Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes
Intended for the CuAAC reaction with proteins, protein labeling buffer protects biomolecules from being damaged by reactive oxygen species. This reaction can be used for proteins with alkyne or azide groups, as well as cells or components of cell lysates metabolically labeled with azide groups.
Azide is conjugated to terminal alkyne, resulting in a five-membered heterocycle (1,2,3-triazole). Both groups (azide and alkyne) are extremely rarely found in natural biomolecules, so the reaction is highly specific and effective to handle various tasks.
The reaction proceeds in the presence of copper (I) compounds and almost does not depend on pH. Optimized for operations with proteins, protein labeling buffer contains a salt of copper (II) (a stable precursor of catalytically-active copper (I) compounds), triethylammonium acetate pH 6.8, water-soluble THPTA ligand, and aminoguanidine. It is recommended to use a freshly prepared solution of ascorbic acid to reduce copper (II). THPTA ligand in protein labeling buffer speeds up the reaction by stabilizing catalytically-active compounds of copper (I). The presence of THPTA ligand also allows protein labeling in water medium (without organic solvents) and, owing to stabilization of copper (I) oxidation degree, minimizes production of reactive oxygen species (RAS) and prevents them from damaging proteins by oxidizing histidine, methionine and cysteine. Aminoguanidine in protein labeling buffer prevents chemically reactive aldehydes (dehydroascorbate hydrolysis products) from binding to side chains of arginine, N-terminal cysteine, and lysine.
For this reaction, you will need alkyne- or azide-modified protein in azide sodium-free buffer, dye azide or alkyne, 1.5х protein labeling buffer, and ascorbic acid. It is recommended to perform steps 6 to 9 under an inert gas (nitrogen or argon).
Protocol
We recommend the following protocol for conjugation of modified proteins with dye derivatives:
- Determine total reaction volume based on the amount of modified protein to be used:
! The volume of alkyne- or azide-modified protein solution should be not more than 1/3 of total
reaction volume.
Total reaction volume, µL Amount of protein 100 4 to 20 nmol 200 20 to 40 nmol 400 40 to 80 nmol 600 80 to 600 nmol - Calculate volumes of the reagents for the labeling reaction using the table below:
* the dye excess may vary depending on the number of azide or alkyne groups on the protein molecule. Calculations in the table are shown for 3x excess of the dye. For 1.5–10x excess of the dye, multiply protein amount (nmol) by 0.15–1. But remember that if you use an azide or alkyne that is not soluble in water, in a big excess it may precipitate from the reaction mixture. Use water-soluble dyes, for example, sulfonated cyanine dyes sulfo-Cyanine.
Reagent Volume, µL Concentration of stock solution Dye azide or alkyne (amount of protein [nmol]) × 0.3* 10 mM in DMSO or water Protein labeling buffer (total reaction volume [µL]) × 0.67 1.5х Activator (ascorbic acid) (total reaction volume [µL]) × 0.02 50 mM in water Water (total reaction volume [µL] – volume of dye solution [µL] – volume of protein labeling buffer [µL] – volume of activator solution [µL]) — - Prepare stock solution of dye azide or alkyne (10 mM in DMSO or water, for water-soluble alkynes and azides) and activator (ascorbic acid, 50 mM in water). Bear in mind that ascorbic acid is readily oxidizable in air. Use only a freshly prepared solution of activator (the solution is stable within 1 day). To prepare stock solution, dissolve 10 mg of ascorbic acid in 1.1 mL of water.
- Add protein labeling buffer to modified protein solution and vortex.
- Add the calculated volume of stock solution of dye azide or alkyne and vortex well again.
- (recommended) Degas the mixture to remove oxygen. To do so, connect a disposable pipette tip to a plastic or silicone tubing connected to the pressure regulator of a gas cylinder with inert gas (argon or nitrogen). Turn on a very weak gas flow and put the tip down in the tube so that it can be 3–10 mm higher than the liquid level avoiding touching the liquid and tube walls. The gas flow should make a swirl in the liquid without spattering it. Keep the tip in this position for 10–20 seconds. If several labeling reactions are run simultaneously, a centrifugal concentrator can be used for degassing. To do so, place the tubes in the concentrator, turn on rotation, turn on vacuum for 30–40 seconds, then turn off vacuum while feeding inert gas to the input of the system.
