LumiTracker Mito JC-1
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
2275-50ug | 50 ug | – | in stock | |
2275-1mg | 1 mg | $110 | in stock | |
2275-5mg | 5 mg | $150 | in stock |
JC-1 is a cationic carbocyanine dye that accumulates in the mitochondria of live cells in a potential-dependent manner.
The dye exists as a green-fluorescent monomer at depolarized membranes and low concentrations. At higher concentrations (aqueous solutions above 0.1 µM) and hyperpolarized membranes, the dye forms J-aggregates that exhibit an emission at the orange channel. The J-aggregates can be excited at 535 nm, and the monomeric form and aggregate together — at 475 nm.
Healthy cells have high mitochondrial membrane potential, and the decrease of mitochondrial membrane potential is a marker of the early stage of apoptosis. All this allows the use of changes in the orange/green fluorescence ratio of JC-1 to determine healthy vs. depolarized mitochondria. The orange/green fluorescence ratio of JC-1 depends only on the mitochondrial membrane potential and not on other factors such as the size, shape, and density of mitochondria.
Absorption and emission spectra of JC-1
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Appearance: | red-purple solid |
Molecular weight: | 652.24 |
CAS number: | 47729-63-5; 3520-43-2 |
Molecular formula: | C25H27Cl4IN4 |
IUPAC name: | 1H-Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-propen-1-yl]-1,3-diethyl-, iodide |
Solubility: | good in DMSO |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |