VIC phosphoramidite, 6-isomer

VIC Phosphoramidites
Cat. # Quantity Price Lead time
BH260 50 mg –   in stock
DH260 250 mg $490 in stock
FH260 1 g $990 in stock
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VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.

This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.

Recommendations for using the reagent:

Coupling: 10 minutes.

Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.

VIC phosphoramidite, 6-isomer, FH260
VIC phosphoramidite, 6-isomer, FH260
VIC phosphoramidite, 6-isomer, FH260
Structure of VIC amidite, 6-isomer
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Absorption and emission spectra of 6-VIC

Absorption and emission spectra of 6-VIC

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General properties

Appearance: white / off white solid
Molecular weight: 1023.39
CAS number: 1414265-81-8
Molecular formula: C52H59Cl3N3O10P
Quality control: NMR 1H, 31P and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 525
ε, L⋅mol−1⋅cm−1: 103000
Emission maximum, nm: 546
Fluorescence quantum yield: 0.53
CF260: 0.07
CF280: 0.07

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Deprotection conditions: standard deprotection conditions
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