AF 647 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| S6830 | 250 ug | – | in stock | |
| A6830 | 1 mg | $140 | in stock | |
| B6830 | 5 mg |
$450
|
in stock | |
| D6830 | 25 mg |
$1460
|
in stock | |
| E6830 | 50 mg |
$1890
|
in stock | |
| F6830 | 100 mg |
$2640
|
in stock |
AF 647 Azide is a fluorescently labeled azide that reacts with alkynyl derivatives of biomolecules (terminal alkynes and cyclooctynes) via click reactions to form stable adducts. AF 647 is a bright, photostable, and hydrophilic fluorophore emitting in the far-red channel (absorption max. is at 650 nm, emission max. is at 671 nm). AF 647 azide is useful for cell labeling, fluorescence microscopy, and flow cytometry.
Absorption and emission spectra of AF 647
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EDC (N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide), coupling grade
Water soluble carbodiimide for the coupling of carboxylic acids with amines.AF 594 azide
Azide derivative of water-soluble red-fluorescent dye AF 594 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.AF 343 (Coumarin)-X-azide
AF 343 (Coumarin) is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by сlick chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility and provides spatial separation between the dye and the biomolecule.General properties
| Appearance: | golden-blue solid |
| Molecular weight: | 1041.37 |
| Molecular formula: | C38H47N6K3O13S4 |
| IUPAC name: | 3-(5-((3-azidopropyl)amino)-5-oxopentyl)-2-((1E,3E)-5-((E)-3,3-dimethyl-5-sulfonato-1-(3-sulfonatopropyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3-methyl-1-(3-sulfonatopropyl)-3H-indol-1-ium-5-sulfonate |
| Solubility: | good in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 655 |
| ε, L⋅mol−1⋅cm−1: | 191800 |
| Emission maximum, nm: | 680 |
| Fluorescence quantum yield: | 0.15 |
| CF260: | 0.09 |
| CF280: | 0.08 |
Product citations
- Murali, V.S.; Rajendran, D.; Isogai, T.; DeBerardinis, R.J.; Danuser, G. RhoA activation promotes glucose uptake to elevate proliferation in MAPK inhibitor resistant melanoma cells. bioRxiv, 2024, preprint. doi: 10.1101/2024.01.09.574940
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/8A
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