Cyanine3.5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12030 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 32030 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 42030 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A2030 | 1 mg | $110.00 | in stock | |
| B2030 | 5 mg | $210.00 | in stock | |
| C2030 | 10 mg | $310.00 | in stock | |
| D2030 | 25 mg |
$410.00
|
in stock | |
| E2030 | 50 mg |
$695.00
|
in stock | |
| F2030 | 100 mg |
$1190.00
|
in stock |
Cyanine3.5 labeling reagent, solid material and 10 mM solution in DMSO, for Click Chemistry.
Can replace Cy3.5®, DyLight 594.
Cy3.5 absorbance and emission spectra
Recommended protocol
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Pyrenebutyric acid NHS ester
Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.Cyanine3 NHS ester
Amine-reactive Cyanine3 dye NHS ester.THPTA ligand
THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.General properties
| Appearance: | dark purple powder / solution |
| Molecular weight: | 675.31 |
| Molecular formula: | C41H47N6ClO |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 591 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 604 |
| Fluorescence quantum yield: | 0.35 |
| CF260: | 0.29 |
| CF280: | 0.22 |
Product citations
- Jandl, B.; Sedghiniya, S.; Carstens, A.; Astakhova, K. Peptide–Fluorophore Hydrogel as a Signal Boosting Approach in Rapid Detection of Cancer DNA. ACS Omega, 2019, 4(9), 13889–13895. doi: 10.1021/acsomega.9b01586
- Gaitzsch, J.; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055;. doi: 10.1039/c6py00518g
- Astakhova, I.K.; Wengel, J. Interfacing Click Chemistry with Automated Oligonucleotide Synthesis for the Preparation of Fluorescent DNA Probes Containing Internal Xanthene and Cyanine Dyes. Chemistry - a European Journal, 2013, 19(3), 1112-1122. doi: 10.1002/chem.201202621
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