FAM maleimide, 6-isomer

Cat. # Quantity Price Lead time
24180 5 mg –   in stock
44180 25 mg $260.00 in stock
54180 50 mg $370.00 in stock
64180 100 mg $525.00 in stock

FAM (fluorescein) is a bright fluorophore which is compatible with various fluorescence detection instruments. FAM is a universal dye that is useful for microscopy, qPCR and many other methods as well as for FRET-based and fluorescence polarization based binding assays.

FAM maleimide is a thiol reactive dye for the labeling of proteins, peptides, and other thiolated molecules.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

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General properties

Appearance: yellow solid
Mass spec M+ increment: 498.4
Molecular weight: 498.44
Molecular formula: C27H18N2O8
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17

Product citations

  1. Kalinovsky, D. V.; Kholodenko, I. V.; Kibardin, A. V.; Doronin, I. I.; Svirshchevskaya, E. V.; Ryazantsev, D. Y.; Konovalova, M. V.; Rozov, F. N.; Larin, S. S.; Deyev, S. M.; Kholodenko, R. V. Minibody-Based and ScFv-Based Antibody Fragment-Drug Conjugates Selectively Eliminate GD2-Positive Tumor Cells. International Journal of Molecular Sciences, 2023, 24(2), 1239. doi: 10.3390/ijms24021239
  2. Visentin, A.; Demitroff, N.; Salgueiro, M.; Borkosky, S. S.; Uversky, V. N.; Camporeale, G.; de Prat-Gay, G. Assembly of the Tripartite and RNA Condensates of the Respiratory Syncytial Virus Factory Proteins In Vitro: Role of the Transcription Antiterminator M2-1. Viruses, 2023, 15(6), 1329. doi: 10.3390/v15061329
  3. Naeimipour, S.; Boroojeni, F. R.; Lifwergren, P.; Selegård, R.; Aili, D. Multimodal andDynamic Cross-Linking of Modular Thiolated Alginate-Based Bioinks. Materials Today Advances, 2023, 19, 100415. doi: 10.1016/j.mtadv.2023.100415
  4. Kalinovsky, D. V.; Kibardin, A. V.; Kholodenko, I. V.; Svirshchevskaya, E. V.; Doronin, I. I.; Konovalova, M. V.; Grechikhina, M. V.; Rozov, F. N.; Larin, S. S.; Deyev, S. M.; Kholodenko, R. V. Therapeutic Efficacy of Antibody-Drug Conjugates Targeting GD2-Positive Tumors. J Immunother Cancer, 2022, 10(5), e004646. doi: 10.1136/jitc-2022-004646
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