TO-TAP-1, green fluorescent nucleic acid stain

Cat. # Quantity Price Lead time
A3610 50 uL, 5 mM/DMSO $85 in stock
23610 200 uL, 5 mM/DMSO $320 in stock
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TO-TAP-1 (Thiazole Orange Monomer, also known as TO-PRO®-1) is a green fluorescent carbocyanine monomeric dye. TO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make TO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. TO-TAP-1 is non-cytotoxic and may be used for long-term monitoring of cell viability and dead cell detection in culture.

Absorption and emission spectra of TO-TAP-1 (DNA–dye complex)

Absorption and emission spectra of TO-TAP-1 (DNA–dye complex)

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General properties

Appearance: orange solution
Molecular weight: 645.39
CAS number: 157199-59-2
Molecular formula: C24H29I2N3S
IUPAC name: Quinolinium, 4-[(3-methyl-2(3H)-benzothiazolylidene)methyl]-1-[3-(trimethylammonio)propyl]-, diiodide
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 513
Emission maximum, nm: 533
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