Cyanine5 maleimide

Cat. # Quantity Price Lead time
13080 1 mg $110.00 in stock
23080 5 mg $210.00 in stock
43080 25 mg $410.00 in stock
53080 50 mg $695.00 in stock
63080 100 mg $1190.00 in stock

Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.

Cy5 excitation and emission spectra

Cy5 excitation and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 641.24
CAS number: 1437796-65-0
Molecular formula: C38H45ClN4O3
IUPAC name: 3H-​Indolium, 2-​[5-​[1-​[6-​[[2-​(2,​5-​dihydro-​2,​5-​dioxo-​1H-​pyrrol-​1-​yl)​ethyl]​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Catipovic, M.A.; Rapoport, T.A. Protease protection assays show polypeptide movement into the SecY channel by power strokes of the SecA ATPase. EMBO Reports, in press. doi: 10.15252/embr.202050905
  2. Delvaux, N.A.; Mathew, B.; Rice, K.G. Fluorescent Labeling of Plasmid DNA for Gene Delivery: Implications of Dye Hydrophobicity on Labeling Efficiencies and Nanoparticle Size. Analytical Biochemistry, in press. doi: 10.1016/j.ab.2020.113895
  3. Deol, K.K.; Crowe, S.O.; Du, J.; Bisbee, H.; Guenette, R.G.; Strieter, E.R. Proteasome-Bound UCH37 Debranches Ubiquitin Chains to Promote Degradation. bioRxiv, preprint. doi: 10.1101/2020.02.21.960088
  4. Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
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