Sulfo-Cyanine5 NHS ester

Cat. # Quantity Price Lead time
13320 1 mg $110.00 in stock
23320 5 mg $210.00 in stock
43320 25 mg $410.00 in stock
53320 50 mg $695.00 in stock
63320 100 mg $1190.00 in stock

Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.

Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.

This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.

Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.

Sulfo-Cyanine 5 absorbance and emission spectra

Sulfo-Cyanine 5 absorbance and emission spectra

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Sulfo-Cyanine5.5 carboxylic acid

Water soluble far red fluorescent dye sulfo-Cyanine5.5, free non-activated carboxylic acid. Useful for the calibration, and as a non-reactive control.

sulfo-Cyanine3.5 NHS ester

Amine reactive activated ester of water soluble, sulfonated sulfo-Cyanine3.5 dye.

General properties

Appearance: dark blue powder
Molecular weight: 777.95
CAS number: 2230212-27-6
Molecular formula: C36H40N3KO10S2
Solubility: very good in water, good in DMF and DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Teng, F.-Y.; Wang, T.-T.; Guo, H.-L.; Xin, B.-G.; Sun, B.; Dou, S.-X.; Xi, X.-G.; Hou, X.-M. The HRDC domain oppositely modulates the unwinding activity of E. coli RecQ helicase on duplex DNA and G-quadruplex. The Journal of Biological Chemistry, in press. doi: 10.1074/jbc.RA120.015492
  2. Ruano-Salguero, J.S.; Lee, K.H. Adsorptive-mediated endocytosis of Sulfo-Cy5-labeled IgG causes aberrant IgG processing by brain endothelial-like cells. Molecular Pharmaceutics, in press. doi: 10.1021/acs.molpharmaceut.0c00712
  3. Mohammad-Beigi, H.; Hayashi, Y.; Zeuthen, C.M.; Eskandari, H.; Scavenius, C.; Juul-Madsen, K.; Vorup-Jensen, T.; Enghild, J.J.; Sutherland, D.S. Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association. bioRxiv, preprint. doi: 10.1101/2020.02.05.924480
  4. Janczewska, M.; Szkop, M.; Pikus, G.; Kopyra, K.; Świątkowska, A.; Brygoła, K.; Karczmarczyk, U.; Walczak, J.; Żuk, M.T.; Duszak, J.; Ciach, T. PSMA targeted conjugates based on dextran. Applied Radiation and Isotopes, 2021, 167, 109439. doi: 10.1016/j.apradiso.2020.109439
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