Biotin-XX-NHS ester

Cat. # Quantity Price Lead time
2897-100mg 100 mg $225 in stock
2897-1g 1 g $670 in stock
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Biotin may be conjugated to many proteins while maintaining the biological activity. The biotinylated probe is usually detected by avidin, streptavidin due to their effective binding which is used in various applications such as affinity chromatography, ELISA and Western Blot, fluorescence-activated cell sorting (FACS), intracellular labeling.

Biotin-XX-NHS ester is a derivative with long spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines. This compound is used to attach biotin to primary amines under alkaline conditions (pH 8-9).

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sulfo-Cyanine3 conjugate of streptavidin, a high-affinity biotin-binding protein, for immunofluorescence, western blots, microplate assays, and other applications use.

General properties

Appearance: light beige solid
Molecular weight: 567.71
CAS number: 89889-52-1
Molecular formula: C26H41N5O7S
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 6-[6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoylamino]hexanoate
Solubility: DMSO, DMF, acetonitrile
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications
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