Cyanine3.5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12030 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 32030 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 42030 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A2030 | 1 mg | $110.00 | in stock | |
| B2030 | 5 mg | $210.00 | in stock | |
| C2030 | 10 mg | $310.00 | in stock | |
| D2030 | 25 mg |
$410.00
|
in stock | |
| E2030 | 50 mg |
$695.00
|
in stock |
Cyanine3.5 labeling reagent, solid material and 10 mM solution in DMSO, for Click Chemistry.
Can replace Cy3.5®, Alexa Fluor 594, DyLight 594.
Cy3.5 absorbance and emission spectra
Recommended protocol
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Coumarin 343 X azide
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.BDP 581/591 azide
BDP 581/591 is a relatively hydrophobic borondipyrromethene fluorophore that can be used as a probe for ROS species, or fluorescence polarization tracer. This is an azide derivative for Click chemistry.General properties
| Appearance: | dark purple powder / solution |
| Molecular weight: | 717.38 |
| Molecular formula: | C44H53ClN6O |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 591 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 604 |
| Fluorescence quantum yield: | 0.35 |
| CF260: | 0.29 |
| CF280: | 0.22 |
Product citations
- Jandl, B.; Sedghiniya, S.; Carstens, A.; Astakhova, K. Peptide–Fluorophore Hydrogel as a Signal Boosting Approach in Rapid Detection of Cancer DNA. ACS Omega, 2019, 4(9), 13889–13895. doi: 10.1021/acsomega.9b01586
- Gaitzsch, J.; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055;. doi: 10.1039/c6py00518g
- Astakhova, I.K.; Wengel, J. Interfacing Click Chemistry with Automated Oligonucleotide Synthesis for the Preparation of Fluorescent DNA Probes Containing Internal Xanthene and Cyanine Dyes. Chemistry - a European Journal, 2013, 19(3), 1112-1122. doi: 10.1002/chem.201202621
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