Sulfo-Cyanine5 NHS ester

Cat. # Quantity Price Lead time
13320 1 mg $110.00 in stock
23320 5 mg $210.00 in stock
43320 25 mg $410.00 in stock
53320 50 mg $695.00 in stock
63320 100 mg $1190.00 in stock

Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.

Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.

This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.

Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.

Sulfo-Cyanine 5 absorbance and emission spectra

Sulfo-Cyanine 5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 777.95
CAS number: 2230212-27-6; 146368-14-1 (N-Ethyl)
Molecular formula: C36H40N3KO10S2
Solubility: very good in water, good in DMF and DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Lee, M.S.; Hyun, H.; Park, I.; Kim, S.; Jang, D.-H.; Kim, S.; Im, J.-K.; Kim, H.; Lee, J.H.; Kwon, T.; Kang, J.H. Quantitative fluorescence in situ hybridization (FISH) of magnetically confined bacteria enables rapid determination of early-stage human bacteremia. medRxiv, preprint. doi: 10.1101/2021.02.19.21251962
  2. Chariou, P.L.; Beiss, V.; Ma, Y.; Steinmetz, N.F. In situ vaccine application of inactivated CPMV nanoparticles for cancer immunotherapy. Materials Advances, in press. doi: 10.1039/D0MA00752H
  3. Shea, K.; Koide, H.; Okishima, A.; Hoshino, Y.; Kamon, Y.; Yoshimatsu, K.; Saito, K.; Yamauchi, I.; Ariizumi, S.; Oku, N.; Asai, T.; Zhou, Y.; Xiao, T.-H.; Goda, K. Synthetic hydrogel nanoparticles for sepsis therapy. Research Square, preprint. doi: 10.21203/rs.3.rs-150516/v1
  4. Geng, H.; Zhou, M.; Li, B.; Liu, L.; Yang, X.; Wen, Y.; Yu, H.; Wang, H.; Chen, J.; Chen, L. Metal-drug nanoparticles-mediated osteolytic microenvironment regulation for enhanced radiotherapy of orthotopic osteosarcoma. Chemical Engineering Journal, in press. doi: 10.1016/j.cej.2020.128103
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