Sulfo-Cyanine5 NHS ester

Cat. # Quantity Price Lead time
13320 1 mg $110.00 in stock
23320 5 mg $290.00 in stock
43320 25 mg $690.00 in stock
53320 50 mg $1270.00 in stock
63320 100 mg $1990.00 in stock

Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.

Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.

This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.

Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.

Sulfo-Cyanine 5 absorbance and emission spectra

Sulfo-Cyanine 5 absorbance and emission spectra

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Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP is a fluorescent labeled triphosphate for the enzymatic modification of DNA with sulfo-Cyanine5 fluorophore. The reagent contains a long linker arm, its incorporation is highly efficient.
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Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
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General properties

Appearance: dark blue powder
Molecular weight: 777.95
CAS number: 2230212-27-6 (potassium salt); 937739-58-7 (inner salt)
Molecular formula: C36H40N3KO10S2
Solubility: very good in water, good in DMF and DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Barnett, D.; Hall, J.; Haab, B. Automated identification and quantification of signals in multichannel immunofluorescence images: the SignalFinder platform. The American Journal of Pathology, in press. doi: 10.1016/j.ajpath.2019.03.011
  2. Crawford, T.; Fletcher, N.; Veitch, M.; Gonzalez Cruz, J.L.; Pett, N.; Brereton, I.; Wells, J.W.; Mobli, M.; Tesiram, Y. Bacillus anthracis Protective Antigen Shows High Specificity for a UV Induced Mouse Model of Cutaneous Squamous Cell Carcinoma. Frontiers in Medicine, 2019, 6, 22. doi: 10.3389/fmed.2019.00022
  3. Le, D.H.T.; Commandeur, U.; Steinmetz, N.F. Presentation and Delivery of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand via Elongated Plant Viral Nanoparticle Enhances Antitumor Efficacy. ACS Nano, 2019, 13(2), 2501–2510. doi: 10.1021/acsnano.8b09462
  4. Le, D.H.T.; Méndez-López, E.; Wang, C.; Commandeur, U.; Aranda, M.A.; Steinmetz, N.F. Biodistribution of Filamentous Plant Virus Nanoparticles: Pepino Mosaic Virus versus Potato Virus X. Biomacromolecules, 2019, 20(1), 469–477. doi: 10.1021/acs.biomac.8b01365
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