- Add activator solution (ascorbic acid), then purge the tube with inert gas for a few seconds and close it.
- Vortex the solution.
- Allow the mixture to stand at room temperature for 8–16 h.
- Use dialysis or size-exclusion chromatography to isolate the dye-protein conjugate.
Related products
1-Ethynyl pyrene
Pyrene is a polyaromatic hydrocarbon containing four fused rings. This reagent contains a terminal alkyne group for click chemistry and other reactions, such as Sonogashira coupling.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 515B0 | 50 mg | $50 | in stock | |
| 615B0 | 100 mg | $64 | in stock |
3-Ethynylperylene
Alkyne derivative of ultra-bright and photostable perylene fluorophore.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 555B0 | 50 mg | $100 | in stock | |
| 655B0 | 100 mg | $160 | in stock |
AF 430 alkyne
Alkyne derivative of yellow-green fluorescent dye AF 430 for copper-catalyzed click reaction with azides.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A08B0 | 1 mg | $125 | in stock | |
| B08B0 | 5 mg | $325 | in stock | |
| C08B0 | 10 mg |
$480
|
in stock | |
| D08B0 | 25 mg |
$950
|
in stock | |
| E08B0 | 50 mg |
$1625
|
in stock | |
| F08B0 | 100 mg |
$2090
|
in stock |
AF 488 alkyne
Alkyne derivative of AF 488 dye for copper-catalyzed cycloaddition to azides. Pure 5-isomer.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A18B0 | 1 mg | $125 | in stock | |
| B18B0 | 5 mg | $325 | in stock | |
| D18B0 | 25 mg |
$950
|
in stock | |
| E18B0 | 50 mg |
$1625
|
in stock | |
| F18B0 | 100 mg |
$2090
|
in stock |
AF 488 azide
AF 488 azide is a fluorophore with emission in the green spectrum range. It reacts with alkynes in copper-catalyzed cycloaddition reactions.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| S1830 | 250 ug | – | in stock | |
| 11830 | 1 mg | $125 | in stock | |
| 21830 | 5 mg | $325 | in stock | |
| 41830 | 25 mg |
$950
|
in stock | |
| 51830 | 50 mg |
$1625
|
in stock | |
| 61830 | 100 mg |
$2090
|
in stock |
AF 568 alkyne
AF 568 derivative designed for fluorescence microscopy and flow cytometry. Pure 5-isomer.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A48B0 | 1 mg | $140 | in stock | |
| B48B0 | 5 mg |
$450
|
in stock | |
| C48B0 | 10 mg |
$585
|
in stock | |
| D48B0 | 25 mg |
$1460
|
in stock | |
| E48B0 | 50 mg |
$1890
|
in stock | |
| F48B0 | 100 mg |
$2990
|
in stock |
Alkyne hydrazide
Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 41770 | 25 mg | $110 | in stock | |
| 51770 | 50 mg | $195 | in stock | |
| 61770 | 100 mg | $290 | in stock |
Alkyne maleimide
Maleimide reagent for the attachment of alkyne functional group to proteins, peptides, and other molecules bearing SH (thiol) groups, including cysteine residues.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 41780 | 25 mg | $95 | in stock | |
| 51780 | 50 mg | $175 | in stock | |
| 61780 | 100 mg | $200 | in stock |
Alkyne-PEG2-amine
Diethyleneglycol (PEG2) based bifunctional linker containing amine and alkyne functional groups.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2065-1g | 1 g | $120 | in stock | |
| 2065-5g | 5 g | $350 | in stock | |
| 2065-25g | 25 g | please inquire | in stock |
Alkyne-PEG2-carboxylic acid
Bifunctional diethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2155-1g | 1 g | $380 | in stock |
