sulfo-Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg | $110.00 | in stock | |
| 23320 | 5 mg | $210.00 | in stock | |
| 43320 | 25 mg |
$410.00
|
in stock | |
| 53320 | 50 mg |
$695.00
|
in stock | |
| 63320 | 100 mg |
$1190.00
|
in stock |
Water-soluble Cyanine5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is, therefore, particularly useful for the labeling of proteins that denature in the presence of organic co-solvents, as well as for proteins with low solubility.
sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores, which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, DyLight 649 for all applications.
Sulfo-Cyanine 5 absorbance and emission spectra
Recommended protocol
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Modified deoxy triphosphate containing sulfo-Cyanine5.5 (aka Cy5.5) dye for enzymatic labeling of DNA microarrays, as well as PCR amplicons, and reverse transcripts.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| CAS number: | 2230212-27-6; 146368-14-1 (N-Ethyl) |
| Molecular formula: | C36H40N3KO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Grohme, M.; Frank, O.; Rink, J. Preparing Planarian Cells for High-content Fluorescence Microscopy Using RNA in situ Hybridization and Immunocytochemistry. preprints.org, preprint. doi: 10.20944/preprints202111.0256.v1
- van Hest, J.C.; Song, S.; Mason, A.; Post, R.; de Corato, M.; Mestre, R.; Yewdall, A.; Cao, S.; van der Hofstad, R.; Sanchez, S.; Abdelmohsen, L. Engineering transient dynamics of artificial cells by stochastic distribution of enzymes. ChemRxiv, preprint. doi: 10.33774/chemrxiv-2021-02c1d
- Lee, M.S.; Hyun, H.; Park, I.; Kim, S.; Jang, D.-H.; Kim, S.; Im, J.-K.; Kim, H.; Lee, J.H.; Kwon, T.; Kang, J.H. Quantitative fluorescence in situ hybridization (FISH) of magnetically confined bacteria enables rapid determination of early-stage human bacteremia. medRxiv, preprint. doi: 10.1101/2021.02.19.21251962
- Shea, K.; Koide, H.; Okishima, A.; Hoshino, Y.; Kamon, Y.; Yoshimatsu, K.; Saito, K.; Yamauchi, I.; Ariizumi, S.; Oku, N.; Asai, T.; Zhou, Y.; Xiao, T.-H.; Goda, K. Synthetic hydrogel nanoparticles for sepsis therapy. Research Square, preprint. doi: 10.21203/rs.3.rs-150516/v1
Cy® is a trademark of Cytiva.
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