AF 647 azide

Cat. # Quantity Price Lead time
S6830 250 ug –   in stock
A6830 1 mg $140 in stock
B6830 5 mg $450 in stock
D6830 25 mg $1460 in stock
E6830 50 mg $1890 in stock
F6830 100 mg $2640 in stock
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AF 647 Azide is a fluorescently labeled azide that reacts with alkynyl derivatives of biomolecules (terminal alkynes and cyclooctynes) via click reactions to form stable adducts. AF 647 is a bright, photostable, and hydrophilic fluorophore emitting in the far-red channel (absorption max. is at 650 nm, emission max. is at 671 nm). AF 647 azide is useful for cell labeling, fluorescence microscopy, and flow cytometry.

Absorption and emission spectra of AF 647

Absorption and emission spectra of AF 647

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General properties

Appearance: golden-blue solid
Molecular weight: 1041.37
Molecular formula: C38H47N6K3O13S4
IUPAC name: 3-(5-((3-azidopropyl)amino)-5-oxopentyl)-2-((1E,3E)-5-((E)-3,3-dimethyl-5-sulfonato-1-(3-sulfonatopropyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3-methyl-1-(3-sulfonatopropyl)-3H-indol-1-ium-5-sulfonate
Solubility: good in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 655
ε, L⋅mol−1⋅cm−1: 191800
Emission maximum, nm: 680
Fluorescence quantum yield: 0.15
CF260: 0.09
CF280: 0.08

Product citations

  1. Murali, V.S.; Rajendran, D.; Isogai, T.; DeBerardinis, R.J.; Danuser, G. RhoA activation promotes glucose uptake to elevate proliferation in MAPK inhibitor resistant melanoma cells. bioRxiv, 2024, preprint. doi: 10.1101/2024.01.09.574940
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