Sulfo-Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg | $110.00 | in stock | |
| 23320 | 5 mg | $290.00 | in stock | |
| 43320 | 25 mg |
$690.00
|
in stock | |
| 53320 | 50 mg |
$1270.00
|
in stock | |
| 63320 | 100 mg |
$1990.00
|
in stock |
Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.
Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.
Sulfo-Cyanine 5 absorbance and emission spectra
Recommended protocol
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| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| CAS number: | 2230212-27-6 (potassium salt); 937739-58-7 (inner salt) |
| Molecular formula: | C36H40N3KO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Bruniaux, J.; Allard-Vannier, E.; Aubrey, N.; Lakhrif, Z.; Ben Djemaa, S.; Eljack, S.; Marchais, H.; Hervé-Aubert, K.; Chourpa, I.; David, S. Magnetic nanocarriers for the specific delivery of siRNA: contribution of breast cancer cells active targeting for down-regulation efficiency. International Journal of Pharmaceutics, in press. doi: 10.1016/j.ijpharm.2019.118572
- Jang, E.K.; Son, R.G.; Pack, S.P.;. Novel enzymatic single-nucleotide modification of DNA oligomer: prevention of incessant incorporation of nucleotidyl transferase by ribonucleotide-borate complex. Nucleic Acids Research, in press. doi: 10.1093/nar/gkz612
- Gapizov, S.Sh.; Petrovskaya, L.E.; Shingarova, L.N.; Kryukova, E.A.; Boldyreva, E.F.; Lukashev, E.P.; Yakimov, S.A.; Svirshchevskaya, E.V.; Dolgikh, D.A.; Kirpichnikov, M.P. Fusion with an albumin-binding domain improves pharmacokinetics of an αvβ3-integrin binding fibronectin scaffold protein. Biotechnology and Applied Biochemistry, in press. doi: 10.1002/bab.1762
- Zbyszynski, P.; Toraason, I.; Repp, L.; Kwon, G.S. Probing the subcutaneous absorption of a PEGylated FUD peptide nanomedicine via in vivo fluorescence imaging. Nano Convergence, 2019, 6(1), 22. doi: 10.1186/s40580-019-0192-3
Cy® is a trademark of GE Healthcare.
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