AF 343 (Coumarin)-X-NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12620 | 1 mg | $110 | in stock | |
| 22620 | 5 mg | $210 | in stock | |
| 42620 | 25 mg |
$410
|
in stock | |
| 52620 | 50 mg |
$695
|
in stock | |
| 62620 | 100 mg |
$1190
|
in stock |
Blue emitting AF 343 (Coumarin) dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.
This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.
This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.
Absorption and emission spectra of AF 343 (Coumarin)
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A reagent for detecting reactive oxygen species (ROS) in living cells. This carboxylated H2DCFDA analog has two additional negative charges that impede its leakage out of the cell.ROX NHS ester, 5-isomer
Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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BDP® TMR NHS ester
Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.General properties
| Appearance: | yellow solid |
| Molecular weight: | 495.52 |
| CAS number: | 946123-12-2 |
| Molecular formula: | C26H29N3O7 |
| Solubility: | good in DCM, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 437 |
| ε, L⋅mol−1⋅cm−1: | 39000 |
| Emission maximum, nm: | 477 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.29 |
| CF280: | 0.24 |
Product citations
- Zheng, L.; Zhao, H.; Han, Y.; Qian, H.; Vukovic, L.; Mecinović, J.; Král, P.; Huck, W.T.S. Catalytic transport of molecular cargo using diffusive binding along a polymer track. Nature Chemistry, 2019, 11(4), 359–366. doi: 10.1038/s41557-018-0204-7
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/2O
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