Cyanine5 maleimide

Maleimides Cyanine5

Cat. #	Quantity	Price	Lead time
13080	1 mg	$125	in stock
23080	5 mg	$260	in stock
43080	25 mg	$510	in stock
53080	50 mg	$895	in stock
63080	100 mg	$1490	in stock

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Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.

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Cyanine5 excitation and emission spectra

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Maleimide Labeling of Proteins and Other Thiolated Biomolecules

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sulfo-Cyanine7.5 is a very hydrophilic and water-soluble dye for the near-infrared region. The absorption and emission spectra of the dye are similar to Indocyanine Green (ICG), but sulfo-Cyanine7.5 has a much higher fluorescence quantum yield. The dye is a great choice for near-infrared in vivo imaging. This is an azide for click chemistry.

AF 555 amine

AF 555 dye with a terminal amino group having strong emission in the yellow region of the spectrum.

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Cyanine3B maleimide

Derivative of a Cyanine3B dye, improved Cyanine3 dye to have higher quantum yield, for maleimide reaction chemistry.

General properties

Appearance:	dark blue powder
Molecular weight:	605.8
CAS number:	1437872-46-2 (without anion)
Molecular formula:	C₃₈H₄₅N₄O₃
IUPAC name:	3H-Indolium, 2-[5-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl-
Solubility:	soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L)
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	646
ε, L⋅mol⁻¹⋅cm⁻¹:	250000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.2
CF₂₆₀:	0.03
CF₂₈₀:	0.04

Product citations

Uckelmann, M.; Levina, V.; Taveneau, C.; Ng, X. H.; Pandey, V.; Martinez, J.; Mendiratta, S.; Houx, J.; Boudes, M.; Venugopal, H.; Trépout, S.; Fulcher, A. J.; Zhang, Q.; Flanigan, S.; Li, M.; Sierecki, E.; Gambin, Y.; Das, P. P.; Bell, O.; de Marco, A.; Davidovich, C. Dynamic PRC1–CBX8 Stabilizes a Porous Structure of Chromatin Condensates. Nat Struct Mol Biol, 2025, 32(3), 520–530. doi: 10.1038/s41594-024-01457-6
Frazier, C.L.; Deb, D.; Weeks, A.M. Engineered reactivity of a bacterial E1-like enzyme enables ATP-driven modification of protein C termini. bioRxiv, 2024, preprint. doi: 10.1101/2024.05.13.593989
Johnson, J.L.; Steele, J.H.; Lin, R.; Stepanov, V.G.; Gavriliuc, M.N.; Wang, Y. Multi-Channel smFRET study reveals a Compact conformation of EF-G on the Ribosome. bioRxiv, 2024, preprint. doi: 10.1101/2024.01.27.577133
Ernst, M.; Orabi, E.A.; Stockbridge, R.B.; Faraldo-Gómez, J.D.; Robertson, J.L. Dimerization mechanism of an inverted-topology ion channel in membranes. Proceedings of the National Academy of Sciences of the United States of America, 2024, 120(47), e2308454120. doi: 10.1073/pnas.2308454120

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Cy® is a trademark of GE Healthcare.

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.