Sulfo-Cyanine3 alkyne
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A13B0 | 1 mg | $110.00 | in stock | |
| B13B0 | 5 mg | $290.00 | in stock | |
| C13B0 | 10 mg |
$470.00
|
in stock | |
| D13B0 | 25 mg |
$690.00
|
in stock | |
| E13B0 | 50 mg |
$1270.00
|
in stock | |
| F13B0 | 100 mg |
$1990.00
|
in stock |
A water soluble alkyne dye for copper catalyzed Click Chemistry, an analog of Cy3® alkyne.
Sulfo-Cyanine3 is a fluorophore which is compatible with a wide range of fluorescent scanners, imagers, microscopes, and other instrumentation. It is a bright and photostable dye, which is also easily detected in gels by naked eye in low amounts (<1 nmol).
Absorbance and emission spectra of Sulfo-Cyanine3 dye
Recommended protocol
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Cyanine5 maleimide
Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.Coumarin 343 X azide
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
Catalyst for bioconjugate synthesis by Click Chemistry.General properties
| Appearance: | dark red powder |
| Mass spec M+ increment: | 653.2 |
| Molecular weight: | 691.90 |
| CAS number: | 2055138-87-7 (inner salt); 2055138-88-8 (sodium salt) |
| Molecular formula: | C33H38KN3O7S2 |
| Solubility: | soluble in water (0.57 M = 40 g/L), DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Product citations
- Tobola, F.; Sylvander, E.; Gafko, C.; Wiltschi, B. `Clickable lectins': bioorthogonal reactive handles facilitate the directed conjugation of lectins in a modular fashion. Interface Focus, 2019, 9(2), 20180072. doi: 10.1098/rsfs.2018.0072
- Rambarran, T.; Gonzaga, F.; Fatona, A.; Coulson, M.; Saem, S.; Moran-Mirabal, J.; Brook, M.A. Bonding and in-channel microfluidic functionalization using the huisgen cyclization. ournal of Polymer Science Part A: Polymer Chemistry, 2018, 56(6), 589–597. doi: 10.1002/pola.28930
- Konishcheva, E.V.; Zhumaev, U.E.; Meier, W.P. PEO-b-PCL-b-PMOXA Triblock Copolymers: From Synthesis to Microscale Polymersomes with Asymmetric Membrane. Macromolecules, 2017, 50(4), 1512–1520. doi: 10.1021/acs.macromol.6b02743
Cy® is a trademark of GE Healthcare.
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