sulfo-Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg |
$91
|
in stock | |
| 23320 | 5 mg |
$119
|
in stock | |
| 43320 | 25 mg |
$345
|
in stock | |
| 53320 | 50 mg |
$557
|
in stock | |
| 63320 | 100 mg |
$890
|
in stock |
Water-soluble Cyanine5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is, therefore, particularly useful for the labeling of proteins that denature in the presence of organic co-solvents, as well as for proteins with low solubility.
sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores, which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, DyLight 649 for all applications.
sulfo-Cyanine5 absorbance and emission spectra
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General properties
| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| CAS number: | 2230212-27-6; 146368-14-1 (N-Ethyl) |
| Molecular formula: | C36H40N3KO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Leurs, Y.; Hout, W. V. D.; Gardin, A.; Dongen, J. V.; Hest, J. V.; Grisoni, F.; Brunsveld, L. Automated Navigation of Condensate Phase Behavior with Active Machine Learning. ChemRxiv, 2025, preprint. doi: 0.26434/chemrxiv-2024-frnj3
- Zhao, Z.; Ledezma, D. K.; Affonso de Oliveira, J. F.; Omole, A. O.; Steinmetz, N. F. A Cowpea Mosaic Virus Adjuvant Conjugated to Liposomes Loaded with Tumor Cell Lysates as an Ovarian Cancer Vaccine. Nat Commun, 2025, 16(1), 5047. doi: 10.1038/s41467-025-60239-w
- Soeiro, J. F.; Pereira, R. A.; Oliveira‐Silva, R.; Sousa, F. L.; Gaspar, V. M.; Mano, J. F.; Millán, Á.; Innocenti, C.; Mariani, M.; Lascialfari, A.; Silva, N. J. O. 3D Temperature Mapping During Photothermal Therapy in Cellular Assemblies. Small Structures, 2025, 6(6), 2400658. doi: 10.1002/sstr.202400658
- Zhao, J.; Elgeti, M.; O’Brien, E.S.; Sár, C.P.; EI Daibani, A.; Heng, J.; Sun, X.; White, E.; Che, T.; Hubbell, W.L.; Kobilka, B.K.; Chen, C. Ligand efficacy modulates conformational dynamics of the µ-opioid receptor. Nature, 2024, 629(8011), 474-480. doi: 10.1038/s41586-024-07295-2
Cy® is a trademark of Cytiva.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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