sulfo-Cyanine5 NHS ester
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
13320 | 1 mg | $91 | in stock | |
23320 | 5 mg | $119 | in stock | |
43320 | 25 mg | $345 | in stock | |
53320 | 50 mg | $557 | in stock | |
63320 | 100 mg | $890 | in stock |
Water-soluble Cyanine5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is, therefore, particularly useful for the labeling of proteins that denature in the presence of organic co-solvents, as well as for proteins with low solubility.
sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores, which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, DyLight 649 for all applications.
sulfo-Cyanine5 absorbance and emission spectra
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General properties
Appearance: | dark blue powder |
Molecular weight: | 777.95 |
CAS number: | 2230212-27-6; 146368-14-1 (N-Ethyl) |
Molecular formula: | C36H40N3KO10S2 |
Solubility: | very good in water, good in DMF and DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 271000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.28 |
CF260: | 0.04 |
CF280: | 0.04 |
Product citations
- Geng, T.; Paek, S.Y.; Leung, E.; Chamley, L.W.; Wu, Z. Comparing extracellular vesicles from four different cell origins for intracellular drug delivery to pancreatic cancer cells: Small or large vesicles?. Journal of drug delivery science and technology, 2024, 93, 105416. doi: 10.1016/j.jddst.2024.105416
- Medina, S.; Miller, M. Synthetic Colonic Mucus Enables the Development of Modular Microbiome Organoids. Research Square, 2023. doi: 10.21203/rs.3.rs-3164407/v1
- Liu, Z.; Guo, K.; Yan, L.; Zhang, K.; Wang, Y.; Ding, X.; Zhao, N.; Xu, F.-J. Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms. Nature Communications, 2023, 14, 5132. doi: 10.1038/s41467-023-40830-9
- Fischbach, A.; Johns, A.; Schneider, K.L.; Hao, X.; Tessarz, P.; Nyström, T. Artificial Hsp104-mediated systems for re-localizing protein aggregates. Nature Communications, 2023, 14, 2663. doi: 10.1038/s41467-023-37706-3