AF 488 streptavidin

Cat. # Quantity Price Lead time
A18S0 100 ug –   in stock
118S0 1 mg $185 in stock
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Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.

Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.

This streptavidin is a lyophilized conjugate with AF 488, a bright, photostable green fluorophore with spectral characteristics similar to FITC (absorption max. at 495 nm, emission max. at 519 nm).

The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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General properties

Appearance: orange solid
Solubility: good in water
Storage conditions: Transportation: at room temperature for 1 week. Store at -20°С 9 months.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
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