Biotin PEG3 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| C3730 | 10 mg | $40.00 | in stock | |
| D3730 | 25 mg | $90.00 | in stock | |
| E3730 | 50 mg | $140.00 | in stock | |
| F3730 | 100 mg | $180.00 | in stock |
Biotin azide is a derivative of biotin (vitamin H) covalently bound with azide group. This reagent allows labeling of various alkynylated molecules (such as DNA, oligonucleotides, and proteins) with biotin. Biotin binding to avidin or streptavidin can be used in downstream affinity applications for the isolation of biotinylated molecules or their binding with streptavidin conjugates.
This structure of biotin azide features long hydrophilic linker which separates biotin residue from the target molecule for efficient binding with streptavidin. Its linker (PEG) also enhances aquous solubility to facilitate conjugation.
The azide can be conjugated with various molecules using our recommended protocol.
Recommended protocol
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General properties
| Appearance: | colorless solid |
| Molecular weight: | 400.50 |
| CAS number: | 945633-30-7 |
| Molecular formula: | C16H28N6O4S |
| Solubility: | soluble in DMF, DMSO, moderately soluble in water |
| Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. |
| MSDS: | Download |
| Product specifications |
Product citations
- Dettling, S.E.; Ahmadi, M.; Lin, Z.; He, L.; Matthews, M.L. Discovery of Electrophiles and Profiling of Enzyme Cofactors. Current Protocols in Chemical Biology, 2020, 12(4), e86. doi: 10.1002/cpch.86
- Wang, Y.; Dattmore, D.A.; Wang, W:; Pohnert, G.; Wolfram, S.; Zhang, J.; Yang, R.; Decker, E.A.; Lee, K.S.S.; Zhang, G. trans, trans-2,4-decadienal, a lipid peroxidation product, induces inflammatory responses via Hsp90- or 14–3-3ζ-dependent mechanisms. Journal of Nutritional Biochemistry, 2020, 76, 108286. doi: 10.1016/j.jnutbio.2019.108286
- Shen, Y.; Yang, S.; Hu, X.; Zhang, M.; Ma, X.; Wang, Z.; Hou, Y.; Bai, G. Natural product puerarin activates Akt and ameliorates glucose and lipid metabolism dysfunction in hepatic cells. Journal of Functional Foods, 2019, 55, 296–304. doi: 10.1016/j.jff.2019.02.035
- Wang, E.; Hunter, C.P. SID-1 Functions in Multiple Roles To Support Parental RNAi in Caenorhabditis elegans. Genetics, 2017, 207(2), 547–557. doi: 10.1534/genetics.117.300067
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