Alkyne-PEG2-iodide
Bifunctional reactive crosslinker containing terminal alkyne group for click chemistry or Sonogashira coupling, and reactive alkyl iodide group.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2136-1g | 1 g |
$450
|
in stock |
Alkyne-PEG2-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2219-1g | 1 g |
$450
|
in stock | |
| 2219-5g | 5 g |
$980
|
in stock |
Alkyne-PEG3-amine
Alkyne-PEG3-amine is a medium-length heterobifunctional linker for conjugation.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 425-1g | 1 g | $195 | in stock | |
| 425-5g | 5 g |
$580
|
in stock | |
| 425-25g | 25 g | please inquire | in stock |
Alkyne-PEG3-carboxylic acid
Bifunctional triethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1743-1g | 1 g |
$490
|
in stock | |
| 1743-5g | 5 g |
$980
|
in stock | |
| 1743-25g | 25 g | please inquire | in stock |
Alkyne-PEG3-iodide
PEG3 with alkyne and iodide functional groups, a reactive and hydrophilic linker for alkylation and click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2193-1g | 1 g |
$490
|
in stock | |
| 2193-5g | 5 g |
$1150
|
in stock | |
| 2193-25g | 25 g | please inquire | in stock |
Alkyne-PEG3-maleimide
Bifunctional spacer with alkyne and maleimide groups for conjugation of biomolecules.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2344-100mg | 100 mg | $140 | in stock | |
| 2344-1g | 1 g |
$530
|
in stock |
Alkyne-PEG3-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2298-1g | 1 g |
$490
|
in stock | |
| 2298-5g | 5 g |
$980
|
in stock |
Alkyne-PEG3-OH
Alkyne-PEG3-OH is a triethyleneglycol based bifunctional linker with a hydroxyl group and an alkyne fragment.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 418-1g | 1 g | $95 | in stock | |
| 418-5g | 5 g | $280 | in stock | |
| 418-25g | 25 g | please inquire | in stock |
Alkyne-PEG4-amine
A bifunctional linker molecule containing both terminal alkyne moiety for click chemistry, and amine group. Contains a long hydrophilic PEG4 arm.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2025-100mg | 100 mg | $95 | in stock | |
| 2025-1g | 1 g | $390 | in stock |
Alkyne-PEG4-carboxylic acid
Bifunctional tetraethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2187-1g | 1 g | $380 | in stock |
Alkyne-PEG4-iodide
Bifunctional reactive PEG4-based crosslinker containing terminal alkyne group for click chemistry or Sonogashira coupling and reactive alkyl iodide group.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2188-1g | 1 g |
$450
|
in stock |
Alkyne-PEG4-maleimide
Water soluble, heterobifunctional hydrophilic linker with a length of 14 Å, containing maleimide for thiol labeling, and alkyne for click chemistry. An ideal reagent to attach an alkyne moiety to a protein.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2002-100mg | 100 mg | $150 | in stock | |
| 2002-1g | 1 g |
$590
|
in stock |
Alkyne-PEG4-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2409-100mg | 100 mg | $250 | in stock | |
| 2409-1g | 1 g |
$790
|
in stock |
Azide-PEG6-NHS ester
A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3692-250mg | 250 mg | $325 | in stock | |
| 3692-1g | 1 g |
$539
|
in stock |
Azidobutyric acid NHS ester
