Biotin PEG3 azide

Cat. # Quantity Price Lead time
C3730 10 mg $40.00 in stock
D3730 25 mg $90.00 5 days
E3730 50 mg $140.00 in stock
F3730 100 mg $180.00 5 days

Biotin azide is a derivative of biotin (vitamin H) covalently bound with azide group. This reagent allows labeling of various alkynylated molecules (such as DNA, oligonucleotides, and proteins) with biotin. Biotin binding to avidin or streptavidin can be used in downstream affinity applications for the isolation of biotinylated molecules or their binding with streptavidin conjugates.

This structure of biotin azide features long hydrophilic linker which separates biotin residue from the target molecule for efficient binding with streptavidin. Its linker (PEG) also enhances aquous solubility to facilitate conjugation.

The azide can be conjugated with various molecules using our recommended protocol.

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1-Ethynyl pyrene

Pyrene is polyaromatic hydrocarbon containing four fused rings. This reagent contains terminal alkyne group for Click Chemistry and other reactions, such as Sonogashira coupling.

General properties

Appearance: colorless solid
Molecular weight: 400.50
CAS number: 945633-30-7
Molecular formula: C16H28N6O4S
Solubility: soluble in DMF, DMSO, moderately soluble in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks.
MSDS: Download
Product specifications

Product citations

  1. Wang, Y.; Dattmore, D.A.; Wang, W:; Pohnert, G.; Wolfram, S.; Zhang, J.; Yang, R.; Decker, E.A.; Lee, K.S.S.; Zhang, G. trans, trans-2,4-decadienal, a lipid peroxidation product, induces inflammatory responses via Hsp90- or 14–3-3ζ-dependent mechanisms. Journal of Nutritional Biochemistry, 2020, 76, 108286. doi: 10.1016/j.jnutbio.2019.108286
  2. Shen, Y.; Yang, S.; Hu, X.; Zhang, M.; Ma, X.; Wang, Z.; Hou, Y.; Bai, G. Natural product puerarin activates Akt and ameliorates glucose and lipid metabolism dysfunction in hepatic cells. Journal of Functional Foods, 2019, 55, 296–304. doi: 10.1016/j.jff.2019.02.035
  3. Wang, E.; Hunter, C.P. SID-1 Functions in Multiple Roles To Support Parental RNAi in Caenorhabditis elegans. Genetics, 2017, 207(2), 547–557. doi: 10.1534/genetics.117.300067
  4. Bruckman, M.A.; Czapar, A.E.; VanMeter, A.; Randolph, L.N.; Steinmetz, N.F. Tobacco mosaic virus-based protein nanoparticles and nanorods for chemotherapy delivery targeting breast cancer. Journal of Controlled Release, 2016, 231, 103–113. doi: 10.1016/j.jconrel.2016.02.045
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