FAM maleimide, 6-isomer

Cat. # Quantity Price Lead time
24180 5 mg –   in stock
44180 25 mg $160.00 in stock
54180 50 mg $270.00 in stock
64180 100 mg $420.00 in stock

FAM (fluorescein) is a bright fluorophore which is compatible with various fluorescence detection instruments. FAM is a universal dye that is useful for microscopy, qPCR and many other methods as well as for FRET-based and fluorescence polarization based binding assays.

FAM maleimide is a thiol reactive dye for the labeling of proteins, peptides, and other thiolated molecules.

Fluorescein (FAM) absorbance and emission spectra

Fluorescein (FAM) absorbance and emission spectra

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Cyanine5 maleimide

Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.
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Sulfo-Cyanine5.5 maleimide

Sulfo-Cyanine5.5 is a water-soluble far red emitting dye. This is a thiol reactive maleimide derivative of sulfo-Cyanine5.5. It is recommended for the labeling of sensitive proteins.

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

General properties

Appearance: yellow solid
Mass spec M+ increment: 498.4
Molecular weight: 498.44
Molecular formula: C27H18N2O8
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Product citations

  1. Woodford, M.R.; Baker-Williams, A.J.; Sager, R.A.; Backe, S.J.; Blanden, A.R.; Hashmi, F.; Kancherla, P.; Gori, A.; Loiselle, D.R.; Castelli, M.; Serapian, S.A.; Colombo, G.; Haystead, T.A.; Jensen, S.M.; Stetler-Stevenson, W.G.; Loh, S.N.; Schmidt, L.S.; Linehan, W.M.; Bah, A.; Bourboulia, D.; Bratslavsky, G.; Mollapour, M. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect. Nature Structural & Molecular Biology, 2021, 28(8), 662–670. doi: 10.1038/s41594-021-00633-2
  2. Nojoumi, S.; Ma, Y.; Schwagerus, S.; Hackenberger, C.P.R.; Budisa, N. In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation. International Journal of Molecular Sciences, 2019, 20(9), 2299. doi: 10.3390/ijms20092299
  3. Sauer, F.; Klemm, T.; Kollampally, R.B.; Tessmer, I.; Nair, R.K.; Popov, N.; Kisker, C. Differential Oligomerization of the Deubiquitinases USP25 and USP28 Regulates Their Activities. Molecular Cell, 2019, 74(3), 421–435.e10. doi: 10.1016/j.molcel.2019.02.029
  4. Yu, C.; Tang, J.; Loredo, A.; Chen, Y.; Jung, S.Y.; Jain, A.; Gordon, A.; Xiao, H. Proximity-Induced Site-Specific Antibody Conjugation. Bioconjugate Chemistry, 2018, 29(11), 3522–3526. doi: 10.1021/acs.bioconjchem.8b00680
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