Alkyne derivative of AF488 dye for copper-catalyzed cycloaddition to azides (CuAAC). AF488 alkyne is useful for fluorescent labeling of azidated biomolecules, polymers, and surfaces. AF488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel (absorption max. is 495 nm, emission max. is 519 nm).
Azidomethyldeoxyuridine (AmdU) is an azide containing nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain promoted Click chemistry.
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General properties
Molecular weight:
773.93
Molecular formula:
C36H47N5O10S2
Quality control:
NMR 1H and HPLC-MS (95+%)
Storage conditions:
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.