Cyanine5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13080 | 1 mg | $110.00 | in stock | |
| 23080 | 5 mg | $210.00 | in stock | |
| 43080 | 25 mg |
$410.00
|
in stock | |
| 53080 | 50 mg |
$695.00
|
in stock | |
| 63080 | 100 mg |
$1190.00
|
5 days |
Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.
Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.
For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.
Cy5 excitation and emission spectra
Recommended protocol
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Cyanine5 alkyne
Cyanine5 alkyne is an alkyne dye for Click Chemistry.Biotin alkyne
Biotin alkyne is a reactive affinity label which can be attached to biomolecules via Click Chemistry.Sulfo-Cyanine5.5 amine
Sulfo-Cyanine5.5 is a far red/NIR fluorophore possessing high hydrophilicity and aqueous solubility. The dye displays good brightness in far red region due to outstanding molar extinction coefficient. This is amine derivative which is reactive towards electrophiles. It can also be used for enzymatic transamination labeling.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 641.24 |
| CAS number: | 1437872-46-2 (without anion), 1437796-65-0 (chloride) |
| Molecular formula: | C38H45ClN4O3 |
| IUPAC name: | 3H-Indolium, 2-[5-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl- |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
- Ast, J.; Arvaniti, A.; Fine, N.H.F.; Nasteska, D.; Ashford, F.B.; Stamataki, Z.M Koszegi, Z.; Bacon, A.; Trapp, S.; Jones, B.J.; Hastoy, B.; Tomas, A.; Reissaus, C.; Linnemann, A.K.; D'Este, E.; Calebiro, D.; Johnsson, K.; Podewin, T.; Broichhagen, J.; Hodson, D.J. LUXendins reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. bioRxiv, preprint. doi: 10.1101/557132
- Kesarwani, V.; Kelly, H.G.; Shankar, M.; Robinson, K.J.; Kent, S.J.; Traven, A.; Corrie, S.R. Characterisation of key bio-nano interactions between organosilica nanoparticles and Candida albicans. ACS Applied Materials & Interfaces, 2019, 11(38), 34676–34687. doi: 10.1021/acsami.9b10853
- Gardinier, T.C.; Turker, M.Z.; Hinckley, J.A.; Katt, W.P.; DomNwachukwu, N.; Woodruff, F.; Hersh, J.; Wang, J.; Cerione, R.A.; Wiesner, U.B. Controlling Surface Chemical Heterogeneities of Ultrasmall Fluorescent Core–Shell Silica Nanoparticles as Revealed by High-Performance Liquid Chromatography. The Journal of Physical Chemistry C, 2019, 123(37), 23246–23254. doi: 10.1021/acs.jpcc.9b06905
Cy® is a trademark of GE Healthcare.
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