Activated NHS ester for azido-labeling of peptides and proteins.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 53720 | 50 mg | $110 | in stock | |
| 63720 | 100 mg | $210 | in stock | |
| 73720 | 1 g |
$950
|
in stock | |
| 83720 | 5 g | please inquire | in stock |
BDP 558/568 alkyne
BDP 558/568 is a borondipyrromethene dye for Cyanine3-channel. This product is a terminal alkyne derivative for copper-catalyzed click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A74B0 | 1 mg | $125 | in stock | |
| B74B0 | 5 mg | $260 | in stock | |
| C74B0 | 10 mg | $325 | in stock | |
| D74B0 | 25 mg |
$510
|
in stock | |
| E74B0 | 50 mg |
$895
|
in stock | |
| F74B0 | 100 mg |
$1490
|
in stock |
BDP 581/591 alkyne
BDP 581/591 is a borondipyrromethene fluorophore useful for fluorescence polarization assays, microscopy, and two-photon experiments. This alkyne is a derivative for copper-catalyzed click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A64B0 | 1 mg | $125 | in stock | |
| B64B0 | 5 mg | $260 | in stock | |
| C64B0 | 10 mg | $325 | in stock | |
| D64B0 | 25 mg |
$510
|
in stock | |
| E64B0 | 50 mg |
$895
|
in stock | |
| F64B0 | 100 mg |
$1490
|
in stock |
BDP 630/650 alkyne
Alkyne dye for copper-catalyzed click chemistry, containing borondipyrromethene fluorophore BDP 630/650 for the red channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A54B0 | 1 mg | $125 | in stock | |
| B54B0 | 5 mg | $260 | in stock | |
| C54B0 | 10 mg | $325 | in stock | |
| D54B0 | 25 mg |
$510
|
in stock | |
| E54B0 | 50 mg |
$895
|
in stock | |
| F54B0 | 100 mg |
$1490
|
in stock |
BDP 650/665 alkyne
BDP 650/665 is a bright borondipyrromethene red-emitting dye. The terminal ethynyl group of BDP 650/665 alkyne can be easily conjugated with various azides using a copper-catalyzed click reaction.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A84B0 | 1 mg | $125 | in stock | |
| B84B0 | 5 mg | $260 | in stock | |
| C84B0 | 10 mg | $325 | in stock | |
| D84B0 | 25 mg |
$510
|
in stock | |
| E84B0 | 50 mg |
$895
|
in stock | |
| F84B0 | 100 mg |
$1490
|
in stock |
BDP FL alkyne
Borondipyrromethene dye alkyne for click chemistry. Bright and photostable dye for FAM channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A14B0 | 1 mg | $125 | in stock | |
| B14B0 | 5 mg | $260 | in stock | |
| C14B0 | 10 mg | $325 | in stock | |
| D14B0 | 25 mg |
$510
|
in stock | |
| E14B0 | 50 mg |
$895
|
in stock | |
| F14B0 | 100 mg |
$1490
|
in stock |
BDP R6G alkyne
Terminal alkyne derivative of BDP R6G dye for сlick chemistry. BDP R6G is a borondipyrromethene dye whose absorption and emission spectra are similar to R6G (rhodamine 6G).
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A44B0 | 1 mg | $125 | in stock | |
| B44B0 | 5 mg | $260 | in stock | |
| C44B0 | 10 mg | $325 | in stock | |
| D44B0 | 25 mg |
$510
|
in stock | |
| E44B0 | 50 mg |
$895
|
in stock | |
| F44B0 | 100 mg |
$1490
|
in stock |
BDP TMR alkyne
Borondipyrromethene dye for TAMRA channel, with alkyne group for copper-catalyzed click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A24B0 | 1 mg | $125 | in stock | |
| B24B0 | 5 mg | $260 | in stock | |
| C24B0 | 10 mg | $325 | in stock | |
| D24B0 | 25 mg |
$510
|
in stock | |
| E24B0 | 50 mg |
$895
|
in stock | |
| F24B0 | 100 mg |
$1490
|
in stock |
BDP TR alkyne
