Sulfo-Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg | $110.00 | in stock | |
| 23320 | 5 mg | $210.00 | in stock | |
| 43320 | 25 mg |
$410.00
|
in stock | |
| 53320 | 50 mg |
$695.00
|
in stock | |
| 63320 | 100 mg |
$1190.00
|
in stock |
Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.
Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.
Sulfo-Cyanine 5 absorbance and emission spectra
Recommended protocol
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TAMRA fluorescent dye azide for Click chemistry.Sulfo-Cyanine5.5 carboxylic acid
Water soluble far red fluorescent dye sulfo-Cyanine5.5, free non-activated carboxylic acid. Useful for the calibration, and as a non-reactive control.sulfo-Cyanine3.5 NHS ester
Amine reactive activated ester of water soluble, sulfonated sulfo-Cyanine3.5 dye.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| CAS number: | 2230212-27-6 |
| Molecular formula: | C36H40N3KO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Teng, F.-Y.; Wang, T.-T.; Guo, H.-L.; Xin, B.-G.; Sun, B.; Dou, S.-X.; Xi, X.-G.; Hou, X.-M. The HRDC domain oppositely modulates the unwinding activity of E. coli RecQ helicase on duplex DNA and G-quadruplex. The Journal of Biological Chemistry, in press. doi: 10.1074/jbc.RA120.015492
- Ruano-Salguero, J.S.; Lee, K.H. Adsorptive-mediated endocytosis of Sulfo-Cy5-labeled IgG causes aberrant IgG processing by brain endothelial-like cells. Molecular Pharmaceutics, in press. doi: 10.1021/acs.molpharmaceut.0c00712
- Mohammad-Beigi, H.; Hayashi, Y.; Zeuthen, C.M.; Eskandari, H.; Scavenius, C.; Juul-Madsen, K.; Vorup-Jensen, T.; Enghild, J.J.; Sutherland, D.S. Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association. bioRxiv, preprint. doi: 10.1101/2020.02.05.924480
- Janczewska, M.; Szkop, M.; Pikus, G.; Kopyra, K.; Świątkowska, A.; Brygoła, K.; Karczmarczyk, U.; Walczak, J.; Żuk, M.T.; Duszak, J.; Ciach, T. PSMA targeted conjugates based on dextran. Applied Radiation and Isotopes, 2021, 167, 109439. doi: 10.1016/j.apradiso.2020.109439
Cy® is a trademark of GE Healthcare.
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