R6G phosphoramidite, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| C5260 | 100 mg | – | in stock | |
| D5260 | 250 mg | $190 | in stock | |
| F5260 | 1 g |
$590
|
in stock | |
| G5260 | 5 g |
$1950
|
in stock | |
| H5260 | 10 g | please inquire | in stock |
R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.
R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.
R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.
Usage:
Coupling: 10 minutes.
Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.
Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.
Absorption and emission spectra of 6-R6G
Customers also purchased with this product
BDP 650/665 azide
BDP 650/665 is a red-emitting borondipyrromethene dye. This azide derivative can be conjugated with alkynes and cycloalkynes.ROX azide, 5-isomer
Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 5-isomer.VIC phosphoramidite, 6-isomer
VIC is an asymmetrical xanthene dye with spectral properties similar to HEX and JOE. Useful as a label for qPCR probes.
Add this product to your cart andget free express delivery
General properties
| Appearance: | yellowish powder |
| Molecular weight: | 949.93 |
| CAS number: | 1355330-47-0 |
| Molecular formula: | C46H54F6N5O8P |
| Solubility: | good in DCM, acetonitrile |
| Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 518 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.95 |
| CF260: | 0.18 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
Your cart is empty
$ 