BDP TR is a fluorophore for ROX channel. This borondipyrromethene dye is very bright, and resistant to oxidation.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A34B0 | 1 mg | $125 | in stock | |
| B34B0 | 5 mg | $260 | in stock | |
| C34B0 | 10 mg | $325 | in stock | |
| D34B0 | 25 mg |
$510
|
in stock | |
| E34B0 | 50 mg |
$895
|
in stock | |
| F34B0 | 100 mg |
$1490
|
in stock |
Biotin-PEG2-alkyne
Biotin alkyne is a reagent for click chemistry labeling with biotin, an affinity probe to streptavidin.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2546-50mg | 50 mg | $125 | in stock | |
| 2546-100mg | 100 mg | $185 | in stock | |
| 2546-1g | 1 g |
$990
|
in stock |
Biotin-PEG4-alkyne
Biotin alkyne is a reagent for click chemistry labeling with biotin, an affinity probe to streptavidin.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2615-50mg | 50 mg | $125 | in stock | |
| 2615-100mg | 100 mg | $185 | in stock | |
| 2615-1g | 1 g |
$990
|
in stock |
Copper(II)-THPTA catalytic buffer, 1.5x
Ready-to-use catalytic buffer containing сopper(II) and water-soluble THPTA ligand. It is suitable for click chemistry modification of proteins.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| B5150 | 1 mL | $30 | in stock | |
| K5150 | 10 mL | $120 | in stock | |
| S5150 | 50 mL |
$490
|
in stock |
Cyanine3 alkyne
Cyanine3 alkyne is a fluorescent dye alkyne for сlick сhemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A10B0 | 1 mg | $125 | in stock | |
| B10B0 | 5 mg | $260 | in stock | |
| C10B0 | 10 mg | $325 | in stock | |
| D10B0 | 25 mg |
$510
|
in stock | |
| E10B0 | 50 mg |
$895
|
in stock | |
| F10B0 | 100 mg |
$1490
|
in stock |
Cyanine5.5 alkyne
Cyanine5.5 alkyne is a NIR (near infrared) dye azide for copper-catalyzed сlick сhemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A70B0 | 1 mg | $125 | in stock | |
| B70B0 | 5 mg | $260 | in stock | |
| C70B0 | 10 mg | $325 | in stock | |
| D70B0 | 25 mg |
$510
|
in stock | |
| E70B0 | 50 mg |
$895
|
in stock | |
| F70B0 | 100 mg |
$1490
|
in stock |
Cyanine5 alkyne
Cyanine5 alkyne is an alkyne dye for click chemistry labeling of biomolecules.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A30B0 | 1 mg | $125 | in stock | |
| B30B0 | 5 mg | $260 | in stock | |
| C30B0 | 10 mg | $325 | in stock | |
| D30B0 | 25 mg |
$510
|
in stock | |
| E30B0 | 50 mg |
$895
|
in stock | |
| F30B0 | 100 mg |
$1490
|
in stock |
Cyanine7.5 alkyne
Reactive near infrared (NIR) dye for click chemistry containing a terminal alkyne group.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A60B0 | 1 mg | $125 | in stock | |
| B60B0 | 5 mg | $260 | in stock | |
| C60B0 | 10 mg | $325 | in stock | |
| D60B0 | 25 mg |
$510
|
in stock | |
| E60B0 | 50 mg |
$895
|
in stock | |
| F60B0 | 100 mg |
$1490
|
in stock |
Cyanine7 alkyne
Cyanine7 alkyne is a near infrared (NIR) dye alkyne for click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A50B0 | 1 mg | $125 | in stock | |
| B50B0 | 5 mg | $260 | in stock | |
| C50B0 | 10 mg | $325 | in stock | |
| D50B0 | 25 mg |
$510
|
in stock | |
| E50B0 | 50 mg |
$895
|
in stock | |
| F50B0 | 100 mg |
$1490
|
in stock |
FAM alkyne, 5-isomer
FAM alkyne, 5-isomer - an alkyne derivative of fluorescein dye for click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A41B0 | 1 mg | – | in stock | |
