Sulfo-Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg | $110.00 | in stock | |
| 23320 | 5 mg | $290.00 | in stock | |
| 43320 | 25 mg |
$690.00
|
in stock | |
| 53320 | 50 mg |
$1270.00
|
in stock | |
| 63320 | 100 mg |
$1990.00
|
in stock |
Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.
Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.
Sulfo-Cyanine 5 absorbance and emission spectra
Recommended protocol
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Free Cyanine3 carboxylic acid.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| CAS number: | 2230212-27-6 |
| Molecular formula: | C36H40N3KO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Crawford, T.; Fletcher, N.; Veitch, M.; Gonzalez Cruz, J.L.; Pett, N.; Brereton, I.; Wells, J.W.; Mobli, M.; Tesiram, Y. Bacillus anthracis Protective Antigen Shows High Specificity for a UV Induced Mouse Model of Cutaneous Squamous Cell Carcinoma. Frontiers in Medicine, 2019, 6, 22. doi: 10.3389/fmed.2019.00022
- Le, D.H.T.; Commandeur, U.; Steinmetz, N.F. Presentation and Delivery of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand via Elongated Plant Viral Nanoparticle Enhances Antitumor Efficacy. ACS Nano, 2019, 13(2), 2501–2510. doi: 10.1021/acsnano.8b09462
- Le, D.H.T.; Méndez-López, E.; Wang, C.; Commandeur, U.; Aranda, M.A.; Steinmetz, N.F. Biodistribution of Filamentous Plant Virus Nanoparticles: Pepino Mosaic Virus versus Potato Virus X. Biomacromolecules, 2019, 20(1), 469–477. doi: 10.1021/acs.biomac.8b01365
- Pirutin, S.K.; Efremova, M.V.; Yusipovich, A.I.; Turovetskii, V.B.; Maksimov, G.V.; Druzhko, A.B.; Mazhuga, A.G. Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with Prostate-Specific Membrane Antigen in Mouse Macrophages. Bulletin of Experimental Biology and Medicine, 2019, 166(3), 386–389. doi: 10.1007/s10517-019-04356-9
Cy® is a trademark of GE Healthcare.
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