Cyanine5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13080 | 1 mg | $110.00 | in stock | |
| 23080 | 5 mg | $210.00 | in stock | |
| 43080 | 25 mg |
$410.00
|
in stock | |
| 53080 | 50 mg |
$695.00
|
in stock | |
| 63080 | 100 mg |
$1190.00
|
in stock |
Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.
Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.
For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.
Cy5 excitation and emission spectra
Recommended protocol
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Near-infrared fluorescent dye for Click Chemistry.Coumarin 343 X NHS ester
Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.Cyanine3 DBCO
Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper free Click chemistry.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 641.24 |
| CAS number: | 1437872-46-2 (without anion), 1437796-65-0 (chloride) |
| Molecular formula: | C38H45ClN4O3 |
| IUPAC name: | 3H-Indolium, 2-[5-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl- |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Delvaux, N.A.; Mathew, B.; Rice, K.G. Fluorescent Labeling of Plasmid DNA for Gene Delivery: Implications of Dye Hydrophobicity on Labeling Efficiencies and Nanoparticle Size. Analytical Biochemistry, in press. doi: 10.1016/j.ab.2020.113895
- Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
- Ast, J.; Arvaniti, A.; Fine, N.H.F.; Nasteska, D.; Ashford, F.B.; Stamataki, Z.M Koszegi, Z.; Bacon, A.; Trapp, S.; Jones, B.J.; Hastoy, B.; Tomas, A.; Reissaus, C.; Linnemann, A.K.; D'Este, E.; Calebiro, D.; Johnsson, K.; Podewin, T.; Broichhagen, J.; Hodson, D.J. LUXendins reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. bioRxiv, preprint. doi: 10.1101/557132
- Deol, K.K.; Crowe, S.O.; Du, J.; Bisbee, H.A.; Guenette, R.G.; Strieter, E.R. Proteasome-Bound UCH37/UCHL5 Debranches Ubiquitin Chains to Promote Degradation. Molecular Cell, 2020, 80(5), 796–809.e9. doi: 10.1016/j.molcel.2020.10.017
Cy® is a trademark of GE Healthcare.
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