Cyanine3.5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12030 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 32030 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 42030 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A2030 | 1 mg | $110.00 | in stock | |
| B2030 | 5 mg | $210.00 | in stock | |
| C2030 | 10 mg | $310.00 | in stock | |
| D2030 | 25 mg |
$410.00
|
in stock | |
| E2030 | 50 mg |
$695.00
|
in stock |
Cyanine3.5 labeling reagent, solid material and 10 mM solution in DMSO, for Click Chemistry.
Can replace Cy3.5®, Alexa Fluor 594, DyLight 594.
Cy3.5 absorbance and emission spectra
Recommended protocol
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EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.1-Ethynyl pyrene
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FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for Click Chemistry.General properties
| Appearance: | dark purple powder / solution |
| Molecular weight: | 717.38 |
| Molecular formula: | C44H53ClN6O |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 591 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 604 |
| Fluorescence quantum yield: | 0.35 |
| CF260: | 0.29 |
| CF280: | 0.22 |
Product citations
- Jandl, B.; Sedghiniya, S.; Carstens, A.; Astakhova, K. Peptide–Fluorophore Hydrogel as a Signal Boosting Approach in Rapid Detection of Cancer DNA. ACS Omega, 2019, 4(9), 13889–13895. doi: 10.1021/acsomega.9b01586
- Gaitzsch, J.; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055;. doi: 10.1039/c6py00518g
- Astakhova, I.K.; Wengel, J. Interfacing Click Chemistry with Automated Oligonucleotide Synthesis for the Preparation of Fluorescent DNA Probes Containing Internal Xanthene and Cyanine Dyes. Chemistry - a European Journal, 2013, 19(3), 1112-1122. doi: 10.1002/chem.201202621
Cy® is a trademark of GE Healthcare.
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