Sulfo-Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg | $110.00 | in stock | |
| 23320 | 5 mg | $210.00 | in stock | |
| 43320 | 25 mg |
$410.00
|
in stock | |
| 53320 | 50 mg |
$695.00
|
in stock | |
| 63320 | 100 mg |
$1190.00
|
in stock |
Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.
Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.
Sulfo-Cyanine 5 absorbance and emission spectra
Recommended protocol
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Sulfo-Cyanine5 dUTP
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General properties
| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| CAS number: | 2230212-27-6 (potassium salt); 937739-58-7 (inner salt) |
| Molecular formula: | C36H40N3KO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Walker, J.A.T.; Wang, X.; Peter, K.; Kempe, K.; Corrie, S.R. Dynamic solid-state ultrasound contrast agent for monitoring pH fluctuations in vivo. ACS Sensors, in press. doi: 10.1021/acssensors.0c00245
- Niemeyer, C.M.; Sheshachala, S.; Groesche, M.; Scherr, T.; Hu, Y.; Sun, P.; Bartschat, A.; Mikut, R. Segregation of dispersed silica nanoparticles in microfluidic water in oil droplets: A kinetic study. ChemPhysChem, in press. doi: 10.1002/cphc.201901151
- Li, Q.; Wang, F.; Yang, J.; Liu, D. Direct cytoplasm delivery of gold nanoparticles for real-time apoptosis detection. Nano Research, in press. doi: 10.1007/s12274-020-2707-y
- Mohammad-Beigi, H.; Hayashi, Y.; Zeuthen, C.M.; Eskandari, H.; Scavenius, C.; Juul-Madsen, K.; Vorup-Jensen, T.; Enghild, J.J.; Sutherland, D.S. Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association. bioRxiv, preprint. doi: 10.1101/2020.02.05.924480
Cy® is a trademark of GE Healthcare.
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