TAMRA carboxylic acid, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17190 | 1 mg | – | in stock | |
| 27190 | 5 mg | $110 | in stock | |
| 47190 | 25 mg | $125 | in stock | |
| 57190 | 50 mg | $185 | in stock |
Tetramethylrhodamine (TAMRA) is a xanthene dye with orange emission. The dye is a FRET acceptor for FAM and is sometimes used as a quencher of FAM. Like other xanthenes, TAMRA exists as two isomers (5- and 6-) with similar spectral properties. This compound is a pure 5-isomer of TAMRA.
TAMRA carboxylic acid is a non-reactive form of TAMRA dye that can be used as a reference standard in experiments involving TAMRA dye conjugates. Besides, the carboxylic group can react with hydrazines, hydroxylamines, and amines using carbodiimides such as EDAC.
Absorption and emission spectra of 5-TAMRA
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Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides. Pure 5-isomer.General properties
| Appearance: | green powder |
| Molecular weight: | 430.46 |
| Molecular formula: | C25H22N2O5 |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
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