R6G NHS ester, 5-isomer

Cat. # Quantity Price Lead time
14220 1 mg $110 5 days
24220 5 mg $210 in stock
44220 25 mg $410 in stock
54220 50 mg $695 in stock
64220 100 mg $1190 in stock
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Rhodamine 6G (R6G) is a xanthene dye of rhodamine series that has been used for the labeling of oligonucleotides and DNA for quite a long time. Just like other xanthenes, R6G comes as two isomers, 5- and 6-isomer. Even though their absorption and emission spectra are virtually indistinguishable, the isomers need to be separated to avoid separation of the labeled molecules during their purification, i.e. double HPLC peaks and double spots on electrophoresis.

This is an amine reactive NHS ester derived from a pure 5-isomer of R6G. Rhodamine 6G is very bright (even used as a quantum yield standard, yield 0.95). The dye is soluble in organic solvents, such as DMF, or DMSO. They can be used as co-solvents for the labeling reactions.

Absorption and emission spectra for 5-R6G

Absorption and emission spectra for 5-R6G

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General properties

Appearance: dark colored solid
Molecular weight: 555.58
Molecular formula: C31H29N3O7
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 546
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17
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