Cyanine5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13080 | 1 mg | $110.00 | in stock | |
| 23080 | 5 mg | $210.00 | in stock | |
| 43080 | 25 mg |
$410.00
|
in stock | |
| 53080 | 50 mg |
$695.00
|
in stock | |
| 63080 | 100 mg |
$1190.00
|
in stock |
Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.
Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.
For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.
Cy5 excitation and emission spectra
Recommended protocol
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EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.Cyanine5 NHS ester minimal dye
Cy5 NHS ester minimal dye for protein labeling.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 605.8 |
| CAS number: | 1437872-46-2 (without anion) |
| Molecular formula: | C38H45N4O3 |
| IUPAC name: | 3H-Indolium, 2-[5-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl- |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Pishesha, N.; Harmand, T.; Smeding, L.; Ma, W.; Ludwig, L.; Janssen, R.; Islam, A.; Xie, Y.; Fang, T.; McCaul, N.; Pinney, W.; Sugito, H.; Ploegh, H. Single domain antibody-antigen adducts that target Class II MHC induce antigen-specific tolerance. Research Square, preprint. doi: 10.21203/rs.3.rs-192181/v1
- Delvaux, N.A.; Mathew, B.; Rice, K.G. Fluorescent Labeling of Plasmid DNA for Gene Delivery: Implications of Dye Hydrophobicity on Labeling Efficiencies and Nanoparticle Size. Analytical Biochemistry, in press. doi: 10.1016/j.ab.2020.113895
- Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
- Ast, J.; Arvaniti, A.; Fine, N.H.F.; Nasteska, D.; Ashford, F.B.; Stamataki, Z.M Koszegi, Z.; Bacon, A.; Trapp, S.; Jones, B.J.; Hastoy, B.; Tomas, A.; Reissaus, C.; Linnemann, A.K.; D'Este, E.; Calebiro, D.; Johnsson, K.; Podewin, T.; Broichhagen, J.; Hodson, D.J. LUXendins reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. bioRxiv, preprint. doi: 10.1101/557132
Cy® is a trademark of GE Healthcare.
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