Cyanine5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13080 | 1 mg | $110.00 | in stock | |
| 23080 | 5 mg | $210.00 | in stock | |
| 43080 | 25 mg |
$410.00
|
in stock | |
| 53080 | 50 mg |
$695.00
|
in stock | |
| 63080 | 100 mg |
$1190.00
|
in stock |
Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.
Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.
For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.
Cy5 excitation and emission spectra
Recommended protocol
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Sulfo-Cyanine5 is a water-soluble Cyanine5 dye alkyne for Click chemistry.Cyanine5 carboxylic acid
Cyanine5 carboxylic acid, unactivated dye.
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General properties
| Appearance: | dark blue powder |
| Molecular weight: | 641.24 |
| CAS number: | 1437872-46-2 (without anion), 1437796-65-0 (chloride) |
| Molecular formula: | C38H45ClN4O3 |
| IUPAC name: | 3H-Indolium, 2-[5-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl- |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Gagni, P.; Romanato, A.; Bergamaschi, G.; Bettotti, P.; Vanna, R.; Piotto, C.; Morasso, C.F.; Chiari, M.; Cretich, M.; Gori, A. A self-assembling peptide hydrogel for ultrarapid 3D bioassays. Nanoscale Advances, in press. doi: 10.1039/c8na00158h
- Wu, B.; Zhang, H.; Sun, R.; Peng, S.; Cooperman, B.S.; Goldman, Y.E.; Chen, C. Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots. Nucleic Acids Research, 2018, 46(18), 9736–9748. doi: 10.1093/nar/gky636
- Khor, S.Y.; Vu, M.N.; Pilkington, E.H.; Johnston, A.P.R.; Whittaker, M.R.; Quinn, J.F.; Truong, N.P.; Davis, T.P. Elucidating the Influences of Size, Surface Chemistry, and Dynamic Flow on Cellular Association of Nanoparticles Made by Polymerization-Induced Self-Assembly. Small, 2018, 14(34), e1801702. doi: 10.1002/smll.201801702
- Chambre, L.; Degirmenci, A.; Sanyal, R.; Sanyal, A. Multifunctional Nanogels as Theranostic Platforms: Exploiting Reversible and Non-reversible Linkages for Targeting, Imaging and Drug Delivery. Bioconjugate Chemistry, 2018, 29(6), 1885–1896. doi: 10.1021/acs.bioconjchem.8b00085
Cy® is a trademark of GE Healthcare.
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