Coumarin 343 X NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12620 | 1 mg | $110.00 | in stock | |
| 22620 | 5 mg | $210.00 | in stock | |
| 42620 | 25 mg |
$410.00
|
in stock | |
| 52620 | 50 mg |
$695.00
|
in stock | |
| 62620 | 100 mg |
$1190.00
|
in stock |
Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.
This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.
This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.
Absorption and emission spectra of Coumarin 343
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Amino derivative of BDP FL dye, borondipyrromethene dye for FAM (fluorescein) 488 nm channel.BDP TR X NHS ester
BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.
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EDC, coupling grade
Water soluble carbodiimide for the coupling of carboxylic acids with amines.General properties
| Appearance: | yellow solid |
| Molecular weight: | 495.52 |
| CAS number: | 946123-12-2 |
| Molecular formula: | C26H29N3O7 |
| Solubility: | good in DCM, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 437 |
| ε, L⋅mol−1⋅cm−1: | 39000 |
| Emission maximum, nm: | 477 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.29 |
| CF280: | 0.24 |
Product citations
- Zheng, L.; Zhao, H.; Han, Y.; Qian, H.; Vukovic, L.; Mecinović, J.; Král, P.; Huck, W.T.S. Catalytic transport of molecular cargo using diffusive binding along a polymer track. Nature Chemistry, 2019, 11(4), 359–366. doi: 10.1038/s41557-018-0204-7
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