AF 532 carboxylic acid

Cat. # Quantity Price Lead time
1C890 1 mg $110 in stock
2C890 5 mg $165 in stock
4C890 25 mg $380 5 days
5C890 50 mg $695 in stock
6C890 100 mg $1190 in stock
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AF 532 is a bright, photostable, and hydrophilic fluorophore emitting in the yellow-green channel, an alternative to HEX, JOE, and SIMA. The dye is useful in super-resolution microscopy, particularly in stochastic optical reconstruction microscopy (STORM), as an activator in nSTORM and a reporter in dSTORM.

AF 532 carboxylic acid is a non-reactive form of AF 532 dye that can be used as a reference standard in experiments involving AF 532 dye conjugates. Besides, the carboxylic group can react with hydrazines, hydroxylamines, and amines using carbodiimides such as EDAC.

Absorption and emission spectra of AF 532

Absorption and emission spectra of AF 532

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General properties

Appearance: reddish-brown powder
Molecular weight: 702.88
Molecular formula: C30H28K2N2O9S2
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 524
ε, L⋅mol−1⋅cm−1: 80500
Emission maximum, nm: 547
Fluorescence quantum yield: 0.6
CF260: 0.17
CF280: 0.11
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