LumiTracker Mito Rhodamine 123

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2958-1mg 1 mg –   in stock
2958-25mg 25 mg $105 in stock
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Rhodamine 123 (Rh123) is a cationic green-fluorescent xanthene dye used to monitor membrane polarization in mitochondria and bacteria in live cell assays.

The dye distributes according to the negative potential of the membrane. Loss of potential will result in loss of the dye and, therefore, the fluorescence intensity. Like TMRE, Rhodamine 123 can be used to study mitochondrial function changes and cell viability in response to stimuli or pharmaceuticals of interest.

Rhodamine 123 is also used as a tracer dye to determine the rate and direction of membrane transport.

Absorbance and emission spectra of Rh123

 Absorbance and emission spectra of Rh123

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General properties

Appearance: brown crystals
Mass spec M+ increment: 345.30
Molecular weight: 380.83
CAS number: 62669-70-9
Molecular formula: C21H17ClN2O3
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 511
ε, L⋅mol−1⋅cm−1: 86000
Emission maximum, nm: 531
Fluorescence quantum yield: 0.98

Product citations

  1. Li, Z.; Zhang, L.; Zhou, L.; Li, X.; Zhao, Y.; Wang, J. Tunable Catalytic Release of Nitric Oxide via Copper-Loaded Coatings on Titanium Nanotubes for Regulating Biological Performance. Biosurface and Biotribology, 2023, 9(2), 45–57. doi: 10.1049/bsb2.12060
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