AF 488 alkyne

Alkynes AF 488 Click chemistry

Cat. #	Quantity	Price	Lead time
S18B0	250 ug	–	in stock
A18B0	1 mg	$125	in stock
B18B0	5 mg	$325	in stock
D18B0	25 mg	$950	in stock
E18B0	50 mg	$1625	in stock
F18B0	100 mg	$2090	in stock

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Alkyne derivative of AF 488 dye for copper-catalyzed cycloaddition to azides (CuAAC). AF 488 alkyne is used for fluorescent labeling of azidated biomolecules, polymers, and surfaces. AF 488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel (absorption max. is at 495 nm, emission max. is at 519 nm).

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Absorption and emission spectra of AF 488

Recommended protocol

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

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General properties

Appearance:	orange solid
Molecular weight:	647.72
Molecular formula:	C₂₄H₁₅K₂N₃O₁₀S₂
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	495
ε, L⋅mol⁻¹⋅cm⁻¹:	71800
Emission maximum, nm:	519
Fluorescence quantum yield:	0.91
CF₂₆₀:	0.16
CF₂₈₀:	0.10

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.