sulfo-Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg | $91.00 | in stock | |
| 23320 | 5 mg | $240.00 | in stock | |
| 43320 | 25 mg |
$695.00
|
in stock | |
| 53320 | 50 mg |
$895.00
|
in stock | |
| 63320 | 100 mg |
$1380.00
|
in stock |
Water-soluble Cyanine5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is, therefore, particularly useful for the labeling of proteins that denature in the presence of organic co-solvents, as well as for proteins with low solubility.
sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores, which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, DyLight 649 for all applications.
Sulfo-Cyanine 5 absorbance and emission spectra
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Bright and photostable, carbonyl reactive borondipyrromethene dye for FAM channel.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| CAS number: | 2230212-27-6; 146368-14-1 (N-Ethyl) |
| Molecular formula: | C36H40N3KO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Martin-Alonso, C.; Tabrizi, S.; Xiong, K.; Blewett, T.; Patel, S.; An, Z.; Sridhar, S.; Bekdemir, A.; Shea, D.; Amini, A. P.; Wang, S.-T.; Kirkpatrick, J.; Rhoades, J.; Golub, T. R.; Love, J. C.; Adalsteinsson, V. A.; Bhatia, S. N. A Nanoparticle Priming Agent Reduces Cellular Uptake of Cell-Free DNA and Enhances the Sensitivity of Liquid Biopsies. bioRxiv, 2023, preprint. doi: 10.1101/2023.01.13.524003
- Chen, L.; Xu, N.; Wang, P.; Zhu, H.; Zhang, Z.; Yang, Z.; Zhang, W.; Guo, H.; Lin, J. Nanoalbumin–Prodrug Conjugates Prepared via a Thiolation-and-Conjugation Method Improve Cancer Chemotherapy and Immune Checkpoint Blockade Therapy by Promoting CD8+ T-Cell Infiltration. Bioengineering & Translational Medicine, 2023, 8(1), e10377. doi: 10.1002/btm2.10377
- McGregor, L. A.; Zhu, B.; Goetz, A. M.; Sczepanski, J. T. Thymine DNA Glycosylase Is an RNA-Binding Protein with High Selectivity for G-Rich Sequences. Journal of Biological Chemistry, 2023, 299(4), 104590. doi: 10.1016/j.jbc.2023.104590
- Mao, X.; Ye, R. One-Pot Synthesis of Enzyme and Antibody/CaHPO4 Nanoflowers for Magnetic Chemiluminescence Immunoassay of Salmonella Enteritidis. Sensors, 2023, 23(5), 2779. doi: 10.3390/s23052779
Cy® is a trademark of Cytiva.
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