Cyanine5 maleimide

Cat. # Quantity Price Lead time
13080 1 mg $110.00 in stock
23080 5 mg $210.00 in stock
43080 25 mg $410.00 in stock
53080 50 mg $695.00 in stock
63080 100 mg $1190.00 in stock

Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.

Cy5 excitation and emission spectra

Cy5 excitation and emission spectra

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Sulfo-Cyanine3 maleimide

Water soluble thiol-reactive derivative of Sulfo-Cyanine3, a Cy3® analog.

Sulfo-Cyanine5.5 amine

Sulfo-Cyanine5.5 is a far red/NIR fluorophore possessing high hydrophilicity and aqueous solubility. The dye displays good brightness in far red region due to outstanding molar extinction coefficient. This is amine derivative which is reactive towards electrophiles. It can also be used for enzymatic transamination labeling.

General properties

Appearance: dark blue powder
Molecular weight: 641.24
CAS number: 1437872-46-2 (without anion), 1437796-65-0 (chloride)
Molecular formula: C38H45ClN4O3
IUPAC name: 3H-​Indolium, 2-​[5-​[1-​[6-​[[2-​(2,​5-​dihydro-​2,​5-​dioxo-​1H-​pyrrol-​1-​yl)​ethyl]​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Ast, J.; Arvaniti, A.; Fine, N.H.F.; Nasteska, D.; Ashford, F.B.; Stamataki, Z.M Koszegi, Z.; Bacon, A.; Trapp, S.; Jones, B.J.; Hastoy, B.; Tomas, A.; Reissaus, C.; Linnemann, A.K.; D'Este, E.; Calebiro, D.; Johnsson, K.; Podewin, T.; Broichhagen, J.; Hodson, D.J. LUXendins reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. bioRxiv, preprint. doi: 10.1101/557132
  2. Singh, R.K.; Fan, J.; Gioacchini, N.; Watanabe, S.; Bilsel, O.; Peterson, C.L. Transient Kinetic Analysis of SWR1C-Catalyzed H2A.Z Deposition Unravels the Impact of Nucleosome Dynamics and the Asymmetry of Histone Exchange. Cell Reports, 2019, 27, 374–386.e4. doi: 10.1016/j.celrep.2019.03.035
  3. Gagni, P.; Romanato, A.; Bergamaschi, G.; Bettotti, P.; Vanna, R.; Piotto, C.; Morasso, C.F.; Chiari, M.; Cretich, M.; Gori, A. A self-assembling peptide hydrogel for ultrarapid 3D bioassays. Nanoscale Advances, 2019, 1(2), 490–497. doi: 10.1039/c8na00158h
  4. Doh, J.K.; White, J.D.; Zane, H.K.; Chang, Y.H.; López, C.S.; Enns, C.A.; Beatty, K.E. VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115, 12961–12966. doi: 10.1073/pnas.1808626115
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