; Gore, J.C.; Wijesinghe, P.; Matsubara, J.A.; Wadzinski, B.E.; Spiller, B.W.; Pham, W. Targeting Soluble Amyloid-Beta Oligomers With a Novel Nanobody. Research Square, 2024, preprint. doi: 10.21203/rs.3.rs-3944211/v1
  2. Chen, Z.; Zakrzewska, S.; Hajare, H.S.; Du Bois, J.; Minor, D.L. Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry. STAR Protocols, 2024, 5(1), 102792. doi: 10.1016/j.xpro.2023.102792
  3. Kropacheva, N. O.; Golyshkin, A. A.; Vorobyeva, M. A.; Meschaninova, M. I. Convenient Solid-Phase Attachment of Small-Molecule Ligands to Oligonucleotides via a Biodegradable Acid-Labile P-N-Bond. Molecules, 2023, 28(4), 1904. doi: 10.3390/molecules28041904
  4. Liu, X.; Ren, X.; Zhou, L.; Liu, K.; Deng, L.; Qing, Q.; Li, J.; Zhi, F.; Li, M. Tollip Orchestrates Macrophage Polarization to Alleviate Intestinal Mucosal Inflammation. Journal of Crohn’s and Colitis, 2022, 16(7), 1151–1167. doi: 10.1093/ecco-jcc/jjac019
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