FAM NHS ester, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15120 | 1 mg |
–
|
in stock | |
| 25120 | 5 mg |
$110
|
in stock | |
| 45120 | 25 mg |
$145
|
in stock | |
| 55120 | 50 mg |
$260
|
in stock | |
| 65120 | 100 mg |
$470
|
in stock | |
| 85120 | 1 g |
please inquire
|
in stock | |
| 95120 | 5 g |
please inquire
|
in stock |
Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.
Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.
This product contains the pure 6-isomer of fluorescein.
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EdU (5-Ethynyl-2'-deoxyuridine)
EdU is a nucleoside that is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by click chemistry reaction with fluorescent dye azides to reveal cell proliferation.sulfo-Cyanine3.5 NHS ester
Amine-reactive activated ester of water-soluble, sulfonated sulfo-Cyanine3.5 dye.FAM phosphoramidite, 6-isomer
FAM (fluorescein) phosphoramidite 6-isomer, is a widely used reagent in nucleic acid chemistry for labeling oligonucleotides. It is derived from the single pure isomer of 6-carboxyfluorescein and separated from amidite linkage via C6 aminohexanol spacer.General properties
| Appearance: | yellow crystalline solid |
| Mass spec M+ increment: | 358.0 |
| Molecular weight: | 473.39 |
| CAS number: | 92557-81-8 |
| Molecular formula: | C25NO9H15 |
| IUPAC name: | (2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
Product citations
- Chen, Z.; Zakrzewska, S.; Hajare, H.S.; Du Bois, J.; Minor, D.L. Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry. STAR Protocols, 2024, 5(1), 102792. doi: 10.1016/j.xpro.2023.102792
- Kang, N.-W.; Seo, Y.A.; Jackson, K.J.; Jang, K.; Song, E.; Han, U.; Chen, F.; Heilshorn, S.C.; Myung, D. Photoactivated growth factor release from bio-orthogonally crosslinked hydrogels for the regeneration of corneal defects. Bioactive Materials, 2024, 40, 417-429. doi: 10.1016/j.bioactmat.2024.05.045
- Haynes, J.R.; Whitmore, C.A.; Behof, W.J.; Landman, C.A.; Ong, H.H.; Feld, A.P.; Suero, I.C.; Greer, C.B.; Gore, J.C.; Wijesinghe, P.; Matsubara, J.A.; Wadzinski, B.E.; Spiller, B.W.; Pham, W. Targeting soluble amyloid-beta oligomers with a novel nanobody. Scientific Reports, 2024, 14, 16086. doi: 10.1038/s41598-024-66970-6
- Kropacheva, N. O.; Golyshkin, A. A.; Vorobyeva, M. A.; Meschaninova, M. I. Convenient Solid-Phase Attachment of Small-Molecule Ligands to Oligonucleotides via a Biodegradable Acid-Labile P-N-Bond. Molecules, 2023, 28(4), 1904. doi: 10.3390/molecules28041904
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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