sulfo-Cyanine5 NHS ester

Cat. # Quantity Price Lead time
13320 1 mg $91.00 in stock
23320 5 mg $240.00 in stock
43320 25 mg $695.00 in stock
53320 50 mg $895.00 in stock
63320 100 mg $1380.00 in stock

Water-soluble Cyanine5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is, therefore, particularly useful for the labeling of proteins that denature in the presence of organic co-solvents, as well as for proteins with low solubility.

sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores, which is compatible with various equipment such as plate readers, microscopes, and imagers.

This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.

Can be used as a replacement for Cy5®, DyLight 649 for all applications.

Sulfo-Cyanine 5 absorbance and emission spectra

Sulfo-Cyanine 5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 777.95
CAS number: 2230212-27-6; 146368-14-1 (N-Ethyl)
Molecular formula: C36H40N3KO10S2
Solubility: very good in water, good in DMF and DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Martin-Alonso, C.; Tabrizi, S.; Xiong, K.; Blewett, T.; Patel, S.; An, Z.; Sridhar, S.; Bekdemir, A.; Shea, D.; Amini, A. P.; Wang, S.-T.; Kirkpatrick, J.; Rhoades, J.; Golub, T. R.; Love, J. C.; Adalsteinsson, V. A.; Bhatia, S. N. A Nanoparticle Priming Agent Reduces Cellular Uptake of Cell-Free DNA and Enhances the Sensitivity of Liquid Biopsies. bioRxiv, 2023, preprint. doi: 10.1101/2023.01.13.524003
  2. Chen, L.; Xu, N.; Wang, P.; Zhu, H.; Zhang, Z.; Yang, Z.; Zhang, W.; Guo, H.; Lin, J. Nanoalbumin–Prodrug Conjugates Prepared via a Thiolation-and-Conjugation Method Improve Cancer Chemotherapy and Immune Checkpoint Blockade Therapy by Promoting CD8+ T-Cell Infiltration. Bioengineering & Translational Medicine, 2023, 8(1), e10377. doi: 10.1002/btm2.10377
  3. McGregor, L. A.; Zhu, B.; Goetz, A. M.; Sczepanski, J. T. Thymine DNA Glycosylase Is an RNA-Binding Protein with High Selectivity for G-Rich Sequences. Journal of Biological Chemistry, 2023, 299(4), 104590. doi: 10.1016/j.jbc.2023.104590
  4. Mao, X.; Ye, R. One-Pot Synthesis of Enzyme and Antibody/CaHPO4 Nanoflowers for Magnetic Chemiluminescence Immunoassay of Salmonella Enteritidis. Sensors, 2023, 23(5), 2779. doi: 10.3390/s23052779
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