TAMRA carboxylic acid, 5-isomer

Cat. # Quantity Price Lead time
17190 1 mg –   in stock
27190 5 mg $110 in stock
47190 25 mg $125 in stock
57190 50 mg $185 in stock
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Tetramethylrhodamine (TAMRA) is a xanthene dye with orange emission. The dye is a FRET acceptor for FAM and is sometimes used as a quencher of FAM. Like other xanthenes, TAMRA exists as two isomers (5- and 6-) with similar spectral properties. This compound is a pure 5-isomer of TAMRA.

TAMRA carboxylic acid is a non-reactive form of TAMRA dye that can be used as a reference standard in experiments involving TAMRA dye conjugates. Besides, the carboxylic group can react with hydrazines, hydroxylamines, and amines using carbodiimides such as EDAC.

Absorption and emission spectra of 5-TAMRA

Absorption and emission spectra of 5-TAMRA

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TAMRA NHS ester, 5-isomer

Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides. Pure 5-isomer.

General properties

Appearance: green powder
Molecular weight: 430.46
Molecular formula: C25H22N2O5
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
Fluorescence quantum yield: 0.1
CF260: 0.32
CF280: 0.19
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