| B41B0 | 5 mg | $100 | in stock | |
| C41B0 | 10 mg | $150 | in stock | |
| D41B0 | 25 mg | $295 | in stock | |
| E41B0 | 50 mg |
$450
|
in stock | |
| F41B0 | 100 mg |
$780
|
in stock |
FAM alkyne, 6-isomer
Fluorescein dye alkyne for click chemistry conjugation, pure 6-isomer.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A51B0 | 1 mg | – | in stock | |
| B51B0 | 5 mg | $100 | in stock | |
| C51B0 | 10 mg | $150 | in stock | |
| D51B0 | 25 mg | $295 | in stock | |
| E51B0 | 50 mg |
$450
|
in stock | |
| F51B0 | 100 mg |
$780
|
in stock |
Hexynoic acid NHS ester
Hexynoic acid NHS ester for the labeling of biomolecules with alkyne group for copper-catalyzed click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 41720 | 25 mg | $95 | in stock | |
| 51720 | 50 mg | $180 | in stock | |
| 61720 | 100 mg | $290 | in stock | |
| 91720 | 1 g | please inquire | in stock |
Hexynoic acid STP ester
Alkyne activated ester for the modification of amine groups. STP ester group is an excellent alternative to NHS ester functionality.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 569-25mg | 25 mg | $95 | in stock | |
| 569-100mg | 100 mg | $185 | in stock | |
| 569-500mg | 500 mg |
$850
|
in stock |
JOE alkyne, 6-isomer
JOE dye alkyne derivative for copper-catalyzed click chemistry reaction with azides. JOE dye is widely used for nucleic acid labeling.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A21B0 | 1 mg | $110 | in stock | |
| B21B0 | 5 mg | $210 | in stock | |
| C21B0 | 10 mg | $310 | in stock | |
| D21B0 | 25 mg |
$410
|
in stock | |
| E21B0 | 50 mg |
$695
|
in stock | |
| F21B0 | 100 mg |
$1190
|
in stock |
Pentynoic acid STP ester
An activated ester for the introduction of alkyne into peptides/proteins and click reaction.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 30720 | 10 mg | $110 | in stock | |
| 40720 | 25 mg | $210 | in stock | |
| 50720 | 50 mg |
$410
|
in stock | |
| 60720 | 100 mg |
$595
|
5 days |
R6G alkyne, 6-isomer
R6G (rhodamine 6G) terminal alkyne for copper-catalyzed click chemistry reaction with azides. Pure 6-isomer of the dye.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A52B0 | 1 mg | $110 | in stock | |
| B52B0 | 5 mg | $210 | in stock | |
| C52B0 | 10 mg | $310 | in stock | |
| D52B0 | 25 mg |
$410
|
in stock | |
| E52B0 | 50 mg |
$695
|
in stock | |
| F52B0 | 100 mg |
$1190
|
in stock |
ROX alkyne, 5-isomer
An alkyne derivative of bright red-emitting dye ROX (Rhodamine X, Rhodamine 101) for copper-catalyzed click chemistry bioconjugation. Pure 5-isomer.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A12B0 | 1 mg | $110 | in stock | |
| B12B0 | 5 mg | $165 | in stock | |
| C12B0 | 10 mg | $210 | in stock | |
| D12B0 | 25 mg | $285 | in stock | |
| E12B0 | 50 mg |
$495
|
in stock | |
| F12B0 | 100 mg |
$1190
|
in stock |
ROX alkyne, 6-isomer
An alkyne derivative of bright red-emitting dye ROX (Rhodamine X, Rhodamine 101) for copper-catalyzed click chemistry bioconjugation. Pure 6-isomer.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A22B0 | 1 mg | $110 | in stock | |
| B22B0 | 5 mg | $165 | in stock | |
| C22B0 | 10 mg | $210 | in stock | |
| D22B0 | 25 mg | $285 | in stock | |
| E22B0 | 50 mg |
$495
|
in stock | |
| F22B0 | 100 mg |
$880
|
in stock |
sulfo-Cyanine3.5 alkyne
