VIC phosphoramidite, 6-isomer

Cat. # Quantity Price Lead time
BH260
/ Easily soluble lyophilised form in 8 mL septum
50 mg –   in stock
DH260
/ Easily soluble lyophilised form in 8 mL septum
250 mg $490 in stock
FH260
/ Easily soluble lyophilised form in 30 mL septum
1 g $990 in stock
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VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.

This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.

Recommendations for using the reagent:

Coupling: 10 minutes.

Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.

Absorption and emission spectra of 6-VIC

Absorption and emission spectra of 6-VIC

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FAM phosphoramidite, 6-isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

dsGreen for Real-Time PCR, 100×

Fluorescent dye for detection of amplification in real-time PCR in a ready-to-use 100× stock solution for adding to the master mix.

General properties

Appearance: white / off white solid
Molecular weight: 1023.39
CAS number: 1414265-81-8
Molecular formula: C52H59Cl3N3O10P
Quality control: NMR 1H, 31P and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 525
ε, L⋅mol−1⋅cm−1: 103000
Emission maximum, nm: 546
Fluorescence quantum yield: 0.53
CF260: 0.07
CF280: 0.07

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Deprotection conditions: standard deprotection conditions
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