Sulfo-Cyanine5 NHS ester

Cat. # Quantity Price Lead time
13320 1 mg $110.00 in stock
23320 5 mg $290.00 in stock
43320 25 mg $690.00 in stock
53320 50 mg $1270.00 in stock
63320 100 mg $1990.00 in stock

Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.

Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.

This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.

Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.

Sulfo-Cyanine 5 absorbance and emission spectra

Sulfo-Cyanine 5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 777.95
CAS number: 2230212-27-6 (potassium salt); 937739-58-7 (inner salt)
Molecular formula: C36H40N3KO10S2
Solubility: very good in water, good in DMF and DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Bruniaux, J.; Allard-Vannier, E.; Aubrey, N.; Lakhrif, Z.; Ben Djemaa, S.; Eljack, S.; Marchais, H.; Hervé-Aubert, K.; Chourpa, I.; David, S. Magnetic nanocarriers for the specific delivery of siRNA: contribution of breast cancer cells active targeting for down-regulation efficiency. International Journal of Pharmaceutics, in press. doi: 10.1016/j.ijpharm.2019.118572
  2. Jang, E.K.; Son, R.G.; Pack, S.P.;. Novel enzymatic single-nucleotide modification of DNA oligomer: prevention of incessant incorporation of nucleotidyl transferase by ribonucleotide-borate complex. Nucleic Acids Research, in press. doi: 10.1093/nar/gkz612
  3. Gapizov, S.Sh.; Petrovskaya, L.E.; Shingarova, L.N.; Kryukova, E.A.; Boldyreva, E.F.; Lukashev, E.P.; Yakimov, S.A.; Svirshchevskaya, E.V.; Dolgikh, D.A.; Kirpichnikov, M.P. Fusion with an albumin-binding domain improves pharmacokinetics of an αvβ3-integrin binding fibronectin scaffold protein. Biotechnology and Applied Biochemistry, in press. doi: 10.1002/bab.1762
  4. Zbyszynski, P.; Toraason, I.; Repp, L.; Kwon, G.S. Probing the subcutaneous absorption of a PEGylated FUD peptide nanomedicine via in vivo fluorescence imaging. Nano Convergence, 2019, 6(1), 22. doi: 10.1186/s40580-019-0192-3
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