HEX NHS ester, 6-isomer
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Activated NHS-ester of HEX (Hexachlorofluorescein) dye, a pure 6-isomer. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.
HEX is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in the green-yellow area. It can serve as a substitute for JOE, BDP™ 530/550, VIC™ because of their similar spectral characteristics.
HEX NHS ester is widely used in nucleic acid sequencing, PCR/qPCR, and related research.
Absorption and emission spectra of HEX
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General properties
Appearance:
light orange powder
Molecular weight:
680.06
CAS number:
2129651-79-0
Molecular formula:
C25 H9 Cl6 NO9
Solubility:
very soluble in DMSO, DMF
Quality control:
NMR 1 H and HPLC-MS (95+%)
Storage conditions:
12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:
Download
Product specifications
Spectral properties
Excitation/absorption maximum, nm:
533
ε, L⋅mol−1 ⋅cm−1 :
87770
Emission maximum, nm:
549
Fluorescence quantum yield:
0.57
CF260 :
0.30
CF280 :
0.13
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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