Sulfo-Cyanine5 NHS ester

Cat. # Quantity Price Lead time
13320 1 mg $110.00 in stock
23320 5 mg $210.00 in stock
43320 25 mg $410.00 in stock
53320 50 mg $695.00 in stock
63320 100 mg $1190.00 in stock

Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.

Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.

This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.

Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.

Sulfo-Cyanine 5 absorbance and emission spectra

Sulfo-Cyanine 5 absorbance and emission spectra

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Sulfo-Cyanine5 dUTP

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General properties

Appearance: dark blue powder
Molecular weight: 777.95
CAS number: 2230212-27-6 (potassium salt); 937739-58-7 (inner salt)
Molecular formula: C36H40N3KO10S2
Solubility: very good in water, good in DMF and DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Walker, J.A.T.; Wang, X.; Peter, K.; Kempe, K.; Corrie, S.R. Dynamic solid-state ultrasound contrast agent for monitoring pH fluctuations in vivo. ACS Sensors, in press. doi: 10.1021/acssensors.0c00245
  2. Niemeyer, C.M.; Sheshachala, S.; Groesche, M.; Scherr, T.; Hu, Y.; Sun, P.; Bartschat, A.; Mikut, R. Segregation of dispersed silica nanoparticles in microfluidic water in oil droplets: A kinetic study. ChemPhysChem, in press. doi: 10.1002/cphc.201901151
  3. Li, Q.; Wang, F.; Yang, J.; Liu, D. Direct cytoplasm delivery of gold nanoparticles for real-time apoptosis detection. Nano Research, in press. doi: 10.1007/s12274-020-2707-y
  4. Mohammad-Beigi, H.; Hayashi, Y.; Zeuthen, C.M.; Eskandari, H.; Scavenius, C.; Juul-Madsen, K.; Vorup-Jensen, T.; Enghild, J.J.; Sutherland, D.S. Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association. bioRxiv, preprint. doi: 10.1101/2020.02.05.924480
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