HEX NHS ester, 6-isomer

Cat. # Quantity Price Lead time
2B220 5 mg $175 in stock
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Activated NHS-ester of HEX (Hexachlorofluorescein) dye, a pure 6-isomer. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

HEX is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in the green-yellow area. It can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of their similar spectral characteristics.

HEX NHS ester is widely used in nucleic acid sequencing, PCR/qPCR, and related research.

Absorption and emission spectra of HEX

Absorption and emission spectra of HEX

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General properties

Appearance: light orange powder
Molecular weight: 680.06
CAS number: 2129651-79-0
Molecular formula: C25H9Cl6NO9
Solubility: very soluble in DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 87770
Emission maximum, nm: 549
Fluorescence quantum yield: 0.57
CF260: 0.30
CF280: 0.13
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