VIC phosphoramidite, 6-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
BH260 | 50 mg | – | in stock | |
DH260 | 250 mg |
$490
|
in stock | |
FH260 | 1 g |
$990
|
in stock |
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.
Recommendations for using the reagent:
Coupling: 10 minutes.
Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.
Absorption and emission spectra of 6-VIC
![Absorption and emission spectra of 6-VIC](https://static.lumiprobe.us/img/spectra/VIC.png)
Customers also purchased with this product
dsGreen Nucleic Acid Gel Staining Solution, 10000×
dsGreen for staining dsDNA in agarose and polyacrylamide gels.PreciseGreen dsDNA Quantification Reagent, 200×
PreciseGreen is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.Cyanine5 azide
Cyanine5 azide is a reagent for click chemistry labeling with Cyanine5, a popular fluorescent dye.get free express delivery
General properties
Appearance: | white / off white solid |
Molecular weight: | 1023.39 |
CAS number: | 1414265-81-8 |
Molecular formula: | C52H59Cl3N3O10P |
Quality control: | NMR 1H, 31P and HPLC-MS (95+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 525 |
ε, L⋅mol−1⋅cm−1: | 103000 |
Emission maximum, nm: | 546 |
Fluorescence quantum yield: | 0.53 |
CF260: | 0.07 |
CF280: | 0.07 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Deprotection conditions: | standard deprotection conditions |
The count of items is incorrect.
![](/images/delete-row.png)