sulfo-Cyanine3.5 alkyne

Cat. # Quantity Price Lead time
A23B0 1 mg $125 in stock
B23B0 5 mg $260 in stock
C23B0 10 mg $325 in stock
D23B0 25 mg $510 in stock
E23B0 50 mg $895 in stock
F23B0 100 mg $1490 in stock
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sulfo-Cyanine3.5 is a dye with fluorescence in the orange spectrum range (absorption maximum at 576 nm; emission maximum at 603 nm). Its absorption and emission spectra are between that of Cyanine3 and Cyanine5. Therefore, sulfo-Cyanine3.5 is commonly used as a FRET-donor in a pair with Cyanine5, as a FRET-acceptor in a pair with Cyanine3, and in BRET experiments.

sulfo-Cyanine3.5 is a sulfonated derivative with four sulfo groups, which allows its high hydrophilicity and performing all reactions with this reagent in aqueous solutions without adding organic solvents.

The reagent is an alkyne derivative of sulfo-Cyanine3.5 dye for copper-catalyzed click chemistry. The terminal alkynyl (acetylene) group allows conjugating the fluorophore with the molecules containing an azide group through 1,3-dipolar cycloaddition in the presence of a copper(I) catalyst.

Absorption and emission spectra of sulfo-Cyanine3.5

Absorption and emission spectra of sulfo-Cyanine3.5

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General properties

Appearance: dark colored solid
Mass spec M+ increment: 914.2
Molecular weight: 1028.32
Molecular formula: C41H40N3K3O13S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 576
ε, L⋅mol−1⋅cm−1: 139000
Emission maximum, nm: 603
Fluorescence quantum yield: 0.11
CF260: 0.16
CF280: 0.17
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