Alkyne derivative of water-soluble sulfo-Cyanine3.5 dye for copper-catalyzed click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A23B0 | 1 mg | $125 | in stock | |
| B23B0 | 5 mg | $260 | in stock | |
| C23B0 | 10 mg | $325 | in stock | |
| D23B0 | 25 mg |
$510
|
in stock | |
| E23B0 | 50 mg |
$895
|
in stock | |
| F23B0 | 100 mg |
$1490
|
in stock |
sulfo-Cyanine3 alkyne
A water-soluble dye alkyne for copper-catalyzed click chemistry. The fluorophore sulfo-Cyanine3 is a bright and photostable dye for the Cy3® channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A13B0 | 1 mg | $125 | in stock | |
| B13B0 | 5 mg | $325 | in stock | |
| C13B0 | 10 mg |
$470
|
in stock | |
| D13B0 | 25 mg |
$850
|
in stock | |
| E13B0 | 50 mg |
$1490
|
in stock | |
| F13B0 | 100 mg |
$1990
|
in stock |
sulfo-Cyanine5.5 alkyne
Alkyne functionalized, water soluble far red/near infrared emitting fluorophore sulfo-Cyanine5.5.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A73B0 | 1 mg | $110 | in stock | |
| B73B0 | 5 mg | $325 | in stock | |
| C73B0 | 10 mg |
$470
|
in stock | |
| D73B0 | 25 mg |
$850
|
in stock | |
| E73B0 | 50 mg |
$1490
|
in stock | |
| F73B0 | 100 mg |
$2590
|
in stock |
sulfo-Cyanine5 alkyne
sulfo-Cyanine5 is a water-soluble Cyanine5 dye alkyne for click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A33B0 | 1 mg | $125 | in stock | |
| B33B0 | 5 mg | $290 | in stock | |
| C33B0 | 10 mg |
$470
|
in stock | |
| D33B0 | 25 mg |
$850
|
in stock | |
| E33B0 | 50 mg |
$1490
|
in stock | |
| F33B0 | 100 mg |
$1990
|
in stock |
sulfo-Cyanine7.5 alkyne
sulfo-Cyanine7.5 is a near-infrared fluorophore similar to Indocyanine Green (ICG) but possesses higher fluorescence quantum yield. This is an alkyne for copper-catalyzed click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A63B0 | 1 mg | $125 | in stock | |
| B63B0 | 5 mg | $325 | in stock | |
| C63B0 | 10 mg |
$470
|
in stock | |
| D63B0 | 25 mg |
$850
|
in stock | |
| E63B0 | 50 mg |
$1490
|
in stock | |
| F63B0 | 100 mg |
$1990
|
in stock |
sulfo-Cyanine7 alkyne
A sulfonated near-infrared dye alkyne for copper-catalyzed cycloaddition with azides.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A53B0 | 1 mg | $125 | in stock | |
| B53B0 | 5 mg | $325 | in stock | |
| C53B0 | 10 mg |
$470
|
in stock | |
| D53B0 | 25 mg |
$850
|
in stock | |
| E53B0 | 50 mg |
$1490
|
in stock | |
| F53B0 | 100 mg |
$1990
|
in stock |
TAMRA alkyne, 5-isomer
Alkyne derivative of TAMRA dye, pure 5-isomer for сopper-catalyzed сlick chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A71B0 | 1 mg | – | in stock | |
| B71B0 | 5 mg | $260 | in stock | |
| C71B0 | 10 mg | $325 | in stock | |
| D71B0 | 25 mg |
$410
|
in stock | |
| E71B0 | 50 mg |
$695
|
in stock | |
| F71B0 | 100 mg |
$890
|
in stock |
TAMRA alkyne, 6-isomer
Terminal alkyne for copper-catalyzed click chemistry. Tetramethylrhodamine (TAMRA) is a rhodamine dye which is popular in qPCR and other applications. TAMRA is a also a FRET acceptor for FAM.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A81B0 | 1 mg | – | in stock | |
| B81B0 | 5 mg | $260 | in stock | |
| C81B0 | 10 mg | $325 | in stock | |
| D81B0 | 25 mg |
$410
|
in stock | |
| E81B0 | 50 mg |
$695
|
in stock |
The count of items is incorrect.
Your cart is empty
$ 
