FAM isothiocyanate (FITC), 5- and 6-isomers

Cat. # Quantity Price Lead time
3524-100mg 100 mg –   in stock
3524-500mg 500 mg $249 in stock
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FAM (5,6)-isothiocyanate or (5,6)-FITC is an isothiocyanate derivative of fluorescein that reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules. It is often used to label antibodies and other probes for use in various fluorescence-based techniques such as microscopy, flow cytometry, and immunofluorescence assays like immunochemistry, Western blotting, and ELISA.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

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General properties

Appearance: orange powder
Molecular weight: 389.38
CAS number: 27072-45-3
Molecular formula: C21H11NO5S
Solubility: good in DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
Emission maximum, nm: 517

Product citations

  1. Lysakova, E.; Shumeev, A.; Laktyushkin, V.; Chuvpilo, S.; Rybtsov, S. Assay Development for Phagocytosis Activity Evaluation. Biol. Life Sci. Forum, 2024, 31(1), 15. doi: 10.3390/ecm2023-16444
  2. Zhang, Y.; Wang, H.; Zheng, Y.; Wu, Z.; Liu, J.; Cheng, F.; Wang, K. Degradation of Angelica sinensis polysaccharide: Structures and protective activities against ethanol-induced acute liver injury. Carbohydrate polymers, 2024, 328, 121745. doi: 10.1016/j.carbpol.2023.121745
  3. Wu, F.; Ren, Y.; Lv, W.; Liu, X.; Wang, X.; Wang, C.; Cao, Z.; Liu, J.; Wei, J.; Pang, Y. Generating dual structurally and functionally skin-mimicking hydrogels by crosslinking cell-membrane compartments. Nature Communications, 2024, 15(1), 802. doi: 10.1038/s41467-024-45006-7
  4. Luo, T.; Jiang, M.; Cheng, Z.; Lin, Y.; Chen, Y.; Zhang, Z.; Zhou, J.; Zhou, W.; Yu, X.-F.; Li, S.; Geng, S.; Yang, H. Biodegradable FePS3 nanoplatform for efficient treatment of osteosarcoma by combination of gene and NIR-II photothermal therapy. Journal of Nanobiotechnology, 2023, 21, 224. doi: 10.1186/s12951-023-01961-9
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